Teruo Fukuda

Studies on the Identification of Amino Acids by Paper Electrophoresis

The identification of 19 protein composing amino acids by paper electrophoretic method was investigated.By using 5N acetic acid as the electrolyte, 19 amino acids were grouped into four groups, that is A (Lys, Arg, His, Gly, Ala), B (Val, Leu, Ileu, Ser, Thr), C (Ser, Thr, Met, Glu, Pro, Phe), D (Tyr, Cys, Asp, Try, Hypro), and the individual amino acid in these groups was identified by the further electrophoresis with 3% aqueous ammonia as the electrolyte.Amino acids of E group (Thr, Met, Phe) and F group (Tyr, Hypro), could not be separated in this case, were tried with 1% borax as the electrolyte.By the above mentioned method, the amino acids, in this study, could be separated and identified mutually, except the discrimination between Leu and Ileu, Met and Phe.

Studies on Identification of Lower Fatty Acids by Paper Electrophoresis

The paper electrophoretic method for separation and identification of monobasic n-saturated lower fatty acids (C1 to C10) was investigated. The fatty acids were separated by using 3% aqueous ammonia or the tenfold diluted of Atkins-Pantin buffer solution (pH 10) as electrolytes, and detected by staining with bromocresol green and bromothymol blue. For the electrolytes, 3% aqueous ammonia was superior to the Atkins-Pantin buffer solution in the separation of propionic and butyric acids, or caprylic and pelargonic acids.

Studies on Identification of Water soluble Coal-tar Food Colours legally permitted in Japan and the Results obtained in the Case of powdered Juices on the Market

A simple and rapid method to test water soluble coal-tar food colours legally permitted in Japan was studied using alumina column chromatography and paper electrophoresis and the following results were obtained. (1) As the buffer solution for the paper electrophoresis 5N acetic acid was most advantageous, because of inconsiderable tailing and minimized variation of relative mobilities to flavianic acid. (2) In order to identify the dyes which were difficult to identify under above mentioned condition or to increase the certainty of the identification of the other dyes, 1% borax solution was used in place of 5N acetic acid as buffer solution to give good results. (3) Mobilities of dyes in paper electrophoresis were effected with ambient temperature, but the relative mobilities to flavianic acid did not vary. (4) The application of this method to the dyes contained in powdered juices on the market gave satisfactory results, and this method could be applied to test the sample in which five colours were mixed. (5) Isolation of the dyes from the powdered juice was carried out by the alumina column chromatographic method under the mild condition, so this will be applicable to the unstable substances.