Tayeb Idoui

Protective effects of probiotic Lactobacillus plantarum BJ0021 on liver and kidney oxidative stress and apoptosis induced by endosulfan in pregnant rats.

Abstract Endosulfan (EDS) is one of the most widely organochlorine insecticide used in many parts of the world, although it is currently banned or severely restricted in use in some countries. EDS causes a variety of negative effects in non-target species including humans. Therefore, the aim of the present study was to investigate the possible protective effects of Lactobacillus plantarum BJ0021 on toxicity, oxidative stress, and apoptosis induced by EDS intoxication on liver and kidneys of pregnant rats. This pesticide induced a significant increase in total cholesterol, alanine-amino transferase (ALAT), aspartate-amino transferase (ASAT), alkaline phosphatase (ALP), gamma-glutamyl transpeptidase (GGT), urea and creatinine in serum, while urinary urea and creatinine were lower than those of the control group. In the liver and kidney, lipid peroxidation increased significantly, the antioxidant levels, such as superoxide dismutase (SOD) and catalase (CAT) were markedly depressed and TdT-mediated dUTP-biotin Nick End Labeling (TUNEL) revealed more apoptotic cells. In contrast, co-administration of L. plantarum BJ0021 to EDS-treated animals ameliorated most of these biochemical parameters, but the activity of antioxidant enzymes (SOD, CAT) did not modify and the number of apoptotic nuclei remained significantly raised in kidney compared to control. In conclusion, the administration of L. plantarum BJ0021 decreased apoptosis and might play a protective role in reducing toxicity of EDS in pregnant rats.

Lactobacillus strains: Screening of probiotics properties in vitro and in vivo

During the study period 2011 2013, 14 multicase families have been identified from unique screening of 1084 houses from Andhra Pradesh and Odisha states. In all multicase families, all first identified individuals were noted as MB. A total of 34 cases were successfully screened from multicases families, SSS samples were obtained, DNA was isolated and subjected to PCR-VNTR analysis. Families1, 7, 11, 13 have copy number 4 and families -4, 14 have copy number 5 for locus (GGT) 5. Most of the families have copy number 2 for locus 21 3. The copy number 9 of (GTA) 9 have linked with households of family1, 13, 14 and copy number 13 has seen in family 4. Copy number 11of (AT) 17 locus has coupled with households of family1, 4 and copy number 12, 13 have associated with families 13 and 14. Copy number 8 of (AC) 9 locus has associate with households of family1; 9 copy number in 4 and 14 families; 10 copy number in family 13. For 6 7 locus, households of family1, 3, 6 have 7 copy number; households of families -2, 5, 12, 13 and 14 have copy number 6; 5 copy number has seen in family4. Households of family1, 6, 8, 13 and 14 have 5 copy number; households of family -4 has copy number 4; 6 copy number has seen in family5 and 12 for locus 27 5. 23 3 locus has copy number 2 in multicase families. 12 copy number of (TTC) 21 locus has connected with households of families1, 13 and 14; families2 and 4 have copy number 10. Multicase families those have same copy number of VNTR loci have been infected with leprosy patient in the households. The multicase families whose have different copy number have infected with leprosy bacilli from the environment.T feeding trial was carried out to evaluate the effects of different dietary commercial probiotic (pediococcus accidilactici) and folic acid on the immune system in Ship sturgeon (Acipenser nudiventiris). 210 fish averaging 12.84±1.53 (mean SD) were randomly distributed into each of twenty one rectangular plastic tanks of 50 L capacity. Each tank was then randomly assigned to one of three replicates of seven diets containing control diet, 1(4 mg folic acid kg-1 diet), 2(300 g probiotics Tone-1 diet), 3(4 mg folic acid kg-1 diet+200 g Tone-1 diet), 4 (2 mg folic acid kg-1 diet+300 g Tone-1 diet), 5(2 mg folic acid kg-1 diet+200 g Tone-1 diet), 6 (4 mg folic acid kg-1 diet+300 g Tone-1 diet). At the end of eighteen weeks of feeding trial, both haematological indices and leucocyte counts were significantly (P < 0.05) affected by dietary pediococcus accidilactici and Folic acid and their combinations on diet. In the group with 4 mg FA+3 g pediococcus accidilactici, lysozyme activity and total immunoglobulin (Ig) level significantly increased compared to the those groups fed other diets (P < 0.05). Moreover, the fish fed with diets supplemented with 2 mg FA+ 3 g pediococcus accidilactici per kg-1 diet had considerably higher immunoglobulin (IgM) level (P < 0.05). Under the experimental conditions, dietary pediococcus accidilactici and FA had a synergistic effect on enhancing growth performance and immunity of Acipenser nudiventris.J Fonolla1, J A Maldonado-Lobon1, M Gil-Campos2, J Maldonado3, K Flores2, M R Benavides4, R Jaldo5, I Jimenez del Barco6, V Bolivar5, E Prados5, I Penalver5, M Molina5, J M Fernandez5, J L Garcia-Salmeron5, F J Garrido5, A D Valero3, F Lara-Villoslada7 and M Olivares3 1Biosearch Life, Spain 2Hospital Reina Sofía, Spain 3Hospital Universitario Virgen de las Nieves, Spain 4Clínica Pediátrica Roquetas, Spain 5Servicio Andaluz de Salud, Spain 6Policlínica Cristo de la Salud, Spain 7Lactalis Puleva, SpainD the study period 2011-2013, 14 multi-case families have been identified from unique screening of 1084 houses from Andhra Pradesh and Odisha states. In all multi-case families, all first identified individuals were noted as MB. A total of 34 cases were successfully screened from multi-cases families, SSS samples were obtained and DNA was isolated and subjected to PCR-VNTR analysis. Families-1, 7, 11, 13 have copy number 4 and families-4, 14 have copy number 5 for locus (GGT) 5. Most of the families have copy number 2 for locus 21-3. The copy number 9 of (GTA) 9 has linked with households of family-1, 13, 14 and copy number 13 has seen in family 4. Copy number 11of (AT) 17 loci has coupled with households of family-1, 4 and copy number 12, 13 have associated with families 13 and 14. Copy number 8 of (AC) 9 locus has associate with households of family-1; 9 copy number in 4 and 14 families; 10 copy number in family13. For 6-7 locus, households of family-1, 3, 6 have 7 copy number; households of families -2, 5, 12, 13 and 14 have copy number 6; 5 copy number has seen in family-4. Households of family-1, 6, 8, 13 and 14 have 5 copy number; households of family-4 has copy number 4; 6 copy number has seen in family-5 and 12 for locus 27-5. 23-3 locus has copy number 2 in multi-case families. 12 copy number of (TTC) 21 loci have connected with households of families-1, 13 and 14; families-2 and 4 have copy number 10. Multi-case families those have same copy number of VNTR loci have been infected with leprosy patient in the households. The multi-case families whose have different copy number have infected with leprosy bacilli from the environment.

