Thiago Pires Anacleto

Studies of distribution and recurrence of Helicobacter spp. gastric mucosa of dogs after triple therapy

Purpose: To analyze the triple antimicrobial therapy in positive Helicobacter spp. dogs and to investigate recurrence. Methods: A total of 20 dogs underwent endoscopy followed by gastric biopsy using the rapid urease test and histopathology stained with Giemsa. Ten animals were treated with triple therapy recommended for humans and divided into control and experimental group. The control group was kept in isolation while the experimental group was placed in contact with positive animals during 60 days. Results: The prevalence of infection in animals in this experiment was 100%, and more frequent in the fundus and the gastric body. Therapy for 7 days using clarithromycin, amoxicillin and lansoprazole was effective in 100% of the animals. Recurrence of the infection in 80% of dogs in the experimental group, while the control group remained eradicated after 60 days. Conclusion: Crowded environments associated with close contact with dogs infected with helicobacter are a determinant for transmission of Helicobacter spp. between canines. Key works: Helicobacter infections. Endoscopy. Gastric Mucosa. Dogs. RESUMO Objetivo: Avaliar a eficacia da terapia triplice em caes naturalmente infectados pelo Helicobacter spp. e investigar a recorrencia da infeccao pelo contato com animais infectados. Metodos: Foram utilizados 20 caes, submetidos a endoscopia digestiva alta seguida de biopsia gastrica usando teste rapido da urease e histopatologia corada pelo Giemsa. Dez animais foram tratados com terapia triplice preconizada para humanos e divididos em grupo controle e experimental. O grupo controle foi mantido em isolamento enquanto que o grupo experimento foi colocado em contato com os animais positivos durante 60 dias. Resultados: A prevalencia da infeccao nos animais deste experimento foi de 100%, e mais frequente no fundo e corpo gastrico. A terapia durante 7 dias empregando claritromicina, amoxicilina e lansoprazol foi eficaz em 100% dos animais. Houve recorrencia da infeccao em 80% dos caes do grupo experimental, enquanto que o grupo controle manteve-se erradicado apos 60 dias. Conclusao: Ambientes aglomerados associado a intimo contato com caes infectados por helicobacterias e fator determinante para transmissao do Helicobacter spp. entre caninos. Descritores: Infeccao por Helicobacter . Endoscopia. Mucosa Gastrica. Caes.PURPOSE To analyze the triple antimicrobial therapy in positive Helicobacter spp. dogs and to investigate recurrence. METHODS A total of 20 dogs underwent endoscopy followed by gastric biopsy using the rapid urease test and histopathology stained with Giemsa. Ten animals were treated with triple therapy recommended for humans and divided into control and experimental group. The control group was kept in isolation while the experimental group was placed in contact with positive animals during 60 days. RESULTS The prevalence of infection in animals in this experiment was 100%, and more frequent in the fundus and the gastric body. Therapy for 7 days using clarithromycin, amoxicillin and lansoprazole was effective in 100% of the animals. Recurrence of the infection in 80% of dogs in the experimental group, while the control group remained eradicated after 60 days. CONCLUSION Crowded environments associated with close contact with dogs infected with helicobacter are a determinant for transmission of Helicobacter spp. between canines.

Histological aspects of autologous transplantation of different fragments of the spleen in rats

PURPOSE To evaluate macro and microscopically the evolution of autotransplants of fragments of spleen different fragments in the greater omentum, after eight weeks of observation. METHODS Twenty rats Wistar were used, males and adults, submitted to total splenectomy and divided in two groups. The group I - ten animals with implant of spleen fragment (25% weight of spleen) in the omentum; and group II - ten animals with implant of spleen fragment (30% weight of spleen) in the omentum. It was analyzed macro and microscopically the evolution of the implant. RESULTS It was observed adherences to the adjacent tissues and vascularization in all of the fragments transplanted. The group I and II presented white pulp with follicular formations and lymphoid tissue preserved, and the red pulp in cordon aspect. The group II presented white pulp more disorganized and red pulp hemorrhagic. The active macrophages were observed in the group I and II. CONCLUSION The splenic autotransplantation of the group I showed better regeneration.