Tetsushi Yamada

Enlargement of mandibular canal without hypesthesia caused by extranodal non-Hodgkin’s lymphomaA case report

A rare condition of enlargement of the mandibular canal caused by an extra-nodal non-Hodgkins lymphoma in a 59-year-old Japanese woman was reported. The patient had a swelling of the hard palate and protrusion of both ocular bulbs, which had been present for 10 years. A panoramic radiograph revealed that the right mandibular canal was widely enlarged, extending from the mandibular foramen to the mental foramen, without bone destruction. The continuous dilation of the mandibular canal to an approximate 15-mm width was associated with peripheral bony sclerosis. Computed tomography and magnetic resonance imaging showed a soft tissue tumor inside the mandibular canal. The lesion demonstrated expansive growth in the orbits, extending to the skull base through the superior orbital fissures and cavernous sinus. The lymphoma was suspected to have grown so slowly that the adjacent mandibular canal and ocular bulbs enlarged without destroying the normal bone and nervous tissue.

Fluorescent analysis of 5-aminolevulinic acid-induced protoporphyrin-IX in mouse transplanted tumor tissues

Abstract This study investigates the optimal method of administrating 5-aminolevulinic acid (ALA) in the context of photodynamic therapy (PDT) by analyzing protoporphyrin-IX (Pp-IX) fluorescence in skin and tongue tumor tissues. For skin tumor, Pp-IX intensities in nude mouse skin cancer were measured after topical application of three photosensitizers: ALA, ALA-methyl or ALA-hexyl derivatives. The combined effects of ultrasound and/or depilation cream were also examined. For the tongue tumor, Pp-IX intensities in mouse tongue cancer were compared with those in the normal tongue after intraperitoneal, oral or topical administration of ALA. The results showed the effects of a combination of depilation cream in all photosensitizers in the skin tumor. Application of hexyl derivative in combination with ultrasound or depilation cream showed higher Pp-IX concentration in the tumor tissue. Maximum Pp-IX accumulation in the tongue tumor tissue was seen at 5 h after oral administration of ALA. In contrast, the topical administration of 20% ALA cream showed lowest Pp-IX accumulation in the tongue tumor throughout the experiments.

Imaging of the atheroma in a frozen‐section of human atheroscleotic tissue by FT‐IR microscopy

There is a long history of standard H. & E. staining for pathological diagnostic samples, where the organism is fixed with formaldehyde. However, one should not be neglect that the pathological diagnostic sample has an artificial, secondary protein structure, differing from the structure of just frozen‐sectioned organism. We observed quantitatively the cholesterol distribution in the atheroma of a frozen‐section of autopsied aorta by imaging, for the first time, using the Fourier transform infrared microscopy (FT‐IRM) technique. We detected the cholesterol distribution and found that the protein structure changed to a β‐sheet conformation, from an α‐helix conformation on the inside edge of the calcified area.