Teresa Bachanek

Interrelationships between morphometric, densitometric, and mechanical properties of mandible in 6-month-old male pigs

Abstract The aim of the study was to evaluate interrelationships between final body weight, and morphometric, densitometric, and mechanical properties of the mandible in 6-month-old Polish Large White pigs exposed to dexamethasone and nanocalcium. The study was performed on 27 males, castrated on the 28th d after weaning, and reared until the age of 6 months, after which the animals were slaughtered, and the mandible was obtained. The pigs were divided into four experimental groups: animals given per os nanopartical calcium, animals injected with dexamethasone, animals given both nanopartical calcium per os and dexamethasone injections, and animals injected with placebo. After the slaughter, morphological properties of the mandible such as bone weight and length were determined. Using computed tomography technique, volumetric bone mineral density (vBMD) of the cortical bone (Cd), mean volumetric bone mineral density (MvBMD), and total bone volume (Bvol) of whole mandible were measured. Areal bone mineral density (BMD) and bone mineral content were evaluated with the use of dual-energy X-ray absorptiometric method. Using three-point bending test, mechanical parameters such as maximum elastic strength (Wy) and ultimate strength (Wf) of mandible were estimated. Pearson’s correlation coefficient (r) was determined between all the investigated variables. The obtained results showed a significantly positive correlation between body weight and mandible weight, mandible length, Bvol, Cd, BMD, BMC, Wy, and Wf. However, statistically insignificant correlations of MvBMD and body weight, mandible weight, mandible length, and Bvol were observed. Furthermore, Bvol and Cd were not found to be significantly correlated. In conclusion, this study showed numerous positive correlations between final body weight and densitometric, morphometric, and mechanical properties of the mandible. This bone of pigs may be used as an attractive model for further investigation on metabolic response of the skeleton to physiological, nutritional, toxicological, and pharmacological factors influencing bone tissue metabolism.

Enzymes in therapy of biofilm-related oral diseases.

Biofilm‐related infections of the oral cavity, including dental caries and periodontitis, represent the most prevalent health problems. For years, the treatment thereof was largely based on antibacterial chemical agents. Recently, however, there has been growing interest in the application of more preventive and minimally invasive biotechnological methods. This review focuses on the potential applications of enzymes in the treatment and prevention of oral diseases. Dental plaque is a microbial community that develops on the tooth surface, embedded in a matrix of extracellular polymeric substances of bacterial and host origin. Both cariogenic microorganisms and the key components of oral biofilm matrix may be the targets of the enzymes. Oxidative salivary enzymes inhibit or limit the growth of oral pathogens, thereby supporting the natural host defense system; polysaccharide hydrolases (mutanases and dextranases) degrade important carbohydrate components of the biofilm matrix, whereas proteases disrupt bacterial adhesion to oral surfaces or affect cell–cell interactions. The efficiency of the enzymes in in vitro and in vivo studies, advantages and limitations, as well as future perspectives for improving the enzymatic strategy are discussed.

The role of probiotics in prevention of oral diseases.

The dynamic development of knowledge in the field of probiotics was commenced at the beginning of the 20th century. Since then, many ways of their possible usage in medicine have been established. In accordance with the WHO, probiotics are live microorganisms, which if applied in adequate amounts may benefit the host. Among probiotics, fungi and bacteria are distinguished, and mechanisms of action of these organisms in the oral cavity and gut are parallel. Application in dentistry, in prophylaxis and treatment of oral diseases is still not well known. Most commonly, Lactobacillus reuteri, Lactobacillus rhamnosus GG and Bifidobacterium are applied. The aim of the study was to collect and systematize the latest information about probiotics and their role in pathomechanisms of dental caries, gingivitis and periodontitis, candidiasis, and malodour. Based on the analyzed literature, it can be concluded that mechanisms of cariogenic pathogen inhibition using probiotics are still not well understood. The new research trend is based on application of probiotics which can naturally displace cariogenic bacteria in the oral cavity and influence oral health in adults and children. The results of studies also confirmed the beneficial role of probiotics in reduction of the bacterial population in periodontitis and halitosis. Long-term observation and a properly designed study protocol will allow us to answer many questions concerning substitution of one strain of bacteria by another.

