Theodore I. Malinin

Unusual synovial fluid findings in Reiter's syndrome.

Excerpt Reiters syndrome is recognized as the symptom complex of nongonococcal urethritis, conjunctivitis, arthritis, and, frequently, mucocutaneous lesions. The clinical aspects of the syndrome h...

Protection of lethally irradiated guinea pigs with fresh and frozen homologous peripheral blood leukocytes.

Abstract Lethally irradiated (550 r Co 60 ) Princeton guinea pigs can survive beyond a 30-day period following the early postirradiation infusion of adequate numbers of either fresh (1 × 10 8 ) or frozen (1 × 10 9 ) homologous peripheral blood leukocytes. Animals infused with larger numbers of fresh homologous cells 24 hr following radiation died, as did all control animals. Animals surviving the lethal period failed to demonstrate any gross evidence of homologous disease as late as 272 days following irradiation. A histopathological study of the surviving animals and animals sacrificed at daily intervals during the postirradiation period (the subject of a separate report) suggests that the infused cells provided a necessary transient mechanism for combating bacterial infection.

The Clinical Significance of Deoxyribonucleic Acid Particles in Synovial Fluid

Excerpt Examination of synovial fluid has proved to be of definite value in the diagnosis of certain rheumatic conditions; but more importantly, it has led to greater understanding of the pathogene...

Effect of dimethyl sulfoxide on cooling rates of unrestrained rats

Summary A total of 170 adult rats were exposed to 1°C, 15°C, and 25°C environments following ip injections of 3 to 6 g per kg of DMSO in saline. Shaved subjects receiving 3 g per kg of DMSO in a 1°C environment cooled to a body temperature of 10°C within 239 min; subjects receiving 6 g per kg of DMSO cooled to 10°C in 175 min. Shaved saline controls required 412 min to reach the same temperatures. Unshaved rats in a 1°C environment which were treated with 6 g per kg of DMSO cooled to 10°C within 383 min. Unshaved saline controls failed to become hypothermic. Shaved subjects injected with 6 g per kg of DMSO in an environment of 15°C gave a mixed response, a few maintaining normothermia, some cooling a few degrees and then rewarming, and 50% cooling to environmental temperatures. All controls remained normothermic. At an environmental temperature of 25°C, DMSO-treated animals dropped 3.6°C and then rewarmed. Control subjects remained normothermic. Each DMSO-treated group, therefore, manifested a significant disturbance of temperature regulation.

Production of interleukin-1β by periodic acid-oxidized human peripheral blood mononuclear cells

Interleukin-1 (IL-1) production by periodic acid (H5IO6)-oxidized human peripheral blood mononuclear (PBMN) cells was assessed by the thymocyte co-mitogenesis assay. Maximum IL-1 levels (∼ 1.2 U/ml) in the conditioned media of PBMN cells were registered within the first 24 hrs post-oxidation, whereas no IL-1 was detected in the media from 24 hrs control cultures. Thymocyte proliferation, driven by periodic acid-induced IL-1, was abolished by an antibody to IL-1alpha and IL-1β. Quantitative analysis of IL-1-containing medium by radioimmunoassay (RIA) indicated that IL-1β comprised about 80% of total IL-1. Partial characterization of H5IO6-induced IL-1β indicated that it was identical to IL-1 produced by lipopolysaccharide-stimulated macrophages. It is concluded that oxidation of human PBMN cells by H5IO6 triggers synthesis and release of IL-1, most of which was in its IL-1β form.

Evaluation of renal function in perfused rabbit kidneys

Extractions of inulin and para-aminohippurate were measured in perfused rabbit kidneys. These tests, which can be performed with relative rapidity, give an indication of a functional state of perfused kidney. It is suggested that these procedures could be used to determine in vitro “transplantability potential” of donor kidneys.

Cytometric and electron microscopic studies of the direct interaction of divalent nickel with intact and chemically modified HuT-78 lymphoblasts

Cytometric and ultrastructural studies on 24 hr cultures of intact, 1.0 mM H5I06, and 0.1 mM SeO2-oxidized HuT-78 lymphoblasts were performed after their direct, 30 min interaction with 1.0 mM NiCl2. Except for moderately depressed cell viability, divalent nickel did not alter the progression of intact and oxidized target cells through the phases of the cell cycle.Although the plasma membrane remained structurally intact, marked distortion of mitochondria structure and increased osmiophilia were an invariable attribute of all nickel-pulsed cells. Moreover, numerous electron-opaque, intracellular depositions were detected in SeO2-oxidized, nickel-pulsed cells. It is concluded that the initial state of plasma membrane, and the interaction of nickel with other trace elements, have jointly determined the response of HuT-78 cells to brief and direct, divalent nickel pulses.

Clinical Value of Systematic Examination of Synovial Fluid.

Excerpt Synovial fluid analysis is recognized as an important part of the diagnostic work-up of the patient with arthritis. For the past 3 years we have examined several hundred synovial fluids fro...