Tatsunori Matsuzaki
Kansai Medical University
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Featured researches published by Tatsunori Matsuzaki.
Clinical and Applied Thrombosis-Hemostasis | 2000
Shosaku Nomura; Fumiko Okamae; Misao Abe; Mika Hosokawa; Manabu Yamaoka; Tetsuji Ohtani; Shuji Onishi; Tatsunori Matsuzaki; Atsuko Teraoka; Tomoko Ishida; Shirou Fukuhara
The levels of interleukin-6 and platelet-derived microparticles (PMPs) were measured in the blood of 137 pa tients with side effects from platelet concentrate (PC) transfu sion with leukocyte removal filtration, P-selectin-expressing platelet and PMPs in stored PC before and after the filtration, and filtered leukocytes positive for P-selectin glycoprotein li gand-1. The side effects, which were observed in 203 transfu sions for 84 patients with hematologic disease and 53 patients with nonhematologic disease with no significant difference be tween the two groups, included urticaria (75.9%), erythema (18.7%), and fever (17.2%), but no anaphylactic reactions. The levels of interleukin-6 and PMP correlated in both groups, and were significantly higher in the hematologic disease group than in the nonhematologic disease group. The level of PMP, but not interleukin-6, was significantly higher for patients testing posi tive for allergic reaction than for those testing negative. In the stored PC prior to filtration, the level of interleukin-6 was normal. The level of P-selectin-expressing platelets and PMPs was elevated before filtration, but was significantly lower after filtration. Taken together, the results suggest that PMP is in volved in the generation of transfusion reactions, and indicate that both platelets and PMP displaying P-selectin bind to P- selectin glycoprotein ligand-1 of leukocytes retained by the leukocyte filter.
Clinical and Applied Thrombosis-Hemostasis | 2009
Shosaku Nomura; Kazuyoshi Ishii; Norihito Inami; Tatsunori Matsuzaki; Manabu Yamaoka; Fumiaki Urase; Yasuhiro Maeda; Shirou Fukuhara
cells, containing 3.20 × 10 CD34 cells, per kilogram body weight. Hematological engraftment was confirmed 28 days after CBT. Complete chimerism was confirmed by the fluorescence in situ hybridization technique using sex chromosomes. On day 30, the patient’s platelet level was 12 × 10/μL, and the patient became thrombocytopenic and thereafter unresponsive to transfusion of platelets. Although she frequently received platelet transfusions, she died of cerebral bleeding (day 54). Using flow cytometry, the binding of immunoglobulin G (IgG) to the healthy platelets was examined in the patient’s sera. The extent of IgG binding increased in her sera after CBT compared with that before CBT (Figure. 2 A and B). Furthermore, Western blotting revealed that the patient’s sera following CBT had either anti-GPIIb or anti-GPIIIa autoantibodies (Figure. 2C). Immune-mediated thrombocytopenia following SCT can be seen in relation to infection and GVHD, and rarely as an autoimmune phenomenon due to immune dysregulation. Antiplatelet autoantibodies To the Editor: Persistent thrombocytopenia following stem cell transplantation (SCT) has been reported to have a poor survival prognosis. Most cases of SCT-related thrombocytopenia are considered to be associated with chronic graft-versus-host disease (GVHD). However, immune-mediated thrombocytopenia (ITP) following SCT has been suggested to be rare and occurs due to an underlying immune dysregulation mainly associated with GVHD. On the other hand, it has been reported that antiplatelet antibodies are detected in sera of the patients with ITP and that their major target antigen is platelet membrane glycoprotein (GP) IIb-IIIa. Furthermore, CD4 HLA-DR-restricted T cells of ITP proliferate when stimulated by GPIIb-IIIa tryptic peptides or membrane fragments