Terry O'Connor

Genotypes of predomestic horses match phenotypes painted in Paleolithic works of cave art

Archaeologists often argue whether Paleolithic works of art, cave paintings in particular, constitute reflections of the natural environment of humans at the time. They also debate the extent to which these paintings actually contain creative artistic expression, reflect the phenotypic variation of the surrounding environment, or focus on rare phenotypes. The famous paintings “The Dappled Horses of Pech-Merle,” depicting spotted horses on the walls of a cave in Pech-Merle, France, date back ∼25,000 y, but the coat pattern portrayed in these paintings is remarkably similar to a pattern known as “leopard” in modern horses. We have genotyped nine coat-color loci in 31 predomestic horses from Siberia, Eastern and Western Europe, and the Iberian Peninsula. Eighteen horses had bay coat color, seven were black, and six shared an allele associated with the leopard complex spotting (LP), representing the only spotted phenotype that has been discovered in wild, predomestic horses thus far. LP was detected in four Pleistocene and two Copper Age samples from Western and Eastern Europe, respectively. In contrast, this phenotype was absent from predomestic Siberian horses. Thus, all horse color phenotypes that seem to be distinguishable in cave paintings have now been found to exist in prehistoric horse populations, suggesting that cave paintings of this species represent remarkably realistic depictions of the animals shown. This finding lends support to hypotheses arguing that cave paintings might have contained less of a symbolic or transcendental connotation than often assumed.

Technological Analysis of the World's Earliest Shamanic Costume: A Multi-Scalar, Experimental Study of a Red Deer Headdress from the Early Holocene Site of Star Carr, North Yorkshire, UK

Shamanic belief systems represent the first form of religious practice visible within the global archaeological record. Here we report on the earliest known evidence of shamanic costume: modified red deer crania headdresses from the Early Holocene site of Star Carr (c. 11 kya). More than 90% of the examples from prehistoric Europe come from this one site, establishing it as a place of outstanding shamanistic/cosmological significance. Our work, involving a programme of experimental replication, analysis of macroscopic traces, organic residue analysis and 3D image acquisition, metrology and visualisation, represents the first attempt to understand the manufacturing processes used to create these artefacts. The results produced were unexpected—rather than being carefully crafted objects, elements of their production can only be described as expedient.

Evidence for Prehistoric Origins of Egyptian Mummification in Late Neolithic Burials

Traditional theories on ancient Egyptian mummification postulate that in the prehistoric period (i.e. the Neolithic and Chalcolithic periods, 5th and 4th millennia B.C.) bodies were naturally desiccated through the action of the hot, dry desert sand. Although molding of the body with resin-impregnated linen is believed to be an early Pharaonic forerunner to more complex processes, scientific evidence for the early use of resins in artificial mummification has until now been limited to isolated occurrences during the late Old Kingdom (c. 2200 B.C.), their use becoming more apparent during the Middle Kingdom (c. 2000-1600 BC). We examined linen wrappings from bodies in securely provenanced tombs (pit graves) in the earliest recorded ancient Egyptian cemeteries at Mostagedda in the Badari region (Upper Egypt). Our investigations of these prehistoric funerary wrappings using a combination of gas chromatography-mass spectrometry (GC-MS) and thermal desorption/pyrolysis (TD/Py)-GC-MS have identified a pine resin, an aromatic plant extract, a plant gum/sugar, a natural petroleum source, and a plant oil/animal fat in directly AMS-dated funerary wrappings. Predating the earliest scientific evidence by more than a millennium, these embalming agents constitute complex, processed recipes of the same natural products, in similar proportions, as those utilized at the zenith of Pharaonic mummification some 3,000 years later. The antibacterial properties of some of these ingredients and the localized soft-tissue preservation that they would have afforded lead us to conclude that these represent the very beginnings of experimentation that would evolve into the famous mummification practice of the Pharaonic period.

Paging through history: parchment as a reservoir of ancient DNA for next generation sequencing

Parchment represents an invaluable cultural reservoir. Retrieving an additional layer of information from these abundant, dated livestock-skins via the use of ancient DNA (aDNA) sequencing has been mooted by a number of researchers. However, prior PCR-based work has indicated that this may be challenged by cross-individual and cross-species contamination, perhaps from the bulk parchment preparation process. Here we apply next generation sequencing to two parchments of seventeenth and eighteenth century northern English provenance. Following alignment to the published sheep, goat, cow and human genomes, it is clear that the only genome displaying substantial unique homology is sheep and this species identification is confirmed by collagen peptide mass spectrometry. Only 4% of sequence reads align preferentially to a different species indicating low contamination across species. Moreover, mitochondrial DNA sequences suggest an upper bound of contamination at 5%. Over 45% of reads aligned to the sheep genome, and even this limited sequencing exercise yield 9 and 7% of each sampled sheep genome post filtering, allowing the mapping of genetic affinity to modern British sheep breeds. We conclude that parchment represents an excellent substrate for genomic analyses of historical livestock.

