Thierry Lamaze

Complementarity in mineral nitrogen use among dominant plant species in a subalpine community

The underlying mechanisms that enable plant species to coexist are poorly understood. Complementarity in resource use is among the major mechanisms proposed that could favor species coexistence but is insufficiently documented. In alpine soil, low temperatures are a major constraint for the supply of plant nitrogen. We carried out (15)N labeling of soil mineral N to determine to what extent four major species of a subalpine community compete for N, or develop ionic (NH(4)(+) vs. NO(3)(-)) or temporal complementarity. The Poaceae took up much more (15)N per soil area unit than the ericaceous species, and all species displayed three major strategies in exploiting (15)N: (1) uptake mainly early in the growing season (Vaccinium myrtillus), (2) uptake at a slow and similar rate throughout the growing season (Rhododendron ferrugineum), and (3) uptake at high rates over the growing season (Festuca eskia and Nardus stricta). However, while F. eskia used (15)NH(4)(+) mainly early and (15)NO(3)(-) mainly late in the growing season, the reverse was observed for N. stricta. Taking into account (15)N dilution in soil NH(4)(+) and NO(3)(-) pools, we calculated that NH(4)(+) provided more than 80% of the mineral N uptake in Ericaceae and about 60% in grasses. Together, such ionic and temporal complementarity would reduce competition between species and could be a major mechanism promoting species diversity.

Endogenous sink-source interactions and soil nitrogen regulate leaf life-span in an evergreen shrub

How the balance between exogenous and endogenous nitrogen for shoot growth varies with soil nitrogen availability, and its consequences on leaf life-span, have rarely been studied within a single species in the field. In this study, we investigated two Rhododendron ferrugineum populations with contrasting leaf life-span. Soil nitrogen availability and nitrogen resorption of different leaf age classes were assessed, as were the interactions between plant compartments, using (15)N labelling and sink organ suppression. The population growing on poorer soil had a shorter leaf life-span (17.9 vs 21.5 months) and a higher net contribution of leaf reserves to shoot growth (32% vs 15%), achieved by faster nitrogen resorption and greater shedding of young nitrogen-rich leaves. For both populations, wood contributed to over 40% of shoot nitrogen demand. Both the negative relationship between current-year shoot mass and the percentage of 1-yr-old attached leaves and the delay of leaf shedding after bud removal suggest that shoot development has a strong effect on leaf life-span. Our results suggest that, contrary to the evolutionary response, plastic response to low soil nitrogen could reduce leaf life-span in evergreen plants. In addition, leaf life-span seems to be strongly influenced by the discrepancy between shoot nitrogen demand and soil nitrogen uptake rather than nitrogen demand alone.

Leaf life span optimizes annual biomass production rather than plant photosynthetic capacity in an evergreen shrub.

SUMMARY *Owing to nitrogen (N) translocation towards new leaves, the shedding of old leaves can increase the whole-plant carbon gain. It occurs when their photosynthetic nitrogen use efficiency (PNUE) declines below a given threshold. *Here, we investigated variations in net photosynthetic capacity (A(max)), N resorption and PNUE in populations of Rhododendron ferrugineum presenting different mean leaf life spans (LLS). *Both populations had comparable annual leaf surface area production and A(max) across leaf-age cohorts. Branch photosynthetic capacity was up to 95% higher in the population with the longer LLS mainly because of the high contribution of old leaves to the total leaf area. Despite lower N concentrations, old leaves maintained relatively high A(max) and consequently PNUE that were higher than or similar to the values found in current-year leaves. *As the ratio of PNUE in old to PNUE in new leaves was always higher than the fraction of leaf N resorbed during leaf shedding, we concluded that leaf shedding did not improve plant photosynthetic capacity. We suggest that in R. ferrugineum, leaf shedding is mainly controlled by the leaf storage function and, therefore, that models aiming to explain LLS should not only consider the leaf carbon assimilation function, particularly in nutrient-poor habitats.

