Tatsuyoshi Yamamoto

Thyroglossal duct remnant penetrating the hyoid bone—A case report

Examination of the thyroglossal duct (TGD) in a senile patient with a thyroglossal duct cyst (TGDC), as well as in children, is very valuable in understanding the pathology of TGDC. The precise anatomy of TGDC was studied in a specimen obtained from a 59-year-old man using three-dimensional reconstruction. The authors found the TGD penetrated the hyoid bone. This pathological evidence has not previously appeared in the literature. Penetration of the hyoid bone by the TGD is the result of a forward growth of the hyoid bone after development of a TGD, which had appeared ventral to the hyoid bone. In this gradual forward growth, the hyoid bone had first started to press against, before eventually engulfing, the TGD.

Molecular cloning and characterization of a novel human gene (NESCA) which encodes a putative adapter protein containing SH3

A full-length cDNA encoding a novel protein was isolated and sequenced from a human placental cDNA library. This cDNA consists of 1990 bp and has a predicted open reading frame encoding 433 amino acids. It possesses an Src homology 3 (SH3) motif, a leucine zipper motif and no catalytic domain, suggesting that it seems to be an adapter protein. PCR-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels placed the gene to human chromosome 1q21-22.

Treatment of a giant inguinal hernia using transabdominal pre-peritoneal repair

We present the case of a male Japanese patient with a giant inguinal hernia that extended to his knees while standing. A transabdominal pre-peritoneal (TAPP) repair was performed under general anesthesia. Complete reduction of the contents of the hernia was achieved within 2 h 50 min. A blood loss of approximately 700 ml was noted. The patient was discharged from the hospital on post-operative Day 12, with no recurrence of the hernia 6 months post-surgery. Factors contributing to the successful outcomes included preparation of several reduction methods before surgery, use of a large size mesh and implementation of pre-operative measures to prevent abdominal compartment syndrome. Further studies are required to evaluate the feasibility of laparoscopic repair in the management of giant inguinal hernia.

Intercept-PCR, an improvement for elevating performance to find a new member of a certain gene family.

We have established a method by which the performance of reverse transcriptase coupled polymerase chain reaction (RT-PCR) for seeking a new gene is improved. The actual procedure is quite easy: it is only to add several specific oligonucleotides into the reaction mixture of the usual RT-PCR. To verify the effectiveness of this method is also easy: it is only to detect the PCR products in the preliminary experiment. The finding in the present study provides valuable information for gene cloning tactics.