Livestock and deadstock in early medieval Europe from the North Sea to the Baltic

Abstract The relative abundance and mortality profiles of cattle, sheep and pigs from a series of 8th- to 11th-century sites across northern Europe are reviewed with the aim of identifying broad regional trends in livestock husbandry and redistribution. Although based on published NISP data derived from hand-collected material, the broad scale and coarse precision of the survey mitigates the worst effects of differential recovery. Marked local variation in the relative abundance of cattle and of pigs is noted in certain regions. In the latter case, the association of pigs with more easterly sites is tested and discussed. Evidence from York and its region are discussed in more detail, including an association between chalk uplands and sheep husbandry in the Middle Saxon period.

Fishing in the Adriatic at the Mesolithic–Neolithic transition: Evidence from Vela Spila, Croatia

Abstract Recent excavations at the site of Vela Spila, Korčula, on the Adriatic coast of Croatia have yielded a substantial assemblage of fish bone dating to the Mesolithic and Neolithic period. Few similar assemblages have been recovered to date, and zooarchaeological analysis of the material from Vela Spila provides an insight into the choices and practices associated with fishing across the crucial Mesolithic–Neolithic transition, and across a period of coastal change due to rising sea levels, in this area. Specialised capture and processing of mackerel (Scomber japonicus) in the Mesolithic period was indicated, with estimates indicating nearly half a tonne of mackerel processed at the site. A decrease in quantity of fish bone recovered from the Mesolithic to Neolithic phases is matched by a change in fishing strategy to opportunistic coastal fishing in the Neolithic periods. Fishing is discussed in relation to the broader lived context of the Mesolithic and Neolithic in the Adriatic, particularly its place within dietary practices and maritime activity.

Survival Analysis of Adult Tuberculosis Disease

Background We conducted a survival analysis of all the confirmed cases of Adult Tuberculosis (TB) patients treated in Cork-City, Ireland. The aim of this study was to estimate Survival time (ST), including median time of survival and to assess the association and impact of covariates (TB risk factors) to event status and ST. The outcome of the survival analysis is reported in this paper. Methods We used a retrospective cohort study research design to review data of 647 bacteriologically confirmed TB patients from the medical record of two teaching hospitals. Mean age 49 years (Range 18–112). We collected information on potential risk factors of all confirmed cases of TB treated between 2008–2012. For the survival analysis, the outcome of interest was ‘treatment failure’ or ‘death’ (whichever came first). A univariate descriptive statistics analysis was conducted using a non- parametric procedure, Kaplan -Meier (KM) method to estimate overall survival (OS), while the Cox proportional hazard model was used for the multivariate analysis to determine possible association of predictor variables and to obtain adjusted hazard ratio. P value was set at <0.05, log likelihood ratio test at >0.10. Data were analysed using SPSS version 15.0. Results There was no significant difference in the survival curves of male and female patients. (Log rank statistic  = 0.194, df  = 1, p = 0.66) and among different age group (Log rank statistic  = 1.337, df = 3, p = 0.72). The mean overall survival (OS) was 209 days (95%CI: 92–346) while the median was 51 days (95% CI: 35.7–66). The mean ST for women was 385 days (95%CI: 76.6–694) and for men was 69 days (95%CI: 48.8–88.5). Multivariate Cox regression showed that patient who had history of drug misuse had 2.2 times hazard than those who do not have drug misuse. Smokers and alcohol drinkers had hazard of 1.8 while patients born in country of high endemicity (BICHE) had hazard of 6.3 and HIV co-infection hazard was 1.2. Conclusion There was no significant difference in survival curves of male and female and among age group. Women had a higher ST compared to men. But men had a higher hazard rate compared to women. Anti-TNF, immunosuppressive medication and diabetes were found to be associated with longer ST, while alcohol, smoking, RICHE, BICHE was associated with shorter ST.

Reply to Bar-Oz and Lev-Yadun: Horse colors in time and space

The wide variation of fur and plumage colors in animals has fascinated people throughout history, and biologists have long debated its prominent role in evolution. Notably, Charles Darwin emphasized the importance of this phenotypic trait in his On the Origin of Species (first published 1859). However, although the importance of coat color in adaptation has been well documented, the underlying genetic mechanisms are less well understood.

Juliet Clutton-Brock. Animals as domesticates: a world view through history . xii+189 pages, 64 illustrations. 2012. East Lansing (MI): Michigan State University Press; 978-1-61186-028-3 hardback

past must be filled and the topic given priority. The same applies to ethnoarchaeological research focusing on sea salt collection. It is hoped that Alexianu et al.’s volume will not only be a reference collection but also a source of ideas and paradigms that will inspire further systematic research into the archaeology and ethnoarchaeology of salt-related activity by hunter-gatherers, farmers and herders.