Characteristics of oral probiotics – a review

Abstract Probiotics are a group of microorganisms able to have a positive influence on a host organism when applied in adequate amounts. They are grouped either as: bacteria (mainly Lactobacillus spp and Bifidobacterium) or fungi (Saccharomyces boulardii). Recent studies have revealed many opportunities for their use in several fields of medicine, such as in: reducing the level of cholesterol in the body, cancer therapy, human immune system regulation, skin regeneration, pancreas necrosis, cirrhosis of liver treatment, regulation of post- antibiotic bowel function, constipation and digestive disorders in infants. Probiotics efficacy has also been demonstrated in oral cavity malfunctions. With the use of modern scientific methods, probiotics have the potential to become an important part of the daily diet and a natural drug supplementation in severe diseases.

Assessment of Pain Intensity in Patients with Dentin Hypersensitivity After Application of Prophylaxis Paste Based on Calcium Sodium Phosphosilicate Formula

Background One of many functions of the pulp-dentin complex is sensory function. Acute, situated, receding pain after the cessation of the stimulus action is called dentin pain. Dentin hypersensitivity has been described as one of the most painful and least successfully treated chronic ailments of teeth. The aim of this research was the clinical evaluation of the effectiveness of professional polishing paste containing calcium sodium phosphosilicate formula (NovaMin) in eliminating dentin hypersensitivity after a single application. Material/Methods The study comprised 92 teeth with dentin hypersensitivity diagnosed on the basis of history and clinical examination. The pain reaction of exposed dentine was induced by tactile and dehydrating stimuli, asking patients to assess the severity of pain on the VAS scale. Clinical trial and survey were carried out twice: before and 1 week after the application of the polishing paste. Results After the application of the examined paste, the percentage of teeth reacting with a severe pain to the touch of the probe decreased from 16.3% to 4.3%, and with a moderate pain from 42.4% to 12%. Examination after applying dehydrating stimulus a week after carrying out the application showed a decrease in the proportion of teeth with strong pain from 28.3% to 0% and moderate pain from 38% to 15.2%. The lack of pain increased from 12% to about 50%. Conclusions The use of prophylactic professional paste with NovaMin formula in in-office procedure provides the reduction of dentin hypersensitivity noticeable by 1 week after application.

Qualitative analysis of the margins of restorations made with different filling resins

Purpose: In this study the comparative results obtained from examinations of two different restorative composites with different cavities filling method are presented. Micro‐CT was used for the evaluation of the marginal integrity and quality of composite fillings in connection with microstructural investigations made by both optical and scanning electron microscopy.

Caries pattern in three-year old preschool children

Background. Analysis of the results of epidemiological studies of Polish children showed that caries in children can occur very early, and their incidence and intensity increase with age. According to an epidemiological study, 53.8% children at the age of 3 reported an average of 2.4 tooth decay. There is insufficient information about the intensity of decay, the pattern of caries in primary dentition and the treatment undertaken in the group of 3-years-old children with tooth decay.

The condition of oral mucosa in the elderly (over 65 years) of Lublin

Abstract Mucous membrane defense mechanisms are impaired with age, both immunologically and physically. This decreases oral mucosa regenerative capability and results in greater susceptibility to injuries and microbial and fungal colonization. Pathological changes of the mucous membrane should be diagnosed and treated early, as some may develop into cancerous changes. Therefore, regular dental check-ups are essential, especially in old age. The aim of the study was to assess the condition of the oral mucosa in seniors residing in either Lublin Nursing Homes (LNH) or in home care. The study was conducted among 240 people over the age of 65: 117 LNH residents and 123 seniors living independently. The assessment of oral mucosa was conducted via clinical examination so as to see evidence of pathological change. On the base of the performed examination, pathological changes of the oral mucosa were observed in 43.59% of all LNH residents and in 34.96% of all seniors living in home care (independently). In both groups, atrophic glossitis, candidiasis (in susp.) and stomatitis prothetica were most frequently noted. Hence, it can be said that the condition of the oral mucosa of the examined seniors from Lublin is unsatisfactory.

