Tengfei Zhao
Northeast Forestry University
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Publication
Featured researches published by Tengfei Zhao.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2012
Qingyong Li; Qiaochu Zhu; Xiaoqiu Deng; Wuna He; Tengfei Zhao; Baoyou Zhang
In this study, the binding interactions of the water-soluble camptothecin derivatives hydroxycamptothecin (10-HCPT), topotecan (TPT), and camptothecin quaternary salt (CPT8), to bovine serum albumin (BSA) were determined using fluorescence spectra and UV-vis spectra. The results revealed that the fluorescence of BSA was strongly quenched by the binding of camptothecin derivatives to BSA. The quenching mechanism of the camptothecin derivatives was found to be static according to the Stern-Volmer equation. The binding constant and binding sites were confirmed by fluorescence quenching spectra. The thermodynamic parameters Gibbs free energy change (ΔG<0), enthalpy change (ΔH>0), and entropy change (ΔS>0) implied that the interaction process was spontaneous and endothermic, and the interaction forces between camptothecin compounds and BSA were found to be hydrophobic. According to Föster non-radioactive energy transfer, the binding distances between 10-HCPT, TPT, and CPT8, and BSA were determined to be 1.73nm, 1.63nm, and 1.61nm, respectively. The synchronous fluorescence spectra confirmed that the camptothecin compounds cannot cause conformational changes in BSA. A rapid and sensitive method for determining the binding interaction between water-soluble camptothecin derivatives and BSA was established based on these principles of fluorescence quenching.
Molecules | 2011
Qingyong Li; Wei Qiu; Qiaochu Zhu; Yuangang Zu; Xiaoqiu Deng; Tengfei Zhao; Chunfei Jiang; Li Zhang
Camptothecin-20(s)-O-[N-(3’α,12’α-dihydroxy-24’-carbonyl-5’β-cholan)]-lysine (B2) is a novel camptothecin analogue. Our previous study had shown that it displayed higher cytoxicity activity towards hepatocellular carcinoma SMMC-7721 cells than camptothecin (CPT) in vitro. In this paper, the underlying mechanism of anti-proliferation of B2 towards SMMC-7721 cells was further examined. Cell growth inhibition of B2 was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; morphological changes were observed under Laser Scanning Confocal Microscope (LSCM); cell cycle distribution, apoptotic population, changes in mitochondrial membrane potential, intracellular calcium concentration and reactive oxygen species (ROS) production were determined by flow cytometry (FCM). Activities of caspase-3 and caspase-9 were measured, and the expression level of Bcl-2 and Bax proteins were analyzed by Western blot. The results suggested that B2 inhibited SMMC-7721 cell growth by causing cell cycle arrest at the S and G2/M phases, and induced apoptosis involving a mitochondrial pathway. B2 appears to cause a high induction of apoptosis on SMMC-7721 cells in vitro, which suggests it might be a potential drug for cancer therapy.
Fitoterapia | 2013
Qingyong Li; Qiaochu Zhu; Xueke Zhang; Changlong Hua; Xiaoqiu Deng; Tengfei Zhao; Baihe Sun
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to identify and quantify the camptothecin quaternary derivative CPT8 for application in pharmacokinetics and tissue distribution studies. Rat plasma and tissue samples were extracted with methanol by using camptothecin as the internal standard (IS). Chromatographic separation of CPT8 and the IS was achieved using a Hypersil GOLD C18 column, with a flow rate of 1.0 mL/min followed by quantification with tandem mass spectrometry, operating with electrospray ionization in the positive ion mode and by applying multiple reaction monitoring. The MS/MS ion transitions were monitored at m/z 484.3-361.2 for CPT8 and m/z 349.0-305.2 for the IS (CPT). A calibration curve was constructed using CPT8 concentrations ranging from 2.5 ng/mL to 2500 ng/mL (r>0.993). The efficiency of CPT8 extraction from plasma and tissue samples ranged from 91.23% to 105.4%. Intra- and inter-day precision (relative standard deviation) values were 0.21% and 7.25%, respectively. No matrix effects were observed. The freeze-thaw stability, post-extraction stability, and stability following short- and long-term storage at low temperatures ranged from 84.12% to 108.2%. The preclinical data obtained using this method is expected to facilitate future clinical investigations of CPT8.
Journal of Drug Targeting | 2014
Qingyong Li; Tianyu Liu; Yunchao Li; Shuyue Luo; Qiaochu Zhu; Li Zhang; Tengfei Zhao
Abstract Camptothecin-20(s)-O-glycine ester-[N-(3′α, 12′α-dihydroxy-24′-carbonyl-5′β-cholan)] (A2), 10-(3′α,12′α-dihydroxy-5′β-cholan-24′-carboxyl)-(20 s)-camptothecin (C2), and 10-O-(3-O-(3′α, 12′α-dihydroxy-24′-carbonyl-5′β-cholan)-propyl)-(20S)-camptothecin (D2) are novel camptothecin-deoxycholic acid analogues. MTT assays were performed to assess the anticancer activity of these compounds against hepatocellular carcinoma SMMC-7721, breast carcinoma MCF-7, and colorectal carcinoma HCT-116 cells. A2 had a high killing ability on SMMC-7721 cells selectively, but C2 and D2 did not exhibit selectivity with regard to SMMC-7721 killing. Uptake assays were performed in an effort to elucidate the transport mechanisms of A2 into SMMC-7721 cells. A2 increased the mRNA expression of OATP1B3 (an organic anion-transporting polypeptide) and uptake of A2 was inhibited by rifampin (inhibitor of OATP1B3), which indicated that the transporter-mediated transport of A2 was mediated by OATP1B3. In addition, according to the western blot and apoptosis assays, we found that A2 killed SMMC-7721 cells by inducing cell apoptosis mainly via an AIF (apoptosis-inducing factor) pathway and a caspase-dependent mitochondria apoptosis pathway.
Letters in Drug Design & Discovery | 2012
Qingyong Li; Wuna He; Li Zhang; Yuangang Zu; Qiaochu Zhu; Xiaoqiu Deng; Tengfei Zhao; Wenqing Gao; Baoyou Zhang
Archive | 2011
Qingyong Li; Yuangang Zu; Yang Gao; Baoyou Zhang; Wuna He; Tengfei Zhao; Xiaoqiu Deng
Archive | 2011
Qingyong Li; Yang Gao; Yuangang Zu; Baoyou Zhang; Wuna He; Tengfei Zhao; Xiaoqiu Deng; Chichu Zhu
Archive | 2011
Qingyong Li; Yuangang Zu; Wuna He; Wei Qiu; Baoyou Zhang; Tengfei Zhao; Wenqing Gao; Xiaoqiu Deng
Archive | 2011
Qingyong Li; Yuangang Zu; Wuna He; Wei Qiu; Baoyou Zhang; Tengfei Zhao; Wenqing Gao; Xiaoqiu Deng
Archive | 2011
Qingyong Li; Wuna He; Wei Qiu; Baoyou Zhang; Tengfei Zhao; Wenqing Gao; Xiaoqiu Deng