but interestingly not by intact GPIIb-IIIa polypeptide. We herein present the first report of a patient with refractory anemia with excess blast (RAEB) in whom anti-GPIIb-IIIa antibodies were detected after cord blood transplantation (CBT). The patient was a 68-year-old Japanese woman with RAEB that was chemorefractory. There were no HLA-identical sibling donors, and an unrelated umbilical cord blood was prepared. It was confirmed that the patient and the cord blood mismatched at 3 HLA loci (patient: HLA-A 1101/2602, HLA-B 3501/5401, and HLA-DRB1 1501/0410; cord blood: HLA-A 1101/2601, HLA-B 5101/5401, and HLADRB1 1501/0405). The clinical course of CBT is shown in Figure 1. Fludarabine was administered at 35 mg/d from day −5 to day −2, whereas 4 Gy of total body irradiation was given in 2 fractions on day −1. For GVHD prophylaxis, the patient also received cyclosporin at 150 mg/d as a continuous infusion from day 0, 15 mg intravenous methotrexate on day 1 and 10 mg on days 3 and 6, and 45 mg/d prednisolone starting on day 11. In October 2005, she received 2.60 × 10 cryopreserved maternal cord blood stem Clinical and Applied Thrombosis/Hemostasis Volume 15 Number 1 February 2009 123-124
International Journal of Immunogenetics | 2006
Shuichi Nomura; Akira Shouzu; Seitarou Omoto; Tatsunori Matsuzaki; Manabu Yamaoka; Makiko Abe; Masao Hosokawa; Mitsushige Nishikawa; Toshiji Iwasaka; Shirou Fukuhara
We examined the genetic status of human leucocyte antigens (HLA), human platelet alloantigens (HPA) and neutrophil‐specific antigens (NA) in patients with type 2 diabetes mellitus and diabetic arteriosclerosis obliterans (ASO). To our knowledge, the present study is the first report showing the relationship among three genetic factors in type 2 diabetes mellitus and ASO patients. HLA typing was performed by the polymerase chain reaction (PCR)–restriction fragment length polymorphism method. HPA‐typing and NA‐typing were by a PCR‐sequence‐specific primer method. The incidence of HLA‐DRB1*1501 was found to be significant in type 2 diabetes and non‐diabetic, particularly ASO‐positive patients, compared to control subjects. There were no differences in NA1/NA2 between the control and diabetic or non‐diabetic ASO groups. However, the frequency of NA2/NA2 in ASO‐positive diabetes and non‐diabetic ASO patients was significantly higher than controls. The a/b genotype of HPA‐5a/5b was significantly lower in type 2 diabetes and non‐diabetic ASO‐positive patients than in controls. These findings suggest that genetic studies of HLA, NA and HPA could be useful to understand the pathogenesis of type 2 diabetes and ASO.
Autoimmunity | 1999
Shosaku Nomura; Tatsunori Matsuzaki; Manabu Yamaoka; Yoshio Ozaki; Minori Nagahama; Chie Yoshimura; Hideo Kagawa; Shirou Nakayama; Shirou Fukuhara
We performed genetic analysis of human leukocyte antigen (HLA) and human platelet antigen (HPA) in 45 patients with cepharanthin-treated idiopathic thrombocytopenic purpura. HLA-typing was performed by the polymerase chain reaction-restriction fragment length polymorphism method, and HPA-typing by a polymerase chain reaction-sequence-specific primer method. There were 14 responders and 31 nonresponders. Responders included many patients who had already been treated with prednisolone. HLA-DRB1*0901 was significantly more common in responders than in nonresponders. In contrast, HLA-DRB1*0410 and DQB1*0401 were significantly more common in nonresponders. The a/b genotype of HPA-2a/2a (Ko(b)/Ko(b)) was significantly increased in responders. In contrast, HPA-2a/2b (Ko(b)/Ko(a)) and HPA-3a/3b (Bak(a)/Bak(b)) were significantly more common in nonresponders. These findings suggest that genetic studies of HLA and HPA can predict the response of idiopathic thrombocytopenic purpura to cepharanthin.
