Tetsuichi Saito

Combined treatment with a β3-adrenergic receptor agonist and a muscarinic receptor antagonist inhibits detrusor overactivity induced by cold stress in spontaneously hypertensive rats

This study determined if combined treatment with the muscarinic receptor (MR) antagonist solifenacin and the β3‐adrenergic receptor (AR) agonist mirabegron could inhibit detrusor overactivity induced by cold stress in spontaneously hypertensive rats (SHRs).

Videourodyanamic Examination and Tension-free Vaginal Mesh/Transobturator Tape Surgery.

Most pelvic organ prolapse (POP) patients have lower urinary tract symptoms (LUTS) before and after POP surgery. LUTS of POP patients consist of various storage and voiding symptoms from anatomical causes. Videourodynamic examination for POP patients provides accurate information about morphological findings of the bladder and urethra, and lower urinary tract (LUT) function. The leak point pressure (LPP) measurement at cough maneuver in the standing position is important to detect urodynamic stress urinary incontinences (UDS SUI). Prolapse reduction procedure is not perfect for the detection of SUI.

MP26-04 IMPACT OF DAI-KEN-CHU-TOU ON URINARY FREQUENCY INDUCED BY COLD STRESS IN RATS

nanoparticles. Based on characterization of the ECA matrix by biomimetic analyses, this novel collagen-based biotextile may serve as a promising pelvic fascial substitute material for slings and reconstructive surgery. These findings support further experimentation of adding other nano-particles and cross-linking manufacturing steps to further enhance the balance between additional biomechanical and biocompatibility features.

MP41-05 BIOFABRICATED BONE MARROW-DERIVED CELL PATCHES RESTORE STRUCTURE AND FUNCTION OF RADIATION-INJURED URINARY BLADDERS IN RAT

groups compared with PBS group (Figure 1). Hematoxylin/eosin staining demonstrated that loss of urothelial integrity in PBS group was restored in IV and BL group. Neutrophil infiltration was also decreased in IV and BL group compared with PBS group. Histological examination revealed a significant decrease in the total number of infiltrated mast cells in IV and BL rats compared with the PBS rats (p<0.05), (Figure 2). CONCLUSIONS: This is the first study to investigate the efficacy of CHA1 for chronic IC model. Submucosal injection of CHA1 in chronic IC showed improvement of voiding pattern and histological restoration and this suggest that CHA1 might have a therapeutic potential for IC. Further studies are required to evaluate the mechanism of CHA1 treatment.

MP13-04 RISK FACTORS OF WEAK DETRUSOR FUNCTION IN BENIGN PROSTATE HYPERPLASIA PATIENTS: DETERMINING A PROGNOSTIC FACTOR FROM URODYNAMIC FINDINGS.

METHODS: 108 male volunteers with LUTS defined as International prostate symptom score over 8 were screened. Electrocardiographic signals were obtained from subjects in resting state for the analysis of their HRV. HRV parameters were analyzed to evaluate autonomic functions. We divided them into two groups by LF/HF ratio 1.6 after initial measurement. After the administration of alfuzosin 10mg once a day for 12 weeks, we evaluated HRV to investigate changes of autonomic functions. RESULTS: Total 95 LUTS patients who completed this study were enrolled. 54 subjects with LF/HF ratio under or equal 1.6 were allocated to group A and 41 patients with LF/HF ratio over 1.6 were allocated to group B. There were no statistical differences in serum PSA, volume of prostate, maximal urine flow rate between two groups. There was no statistical difference in improvement of peak urine flow rate, IPSS after treatment with alfuzosin 10mg for 12 weeks. The average LF/HF ratio of group A was increased from 0.89 0.40 to 1.79 1.80, however it was decreased from 3.93 5.47 to 1.79 1.15 in group B. CONCLUSIONS: LF/HF ratios of both groups were merged to similar values after treatments of male LUTS in this study. This study suggests that the imbalance of autonomic activity may be associated with LUTS and the efficacy of LUTS treatment.

