Tatsuya Ohashi
Nitto Boseki
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Publication
Featured researches published by Tatsuya Ohashi.
Clinical Biochemistry | 2008
Takeshi Kawaguchi; Takanari Nakano; Kumiko Sasagawa; Tatsuya Ohashi; Toshihide Miura; Tsugikazu Komoda
Tartrate-resistant acid phosphatase (TRAP) consists of 2 structurally related isoforms, 5a and 5b, and the latter has been characterized as containing a sugar moiety devoid of sialic acid(s). We provide evidence of a greater difference between the sugar moieties of the two isoforms than the presence of sialic acid(s) in TRAP 5a. TRAP 5a and 5b were highly purified from human blood and femur extracts, respectively, and after purifying the TRAPs by successive chromatography on a series of lectin affinity columns, we used the chromatographs obtained to estimate the composition of their sugar chain structure and found that both TRAP 5a and 5b had contained the heterogenous sugar chains. More specifically, TRAP 5a mainly contained a high-mannose-type sugar chain, while TRAP 5b had multi-antennary complex-type sugar chain. These results indicate that the differences between TRAP 5a and 5b consist not only of a difference in modification by sialic acid but differences in other sugar chain structures. These differences are involved in an extraordinary enzyme maturation process since the carbohydrate chains cover the repressive loop where the proteolytic site is located.
Archive | 2006
Toshiki Tamura; Isao Kobayashi; Toshio Kanda; Keiro Uchino; Katsuhiro Katayama; Tatsuya Ohashi; Iwao Kiyokawa; Hisae Arai; Noriyuki Funahashi
Archive | 2003
Fumio Nomura; Kazuyuki Sogawa; Takeshi Tomonaga; Takenori Ochiai; Hideaki Shimada; Tatsuya Ohashi; Katsuhiro Katayama
Archive | 2003
Tatsuya Ohashi; Toshihide Miura; Yoshihiko Igarashi; Kumiko Sasagawa; Katsuhiro Katayama
Archive | 2003
Tatsuya Ohashi; Toshihide Miura; Yoshihiko Igarashi; Fumio Nomura; Takeshi Tomonaga; Katsuhiro Katayama
Archive | 2004
Toshihide Miura; Tatsuya Ohashi; Kumiko Sasagawa; Katsuhiro Katayama
Archive | 2007
Iwao Kiyokawa; Tatsuya Ohashi; Toshihide Miura; Katsuhiro Katayama; Toshiki Tamura; Isao Kobayashi; Hideki Sezutsu
Archive | 2003
Tatsuya Ohashi; Toshihide Miura; Yoshihiko Igarashi; Kumiko Sasagawa; Katsuhiro Katayama
Archive | 2006
Toshiki Tamura; Isao Kobayashi; Toshio Kanda; Keiro Uchino; Katsuhiro Katayama; Tatsuya Ohashi; Iwao Kiyokawa; Hisae Arai; Noriyuki Funahashi
Journal of Electrophoresis | 2008
Yuji Hirano; Akiko Kobayashi; Akira Haruki; Hiroko Haga; Mitsumasa Hara; Toyoji Sato; Tatsuya Ohashi; Kumiko Sasagawa; Toshihide Miura; Yukio Shima; Shinichiro Watanabe