Thiago A. Moraes

Spontaneous ultra-weak light emissions from wheat seedlings are rhythmic and synchronized with the time profile of the local gravimetric tide

Semi-circadian rhythms of spontaneous photon emission from wheat seedlings germinated and grown in a constant environment (darkened chamber) were found to be synchronized with the rhythm of the local gravimetric (lunisolar) tidal acceleration. Time courses of the photon-count curves were also found to match the growth velocity profile of the seedlings. Pair-wise analyses of the data—growth, photon count, and tidal—by local tracking correlation always revealed significant coefficients (P > 0.7) for more than 80% of any of the time periods considered. Using fast Fourier transform, the photon-count data revealed periodic components similar to those of the gravimetric tide. Time courses of biophoton emissions would appear to be an additional, useful, and innovative tool in both chronobiological and biophysical studies.

Coincidence of biophoton emission by wheat seedlings during simultaneous, transcontinental germination tests

Measurements of spontaneous ultra-weak light (biophoton) emission from native Brazilian and German wheat seedlings in three simultaneous series of germination tests are presented, two run in Germany and one in Brazil. Seedlings in both countries presented semi-circadian rhythms of emission that were in accordance with the local lunisolar gravimetric tidal acceleration, as did seeds which had been transported from Brazil to Germany. The simultaneity of the photon emission patterns in all tests argues for the lunisolar tide and its rhythmic variations as regulators of the natural rhythm of photon emission. However, seedlings from seed samples transported from Brazil to Germany showed, in addition, a temporary disturbance within the emission periodicity which may indicate a possible short-term acclimatization to the new location.

Lunisolar tidal synchronism with biophoton emission during intercontinental wheat-seedling germination tests

Synchronic measurements of spontaneous ultra-weak light emission from germinating wheat seedlings both in Brazil and after transportation to Japan, and with a simultaneous series of germinations with local seedlings in the Czech Republic, are presented. A series of tests was also performed with samples returned from Japan to Brazil and results compared with those from undisturbed Brazilian seedlings. Native seedlings presented semi-circadian rhythms of emission which correlated with the gravimetric tidal acceleration at their locality, as did seeds which had been transported from Brazil to Japan, and then returned to Brazil. Here, however, there were very small disturbances within the periodicity of emissions, perhaps as a result of similar tidal profiles at locations whose longitudes are 180° apart, as in this case, different from previous results obtained in Brazil–Germany tests with other longitude shift. This feature of the Brazil and Japan locations may have minimized the requirement for the acclimatization of the transported seed to their new location.

Using multifractal analysis of ultra-weak photon emission from germinating wheat seedlings to differentiate between two grades of intoxication with potassium dichromate

The aim of the present study was to test whether the multifractal properties of ultra-weak photon emission (UPE) from germinating wheat seedlings (Triticum aestivum) change when the seedlings are treated with different concentrations of the toxin potassium dichromate (PD). To this end, UPE was measured (50 seedlings in one Petri dish, duration: approx. 16.6- 28 h) from samples of three groups: (i) control (group C, N = 9), (ii) treated with 25 ppm of PD (group G25, N = 32), and (iii) treated with 150 ppm of PD (group G150, N = 23). For the multifractal analysis, the following steps where performed: (i) each UPE time series was trimmed to a final length of 1000 min; (ii) each UPE time series was filtered, linear detrended and normalized; (iii) the multifractal spectrum (f(α)) was calculated for every UPE time series using the backward multifractal detrended moving average (MFDMA) method; (iv) each multifractal spectrum was characterized by calculating the mode (αmode) of the spectrum and the degree of multifractality (Δα); (v) for every UPE time series its mean, skewness and kurtosis were also calculated; finally (vi) all obtained parameters where analyzed to determine their ability to differentiate between the three groups. This was based on Fishers discriminant ratio (FDR), which was calculated for each parameter combination. Additionally, a non-parametric test was used to test whether the parameter values are significantly different or not. The analysis showed that when comparing all the three groups, FDR had the highest values for the multifractal parameters (αmode, Δα). Furthermore, the differences in these parameters between the groups were statistically significant (p < 0.05). The classical parameters (mean, skewness and kurtosis) had lower FDR values than the multifractal parameters in all cases and showed no significant difference between the groups (except for the skewness between group C and G150). In conclusion, multifractal analysis enables changes in UPE time series to be detected even when they are hidden for normal linear signal analysis methods. The analysis of changes in the multifractal properties might be a basis to design a classification system enabling the intoxication of cell cultures to be quantified based on UPE measurements.

Spontaneous Light Emission of Wheat Seedlings With K2Cr2O7

It is analyzed the spontaneous light emission of germinating seeds, in tests with optimum and stressed conditions; the growth performance is correlated to the total photon-count, with pronounced peak for low stress (<100ppm Dichromate) solutions.

Periodic Time-Components in Spontaneous Ultra-Weak Photon Emission of Wheat Seedlings in Stressing Solutions

Total photon-counts of seedlings under stress show an inverse relation to the solution concentration, as well as the amplitude of periodic time components (multi-sine fitting), with evidence of biorhythm fundamental period T~6h.

Rhythmicities in the spontaneous photon emission of wheat seedlings

The photon-counts of two series of wheat seeds germinating in distilled water are studied in respect to germination efficiency and spontaneous photon (biophoton) emission, with clear indications of day- and month-like patterns in the analyzed data.

Germination Capability of Wheat Seeds in Correlation with Delayed Luminescence Intensity

The wheat seeds’ delayed luminescence is related to the samples´ germination rate, with inverse relation observed and more clearly evidenced at the end of measurement term (4<t<5s) for all paired groups.

Spontaneous light emission of wheat seedlings in leachate solutions

The photon-counts of two series of wheat seedlings growing in landfills leachate solutions are studied in respect to germination efficiency and spontaneous photon (biophoton) emission, evidencing the relation between biophotonic emission and the seedlings development and also the its variability.