PD70-12 IMPAIRMENT OF MICRO BLOOD FLOW IN THE BLADDER MUCOSA AND DERMIS UP-REGULATES TRPM8 CHANNELS AND INDUCE COLD STRESS RELATED FREQUENCY IN OVARIECTOMIZED RATS.

INTRODUCTION AND OBJECTIVES: Recent fMRI studies using bladder filling tasks demonstrated alterations in supraspinal LUT control networks (NT) in women with OAB, particularly regarding NT connectivity and white matter changes. It is unclear if patients with OAB show altered supraspinal responses during empty bladder conditions and if resting-state (RS) activity is altered by catheterization usually require for bladder filling tasks. We applied a functionally-motivated NT approach, using RS functional NT connectivity (RS-FNC) analysis, to examine RS related NT interactions in age-matched controls and OAB patients. Based on previous publications we hypothesize lower FNC in OAB patients. METHODS: We examined 10 healthy females (37 9y) and 10 with OAB (38 8y) and DO in urodynamics. For RS-fMRI whole brain images, using a multi-slice EPI sequence were acquired in a 3T scanner. Using SPM8, we estimated the RS NTs using the GIFT toolbox and independent component analysis (ICA) across all subjects. IC dimension estimation was performed using the minimum description length criteria, modified to account for spatial correlation. All nonneuronal ICs (e.g. cardiac-induced pulsatile artifact and head motion) were removed, resulting in a total of 9 neuronal ICs (including the default mode NT, DMN). Prior to FNC analysis, IC time courses were bandpass (0.013 Hz & 0.24 Hz). Group differences in FNC strength were calculated using the FNC toolbox for both conditions. The temporal lags between ICA-derived NTs were computed to gain directed FNC. Significant between-group FNC (and lag) results are shown at p < 0.05 FDR corrected. RESULTS: Healthy controls showed significantly higher (directed) FNC than OAB patients for: DMN (left-dominant) / DMN (right-dominant) and fronto-parietal attention NT / DMN (left-dominant). OAB patients show lower inter-networkcoupling, especially between the DMN(IC1) and the left-dominant fronto-parietal attention NT (FPN,IC4). Further, FNC differed between groups in temporal synchronicity. E.g., lag time between the DMN and FPN is shorter than between the right and left DMN in OAB patients. CONCLUSIONS: We conclude that the interplay between neuronal NTs is altered in OAB patients compared to healthy subjects already during RS. Aberrant coupling of the fronto-parietal attention NT might indicate a general neuronal deficit that impairs adequate LUT control, i.e. suppression of premature micturition reflex, consequently resulting in OAB and incontinence. These novel findings can be an important link to the underlying pathophysiology of OAB in otherwise neurological unimpaired patients.

Morphological and clinical evaluation of prostatic urethra using modified sonourethrography with retrograde jelly injection

Using modified sonourethrography (mSUG) with retrograde jelly injection to precisely measure the morphological characteristics of the prostatic urethra, we assessed prostatic urethral morphology associated with clinical parameters of benign prostatic hyperplasia (BPH).

Secondary Transobturator Tape Procedure after Combined Pelvic Organ Prolapse Repair.

A 66‐year‐old female who underwent a partial urethrectomy complained of severe incontinence due to intrinsic sphincter deficiency. Bone anchor surgical technique was performed, but in 3 years, serious pelvic organ prolapse had occurred. Consequently, anterior and posterior tension‐free vaginal mesh operation was planned. Preoperative urodynamic examination predicted postoperative stress incontinence, and concurrent transobturator tape (TOT) surgery was performed. After 3 months, stress incontinence reoccurred, and secondary TOT was performed. Relapse was probably caused by dislocation of the first TOT towards the bladder neck. Thus, the secondary TOT was placed distal to the initial tape towards the external urethral meatus, and proper tension was applied. After the operation, stress incontinence was cured. Thus, a second TOT procedure, with proper positioning and tensioning, can effectively cure stress incontinence that occurs after an initial TOT procedure.