Subalpine Pyrenees received higher nitrogen deposition than predicted by EMEP and CHIMERE chemistry-transport models

Deposition of reactive nitrogen (N) from the atmosphere is expected to be the third greatest driver of biodiversity loss by the year 2100. Chemistry-transport models are essential tools to estimate spatially explicit N deposition but the reliability of their predictions remained to be validated in mountains. We measured N deposition and air concentration over the subalpine Pyrenees. N deposition was found to range from 797 to 1,463 mg N m−2 year−1. These values were higher than expected from model predictions, especially for nitrate, which exceeded the estimations of EMEP by a factor of 2.6 and CHIMERE by 3.6. Our observations also displayed a reversed reduced-to-oxidized ratio in N deposition compared with model predictions. The results highlight that the subalpine Pyrenees are exposed to higher levels of N deposition than expected according to standard predictions and that these levels exceed currently recognized critical loads for most high-elevation habitats. Our study reveals a need to improve the evaluation of N deposition in mountains which are home to a substantial and original part of the world’s biodiversity.

Complex interactions between a legume and two grasses in a subalpine meadow

Interactions between plants are a complex combination of positive and negative interactions, with the net outcome depending on environmental contexts. The more frequent association of Trifolium alpinum (legume) with Festuca eskia than with Nardus stricta (grasses) in many Pyrenean subalpine meadows suggests a differential ability to use nitrogen (N) derived from N(2) fixation. In the field, we investigated the interactions between the legume and grasses and, in the glasshouse, the transfer of (15)N from the legume to the grasses. In one grass-Trifolium mixture, the legume had a strong positive effect on the biomass and N content of the grass as compared to pure grass stands. When both grasses grew together with the legume, only Festuca benefited from the presence of Trifolium but, surprisingly, the benefit decreased with increasing Trifolium abundance. Leaf labeling experiments with (15)N-NH(4)(+) revealed a higher transfer of (15)N from Trifolium to Festuca than to Nardus, suggesting a more direct N pathway between the two species. This more direct pathway could prevent Nardus from benefiting from the legume N in the three-species mixtures. Thus, the positive interactions between N-fixers and nonfixers appear to be largely species-specific and to depend strongly on the species in the plant assemblage.

Activities of peroxidase (soluble and cell wall bound) and of other H2O2 scavenging enzymes are markers of the flower bud development stage in lemon

Specific activities of H 2 O 2 scavenging enzymes, soluble peroxidase (G-POD), catalase (CAT) and ascorbate peroxidase (APX), and cell wall bound (CWB) G-POD, have been characterized in flower buds and leaves in potted lemon trees ( Citrus limon L. Burm.) growing in a glasshouse in Tunisia. In leaves during the winter rest period (from September to December), enzyme activities did not change markedly. Then, leaf G-POD (soluble and CWB activity) increased 4-5 fold in January as the buds reached dormancy release. In growing buds, the activities of the three enzymes increased strongly (10-100-fold) between dormancy and full bloom and decreased slightly (3-fold) post-bloom (beginning of fruit-set). Along the shoots, the developmental stages of flower buds advanced from node 1 to node 5. G-POD activity increased from the shoot apex toward the base of the twig in both leaves and buds. A similar trend was observed for APX and CAT but their activities tended to decrease slightly far from the apex. Retardation of flowering by application of growth regulators strongly reduced leaf-specific activities of the H 2 O 2 scavenging enzymes. Therefore, G-POD activity (APX and to a lesser extent CAT) appears to be a good marker of flower formation in lemon (from bud swelling to full bloom). The results also suggest a link between H 2 O 2 production and flower bud development.

Genetic Analysis of the Transpiration Control in Sunflower (Helianthus Annuus L) Subjected to Drought

Stomatal control of transpiration was implied as the major strategies by which plants cope with water stress. Here we did investigate the genetic control of this process using the following trait: Fraction of Transpirable Soil Water threshold (FTSWt) representing the threshold of soil water content at which the stomatal control of transpiration started. We conducted a progressive water deficit experiment using recombinant inbred lines (RILs) of sunflower and we analyzed the variation of FTSWt. Quantitative trait loci (QTL) mapping was then performed to determine the loci involved and to identify the genetic control. This work has shown, for the first time, QTL mapping for FTSWt in crops. In this work QTL mapping was made in sunflower.