The American Journal of Chinese Medicine | 1998
Tatsunori Matsuzaki; Shosaku Nomura; Manabu Yamaoka; Yoshio Ozaki; Chie Yoshimura; Gui Lan Xie; Kaoruko Katsura; Hideo Kagawa; Tomoko Ishida; Shirou Fukuhara
We performed human leukocyte antigen (HLA) and human platelet antigen (HPA) in patients with Kami-kihi-to-responsive idiopathic thrombocytopenic purpura. The HLA-A2, A61 and Cw1 were significantly increased in responders compared with nonresponders, as were HLA DRB1 *0901, DRB1 *1502, and DPB1 *0501. In contrast, HLA DPB1 *0201 and DPB1 *0901 were significantly decreased in responders. The a/b genotype of HPA-2 and a/a genotype of HPA-3 were markedly increased in nonresponders, and anti-GPIb antibody was also increased. These results suggest that HLA, HPA, and anti-GP antibody studies may predict the response of idiopathic thrombocytopenic purpura to Kami-kihi-to.
Autoimmunity | 1996
Hideo Kagawa; Shosaku Nomura; Yoshio Ozaki; Kazutaka Uehira; Tatsunori Matsuzaki; Tomoko Ishida; Shiro Fukuhara
Although systemic lupus erythematosus (SLE) is a representative collagen disease, the etiology remains unclear. However, both genetic and environmental factors seem to be involved. Based on serological studies, associations between certain human leukocyte antigens and many autoimmune diseases have long been suggested. Regarding the genetic aspects of SLE, the HLA haplotype is regarded as a potentially important factor, although its role remains controversial. Recently, we examined three family members who had SLE with an identical HLA haplotype.
Journal of the Japan Society of Blood Transfusion | 1996
Shuji Onishi; Shosaku Nomura; Misao Abe; Fumiko Okamae; Manabu Yamaoka; Tetsuji Ohtani; Tatsunori Matsuzaki; Tomoko Ishida; Shirou Fukuhara
We used flow cytometry to investigate the Tk-polyagglutination that was observed in a patient with metastatic lung cancer (59-year-old man). We analyzed the fluorescent patterns of three types erythrocytes obtained from a patient, healthy type-0 subject, and neuraminidase-treatment using four kinds of FITC-conjugated lectins (Griffonia simplicifolia, GS-II; Dolichos biflorus, DBA; Glycine max, SBA; Arachis hypogaea, PNA). Furthermore, the reactivity of normal serum to the patients erythrocytes was analyzed by FITC-conjugated anti-human IgG and IgM antibodies. The patients erythrocytes bound to FITC-GS-II and -PNA, but neuraminidase-treated erythrocytes reacted to FITC-SBA and -PNA. These results were in accordance with those obtained using the aggregation method. However, the reactivity of normal serum to the patients erythrocytes according to flow cytometric analysis was different from that determined by the aggregation method. In particular, anti-human IgM antibody showed the well-reactive tendency. Our results suggest that flow cytometry was useful for the heterogeneous analysis of Tk-polyagglutination in a patient with malignant tumors.
Blood | 1998
Shosaku Nomura; Tatsunori Matsuzaki; Yoshio Ozaki; Manabu Yamaoka; Chie Yoshimura; Kaoruko Katsura; Gui Lan Xie; Hideo Kagawa; Tomoko Ishida; Shirou Fukuhara
Autoimmunity | 1998
Shosaku Nomura; Fumiko Okamae; Tatsunori Matsuzaki; Tomoko Ishida; Shirou Fukuhara
Journal of the Japan Society of Blood Transfusion | 2003
Yuji Kishimoto; Miyoko Arimoto; Mika Hosokawa; Misao Abe; Fumiko Okamae; Manabu Yamaoka; Tetsuji Otani; Tatsunori Matsuzaki; Shuji Onishi; Atsuko Teraoka; Shirou Fukuhara; Hirohide Kawasaki; Yohnosuke Kobayashi; Hirotoshi Shibata