Teruyo Fukuda

Proteomic profiling of differential display analysis for human oral squamous cell carcinoma: 14-3-3 σ Protein is upregulated in human oral squamous cell carcinoma and dependent on the differentiation level

Oral squamous cell carcinoma (OSCC) has an absolute majority of all oral cancer. We used proteomic technology to analyze the protein expression profile in OSCC tissues and accompanying surrounding normal tissues in four oral locations (buccal mucosa, gingival mucosa, oral floor, and tongue). Ten protein spots were overexpressed more strongly in cancer tissues than normal ones, and were identified as proliferating cell nuclear antigen, 14‐3‐3 ε, 14‐3‐3 σ, proteasome subunit α type 5, translationally controlled tumor protein, eukaryotic translation initiation factor 3 subunit, macrophage capping protein, and mitochondrial isocitrate dehydrogenase subunit α. Macrophage capping protein and mitochondrial isocitrate dehydrogenase subunit α had two spots. Especially, we focused on 14‐3‐3 σ protein, one of the eight identified proteins, and assessed its expression level in four oral locations of OSCC by using differential display methods. The expression level of 14‐3‐3 σ protein was upregulated in four locations of oral cavity. Eight proteins which we identified in this study may play an important role in OSCC carcinogenesis and progression and could be used as diagnostic biomarkers of OSCC.

Clinical study of terminal stage of oral cancer patiants.

口腔癌ターミナルステージ患者の臨床動態に関して免疫能, 栄養状態などの検討を行った。対象症例は口腔癌にて死亡した19例で, 男性10例, 女性9例であった。流動食 (経管, 経口含む) を中心にコントロールした症例8例, 経管栄養と中心静脈栄養を併用した者9例, 中心静脈栄養のみ2例であった。疼痛管理には解熱鎮痛剤の坐剤を用いたものが最も多く, 麻薬を用いたものはわずかに2例であった。呼吸管理の目的で気管切開を行ったもの5例で, うち1例は呼吸器による管理を行った。これらの症例について末梢血赤血球数, 白血球数, リンパ球数, リンパ球サブセット値, SCC抗原値, 血清タンパク, アルブミンについて検討を行った。