Teruo Shiro

Fermentative production of L-leucine

Mutant strains of the genera Brevibacterium and Corynebacterium having a resistance to 2-thiazolalanine or having said resistance together with a nutrient requirement, produce L-valine in a culture medium.

Bacterial Synthesis of Nucleotides: Part X. Phosphorylation of 5-Amino-4-imidazole Carboxamide RibosidePart XI. Phosphoryl Transfer between Mononucleotides and Nucleosides via A Phosphotransferase Reaction

5′-Phosphoribosyl 5-amino-4-imidazole carboxamide was prepared by incubating 5-amino-4-imidazole carboxamide riboside and a phosphate compound with the bacteria characterized to phosphorylate at C5′ via the phosphoryl transfer reaction. Aromatic phosphate compounds and 5′-nucleotides were able to act as the phosphate donor. This material was isolated chromatographically and its properties were studied. The other bacteria characterized to phosphorylate at C3′ (or 2′) also phosphorylated a little probably at C3′ (or 2′) of 5-amino-4-imidazole carboxamide riboside.The phosphoryl interconversion between nucleotides and nucleosides was studied to be carried out via the phosphoryl transfer reaction observed in bacteria. The phosphotransferase activity of Ps. trifolii mediated reversibly the phosphoryl transfer between 5′-nucleotides and nucleosides, and its optimal pH was at around 8.5, whereas that of Prot. mirabilis did transfer the phosphoryl radical from 2′- and 3′-nucleotide to nucleoside at its optimal pH...