Thangavel Sivakumar

UV Spectrophotometric Method for the Estimation of Valacyclovir HCl in Tablet Dosage Form

A simple, sensitive, highly accurate UV spectrophotometric method has been developed for the determination of valacyclovir in bulk and tablet dosage form. Solution of valacyclovir in 0.1N HCl shows maximum absorbance at 255 nm. Beer’s law was obeyed in the concentration range of 5-25 mcg mL-1 with 1.0910x104 mol-1 cm-1, the slope, intercept, correlation coefficient, detection and quantitation limits were also calculated. The proposed method has been applied successfully for the analysis of the drug in pure and in its tablets dosage forms. Result of percentage recovery and placebo interference shows that the method was not affected by the presence of common excipients. The percentages assay of valacyclovir HCl in tablet was 99.82%. The method was validated by determining its sensitivity, accuracy and precision which proves suitability of the developed method for the routine estimation of valacyclovir in bulk and solid dosage form.

Anti-pyretic and anti-inflammatory activity of chloroform extract of Croton roxburghii in standard animal models

The chloroform extract of Croton roxburghii (Family: Euphorbiaceae) was evaluated for its antipyretic effects in Brewers yeast induced hyperthermia in rats. The anti-inflammatory effect of the Croton roxburghii was also evaluated by using carrageenan, dextran, histamine, serotonin induced rat paw oedema and cotton pellet induced granuloma (chronic) models in rats. The chloroform extract of Croton roxburghii (CECR) exhibited significant anti-pyretic and anti-inflammatory effect at the dose 50, 100 and 200 mg/kg. Maximum inhibition (55.32%) was notedat the dose of 200 mg/kg after 3 h of drug treatment in carrageenan induced paw oedema, whereas the Indomethacin (standard drug) produced 61.33% of inhibition. The extract exhibited significant anti-inflammatory activity in dextran induced paw edema in a dose dependent manner. In the chronic model (cotton pellet induced granuloma) the CECR (200 mg/kg) and Indomethacin (10 mg/kg) showed decreased formation of granuloma tissue by 52.32% and 56.32% respectively. The extract also exhibited a significant antipyretic response in Brewers yeast induced pyrexia in rats. Thus, the present study revealed that the CECR exhibited significant antipyretic and anti-inflammatory activity in the tested animal models.