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Dive into the research topics where Tatsuya Munaka is active.

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Featured researches published by Tatsuya Munaka.


international conference on solid state sensors actuators and microsystems | 2003

Integrated micro chamber for living cell analysis with negligible dead volume sample injector

Masaki Kanai; Hirohisa Abe; Tatsuya Munaka; Yoichi Fujiyama; Daisuke Uchida; Asako Yamayoshi; Hiroaki Nakanishi; Akira Murakami; Shuichi Shoji

We develop the micro chamber for real-time monitoring of living cell. It is integrated with an extremely low dead volume sample injector. The chamber was fabricated by the combination of isotropic etching and deep trench etching using CCP-RIE and ICP-RIE. The reproducible sample injection of the order of 1 nL is performed. Biological cell culture of HeLa is also demonstrated in a prototype device.


Japanese Journal of Applied Physics | 2006

Monitoring Cellular Events in Living Mast Cells Stimulated with an Extremely Small Amount of Fluid on a Microchip

Tatsuya Munaka; Hirohisa Abe; Masaki Kanai; Takashi Sakamoto; Hiroaki Nakanishi; Tetsuji Yamaoka; Shuichi Shoji; Akira Murakami

We successfully developed a measurement system for real-time analysis of cellular function using a newly designed microchip. This microchip was equipped with a micro cell incubation chamber (240 nl) and was stimulated by a very small amount of stimuli (as small as 24 nl). Using the microchip system, cultivation of mast cells was successfully carried out. Monitoring of the cellular events after stimulation with an extremely small amount of fluid on a microchip was performed. This system could be applicable for various types of cellular analysis including real-time monitoring of cellular response by stimulation.


Sensors and Actuators A-physical | 2004

Micro chamber for cellar analysis integrated with negligible dead volume sample injector

Masaki Kanai; Hirohsia Abe; Tatsuya Munaka; Yoichi Fujiyama; Daisuke Uchida; Asako Yamayoshi; Hiroaki Nakanishi; Akira Murakami; Shuichi Shoji


Analytical Biochemistry | 2004

Time-resolved luminescence anisotropy-based detection of immunoglobulin G using long-lifetime Ru(II) complex-labeled protein A

Takashi Sakamoto; Atsushi Mahara; Tatsuya Munaka; Koich Yamagata; Reiko Iwase; Tetsuji Yamaoka; Akira Murakami


Biochemical and Biophysical Research Communications | 2015

Isopentenyl pyrophosphate secreted from Zoledronate-stimulated myeloma cells, activates the chemotaxis of γδT cells.

Eishi Ashihara; Tatsuya Munaka; Shinya Kimura; Saori Nakagawa; Yoko Nakagawa; Masaki Kanai; Hideyo Hirai; Hirohisa Abe; Takashi Miida; Susumu Yamato; Shuichi Shoji; Taira Maekawa


Archive | 2013

CELL CULTURE CONTAINER AND CELL CULTURE METHOD USING THE CONTAINER

Tatsuya Munaka; Hirohisa Abe; Masaki Kanai; Masakazu Akechi; Taira Maekawa; Shinya Kimura; Eishi Asihara; Shuichi Shoji; Kentaro Kawai


Analytical Biochemistry | 2006

Real-time monitoring of antibody secretion from hybridomas on a microchip by time-resolved luminescence anisotropy analysis

Tatsuya Munaka; Hirohisa Abe; Masaki Kanai; Takashi Sakamoto; Hiroaki Nakanishi; Tetsuji Yamaoka; Shuichi Shoji; Akira Murakami


Analyst | 2007

Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen

Tatsuya Munaka; Hirohisa Abe; Masaki Kanai; Takashi Sakamoto; Hiroaki Nakanishi; Shuichi Shoji; Shinya Kimura; Taira Maekawa; Akira Murakami


Nucleic acids research. Supplement (2001) | 2003

Detection of acceptor sites for antisense oligonucleotides on native folded RNA by fluorescence-labeled oligonucleotide

Atsushi Mahara; Takashi Sakamoto; Tatsuya Munaka; Reiko Iwase; Tetsuji Yamaoka; Akira Murakami


Sensors and Materials | 2008

Parallel and passive distribution to arrayed microwells using self-regulating pinched flow

Kentaro Kawai; Masaki Kanai; Tatsuya Munaka; Hirohisa Abe; Akira Murakami; Shuichi Shoji

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Akira Murakami

Kyoto Institute of Technology

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Takashi Sakamoto

Japan Advanced Institute of Science and Technology

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Tetsuji Yamaoka

Kyoto Institute of Technology

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