Technological and Probiotic Traits of the Lactobacilli Isolated From Vaginal Tract of the Healthy Women for Probiotic Use

Background For biotechnological application, selected lactic acid bacteria strains belonging to the genera Lactobacillus (Lb) are proposed as an alternative to the antibiotics for the prevention and treatment of urogenital tract infections. Objectives Isolating and selecting vaginal lactobacilli strains for probiotic use based on their technological and probiotic aptitudes. Materials and Methods The vaginal isolates were examined for their essential characteristics as the potential probiotic such as low pH tolerance, bile-salt and simulated human intestinal fluid (SIF) resistance, adhesion to the vaginal epithelial cells (VECs), aggregation and coaggregation, surface hydrophobicity, antimicrobial activity, acid production, antibiotic resistance, and resistance to spermicides. The best strain was identified by PCR. Results From 70 lactobacilli isolates and according to the 16 rDNA sequences, isolates B6 and B10 showed the closest homology (99%) to the Lb. gasseri and Lb. plantarum respectively. They produced hydrogen peroxide (H2O2), tolerant to acid, bile, simulated human intestinal fluid, present a strong adhesion, highest percentages of aggregation, and antibacterial activity. These strains are resistant to the spermicide and actively acidify the growth medium. Conclusions Strains Lb. plantarum B10 and Lb. gasseri B6 have a strong potential probiotic confirming their value as a tool for prevention against urinary and vaginal infections.

Novel Isolates of Lactobacilli from Crop of Algerian Poultry as Potential Probiotic for Food Industry

This study was aimed at selecting novel strains of Lactobacillus from crop of Algerian poultry. One hundred forty (140) lactobacilli strains were isolated and examined for their potentiality probiotic properties. From these isolated strains, nine appear to possess a probiotic value and highlighted a noticeable heterogeneity. The isolate L. plantarum G 1 showed the best inhibitory activity against several indicator strains. Furthermore, the results showed that culture and neutralized supernatants exhibited varying degrees of inhibitory activity against strains of enterobacteria from poultry origin. The tested strains were acid resistant and were also bile tolerant. Antibiotic resistance, co-aggregation activity and hydrophobicity percentage were strain-dependent. Moreover, six strains were able to adhere to epithelial cells. Finally, six Lactobacillus strains, such as strain L. plantarum G 1, L. plantarum PC 2 , L. viridesencs G 3 , L. helveticus PC 6 , L. delbrueckii ssp delbrueckii G 7 and L. fermentum PC 8 , showed essential probiotic properties. The identity of the best strain L. plantarum G1 was confirmed by 16S rRNA gene sequence using PCR.