The condition of mineralized tooth tissue of the inhabitants of Szackie Lake District (Ukraine) with regard to the content of silver and chosen microelements in drinking water and the soil

INTRODUCTION AND OBJECTIVE The aim of the study is to evaluate the mineralization tooth tissues in the inhabitants of Melniki village Szackie, Lake District, Ukraine, with regard to silver content and chosen macro-elements in the soil, tap water, and the water in Pisoczno Lake located in the vicinity of village. MATERIAL AND METHODS The macro-elements, silver ions contained in the lake, tap water and soil were examined. 125 people aged 12-73, indigenous inhabitants of Melniki village, were qualified for dental examinations. The research took into account the distance between the place of residence and Pisoczno Lake as a source of silver ions, dividing the examined patients into 2 groups: A) living at a distance less than 2,500 m from the lake, and B) living at a distance more than 2.500 m from the lake. RESULTS In area A, significantly higher contents of phosphorus and potassium were detected, while in the soil of area B there was more Ca and Mg with 3 times higher number of Mg ions. A high concentration of Ag ions was observed in both Pisoczno Lake and tap water. The severity of caries expressed by D3MFT number was 4.18 in the group aged 12-16 living in area A, and 4.24 for the inhabitants of area B. The mean value of D3MFT for the group aged 32- 45 living in area A equalled 21.58, while for area B it was 21.19. The severity of caries expressed by the mean D3MFT value in the examined group of 46-73-year-olds was 24.60 for area A, and 23.80 for area B. The observed differences were not statistically significant. CONCLUSIONS The high value of D3MFT recorded in the inhabitants of Melniki village indicates the poor condition of mineralized tooth tissues. The fact that the contents of macro-elements in the soil and drinking water increased, together with the distance from the Pisoczno Lake shoreline, did not affect the oral health of the indigenous inhabitants. No impact of silver ions contained in the lake and tap water on the condition of mineralized tooth tissue was detected in the examined group of inhabitants of Szackie Lake District.

PORÓWNANIE SKUTECZNOŚCI DEZYNFEKCJI SYSTEMU KANAŁOWEGO WYKONYWANEJ Z ZASTOSOWANIEM ORAZ BEZ STOSOWANIA KOFERDAMU

INTRODUCTION Endodontic treatment consists in necrotic pulp removal, proper root canal preparation, and sealed obliteration. The effectiveness of treatment depends on careful root canal disinfection of existing bacteria. Modern endodontics recommends the use of a rubber dam, not only to protect the patient from endodontic instrument aspiration, but also to protect root canals from bacteria existing in saliva. The aim of the study is a comparison of root canal disinfection among patients treated with and without rubber dam protection. MATERIAL AND METHODS Endodontic treatient of 36 patients who came to the Department of Conservative Dentistry and Endodontics was performed. 36 teeth were qualified to the treatment. In 17 patients the whole treatment was done with rubber dam protection. In the other 19 (II group) the treated tooth was isolated only by cotton rolls. After chamber trepanation, and after chemo-mechanical root canal preparation, samples of root canal bacteria were taken. After 24h incubation, the density of bacterial suspension was checked using a spectrophotometer. RESULTS In both groups of patients very similar results were obtained: a high density of bacterial suspension in samples taken before treatment, and a significantly lower density in samples taken after chemo-mechanical root canal preparation. CONCLUSIONS In our research the use or lack of use of a rubber dam does not change the effectiveness of root canal disinfection.