Tetsuo Kitamura
Mitsubishi
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Publication
Featured researches published by Tetsuo Kitamura.
Journal of Pharmacological and Toxicological Methods | 2015
Keiichi Asakura; Seiji Hayashi; Atsuko Ojima; Tomohiko Taniguchi; Norimasa Miyamoto; Chiaki Nakamori; Chiho Nagasawa; Tetsuo Kitamura; Tomoharu Osada; Yayoi Honda; Chieko Kasai; Hiroyuki Ando; Yasunari Kanda; Yuko Sekino; Kohei Sawada
INTRODUCTION Multi-electrode array (MEA) systems and human induced pluripotent stem (iPS) cell-derived cardiomyocytes are frequently used to characterize the electrophysiological effects of drug candidates for the prediction of QT prolongation and proarrhythmic potential. However, the optimal experimental conditions for obtaining reliable experimental data, such as high-pass filter (HPF) frequency and cell plating density, remain to be determined. METHODS Extracellular field potentials (FPs) were recorded from iPS cell-derived cardiomyocyte sheets by using the MED64 and MEA2100 multi-electrode array systems. Effects of HPF frequency (0.1 or 1Hz) on FP duration (FPD) were assessed in the presence and absence of moxifloxacin, terfenadine, and aspirin. The influence of cell density on FP characteristics recorded through a 0.1-Hz HPF was examined. The relationship between FP and action potential (AP) was elucidated by simultaneous recording of FP and AP using a membrane potential dye. RESULTS Many of the FP waveforms recorded through a 1-Hz HPF were markedly deformed and appeared differentiated compared with those recorded through a 0.1-Hz HPF. The concentration-response curves for FPD in the presence of terfenadine reached a steady state at concentrations of 0.1 and 0.3μM when a 0.1-Hz HPF was used. In contrast, FPD decreased at a concentration of 0.3μM with a characteristic bell-shaped concentration-response curve when a 1-Hz HPF was used. The amplitude of the first and second peaks in the FP waveform increased with increasing cell plating density. The second peak of the FP waveform roughly coincided with AP signal at 50% repolarization, and the negative deflection at the second peak of the FP waveform in the presence of E-4031 corresponded to early afterdepolarization and triggered activity. DISCUSSION FP can be used to assess the QT prolongation and proarrhythmic potential of drug candidates; however, experimental conditions such as HPF frequency are important for obtaining reliable data.
Alcoholism: Clinical and Experimental Research | 2001
Hidekazu Tsukamoto; Yoshiyuki Takei; Craig J. McClain; Swati Joshi-Barve; Daniell B. Hill; Jack Schmidt; Ion V. Deaciuc; S Barve; Anna Colell; Carmen García-Ruiz; Neil Kaplowitz; José C. Fernández-Checa; Hirokazu Yokoyama; Yukishige Okamura; Yuji Nakamura; Hiromasa Ishii; Rajendar K. Chawla; Walter H. Watson; Wendy Nelson; Min Lin; Mitsuru Ohata; Kenta Motomura; Nobuyuki Enomoto; Kenichi Ikejima; Tetsuo Kitamura; Hirosumi Oide; Miyoko Hirose; Blair U. Bradford; Chantal A. Rivera; Hiroaki Kono
Archive | 1972
Tetsuo Kitamura; Akira Tamai
Journal of Pharmacological and Toxicological Methods | 2016
Masaki Honda; Akihiro Ishitomi; Yoshiko Okai; Tetsuo Kitamura; Takehito Isobe; Keiko Matsune; Seiichi Araki
Journal of Pharmacological and Toxicological Methods | 2018
Norimasa Miyamoto; Atsuko Ojima; Tetsuo Kitamura; Tomoharu Osada; Tadashi Kadowaki; Takashi Yoshinaga
Journal of Pharmacological and Toxicological Methods | 2017
Tomoharu Osada; Tetsuo Kitamura; Kimihito Yoshikawa; Fumihide Bunai; Mayumi Obo; Hideomi Uchida; Yasuyuki Onishi; Hideaki Hiratsuka; Hiroaki Inoue
Journal of Pharmacological and Toxicological Methods | 2014
Mototsugu Sakakibara; Tetsuo Kitamura; Makoto Otsuki; Fumihide Bunai; Daisuke Minami; Yukiko Fujisaki; Masaru Sekijima; Tomoharu Osada
Journal of Pharmacological and Toxicological Methods | 2011
Mototsugu Sakakibara; Mikiko Koike; Tetsuo Kitamura; Minoru Tateno; Tomoharu Osada; Fumimasa Nomura; Tomoyuki Kaneko; Masaru Sekijima; Kenji Yasuda
Journal of Pharmacological and Toxicological Methods | 2010
Atsushi Sugiyama; Tetsuo Kitamura; Fumimasa Nomura; Tomoyuki Kaneko; Yuji Nakamura; Peter Sartipy; Kenji Yasuda
Biophysical Journal | 2010
Fumimasa Nomura; Tetsuo Kitamura; Tomoyuki Kaneko; Kenji Yasuda