Teruo Miyagawa

Improved breast cancer detection of prone breast fluorodeoxyglucose-PET in 118 patients.

ObjectiveTo prospectively evaluate the breast cancer detection of prone breast positron emission tomography (PET) images in comparison with supine whole-body PET images. Material and methodsOne hundred and eighteen female patients (age range 28–91 years) with 122 lesions suspected of having breast cancer underwent fluorine-18 fluorodeoxyglucose PET for preoperative staging. After the whole-body image was acquired, prone breast PET imaging was performed. The findings from both images were compared with the histopathologic results. Sensitivity, specificity, positive predictive value, negative predictive value (NPV), and accuracy were used to compare the diagnostic accuracy of prone breast PET images with that of whole-body PET images. ResultsSensitivity, specificity, positive predictive value, NPV, and accuracy of whole-body PET images were 83, 50, 97, 17, and 80%, and of prone breast PET images they were 95, 50, 96, 43, and 93%. Ten of 114 breast cancerous lesions (8.8%) were detected on prone breast PET images alone. Statistical difference was found between the sensitivity, accuracy, and NPV of prone breast PET images and those of whole-body PET images (P<0.0001 for sensitivity and accuracy and P<0.0009 for NPV). ConclusionOur data about the 122 lesions, suspected of breast cancer, with regard to the usefulness of prone breast PET imaging indicate that prone breast PET images are effective in detecting breast cancer.

Degradation of deoxyribonucleic acid by guinea pig epidermal extracts.

SummaryThe capability of guinea pig epidermal extracts to hydrolyze deoxyribonucleic acid has been studied. The results of investigation by gel filtration on Sephadex G-75 column and by viscometry on the mode of hydrolysis of deoxyribonucleic acid by epidermal extracts revealed that deoxyribonucleic acid was degraded by both endonuclease and exonuclease activities. The exhaustive digestion by epidermal extracts yielded the complete degradation of deoxyribonucleic acid to mononucleosides or further metabolites. The enzyme systems involved in the deoxyribonucleic acid degradation include at least an endonuclease, an exonuclease and a phosphatase.ZusammenfassungEine Untersuchung über die DNS-Hydrolyse durch Meerschweinchen-Epidermis-Extrakt wurde durchgeführt. Gel-Filtration in der Sephadex G-75 Säule und Viskometrie ergaben eine DNS-Zerstörung durch Endonuclease- wie auch der Exonuclease-Aktivitäten. Die Verdauung zeigt eine völlige DNS-Zerstörung bis zu Mononucleosiden oder weitergehenden Metaboliten. Unter den Enzymen der DNS-Verdauung spielen zumindest eine Endonuclease, eine Exonuclease und eine Phosphatase eine Rolle.

DNA and DNA + Histone Hydrolysis by Guinea Pig Epidermal Enzymes

An acid deoxyribonuclease of guinea pig epidermis hydrolyzes chromatin DNA as well as histone-free DNA. The enzyme degrades approximately 50% of chromatin DNA, while it degrades 90% of histone-free DN