Thomas A. Fleisher
George Washington University
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Featured researches published by Thomas A. Fleisher.
Cytokine | 1993
Glenn H. Bock; Clarisa Long; Monica L. Riley; Jeffrey D. White; Carole C. Kurman; Thomas A. Fleisher; Maria Tsokos; Margaret Brown; Debbie Serbousek; William D. Schwietermann; David L. Nelson
We have established a cell line (DS-1) of B-cell lineage in long-term culture. It was derived from an immunodeficient patient with intestinal lymphangiectasia and lymphoma by culturing malignant pleural effusion cells with IL-6 in vitro. The cell surface phenotype was; PCA-1, HLA Class II(+); CD25, CD19, CD20, CD30, CD38(-). Cell proliferation was poor in medium and exhibited an eight-fold, dose-dependent increase of proliferation in response to rIL-6 of human but not murine origin. The secretion of IgG into culture supernatants by DS-1 was not enhanced by rIL-6. While constitutive production of IL-6 was not detected by bioassay using murine B9 hybridoma cells or by ELISA, the presence of IL-6 message was detected in polyA+ selected mRNA by Northern analysis. Spontaneous proliferation of DS-1 cells was inhibited by neutralizing polyclonal antibodies to IL-6 (37%) and mAb to IL-6 (54%) and IL-6R (53%). DS-1 expressed both high and low affinity IL-6 receptors (Kd 1.2 x 10(-11) and 6.7 x 10(-10), respectively) by radiolabelled binding and Scatchard analysis. Thus, DS-1 represents an autocrine IL-6-producing cell line of B-cell lineage which resembles lymphoid malignancies arising in patients with AIDS and other immunodeficiency diseases. Despite constitutive IL-6 production, the in vitro growth of DS-1 is dependent upon exogenous IL-6. DS-1 may thus be useful as a model of IL-6 dependency. This cell line may also facilitate development of strategies for diagnosis and treatment of B-cell lymphomas in immunocompromised patients.
Archive | 1994
Glenn H. Bock; David L. Nelson; Carole C. Kurman; Thomas A. Fleisher
Archive | 1998
Steven M. Holland; Susan E. Dorman; Annette Kwon; Ian F. Pitha-Rowe; David M. Frucht; Susan M. Gerstberger; Gary J. Noel; Per Vesterhus; Margaret R. Brown; Thomas A. Fleisher
Archive | 2014
Pamela Stratton; Edward W. Cowen; Douglas B. Kuhns; Harry L. Malech; Philip M. Murphy; Rosamma DeCastro; George Grimes; Samuel T. Hwang; Stefania Pittaluga; Katherine R. Calvo; Robert L. Giuntoli; Moses O. Evbuomwan; Patricia Littel; Martha Marquesen; Dianne Hilligoss; Judy Starling; Thomas A. Fleisher; Debra A. Long Priel; Melissa Merideth; David H. McDermott; Qian Liu; Daniel Velez; Jean Ulrick
Archive | 2013
Michael J. Lenardo; Jennifer M. Puck; Thomas A. Fleisher; Jack Bleesing; Margaret R. Brown; Stephen E. Straus; Janet K. Dale; Richard M. Siegel
Archive | 2013
Rebecca H. Buckley; Zuhair K. Ballas; Mark Ballow; R. Michael Blaese; Francisco A. Bonilla; Mary Ellen Conley; Charlotte Cunningham-Rundles; Alexandra H. Filipovich; Thomas A. Fleisher; Ramsay L. Fuleihan; Erwin W. Gelfand
Archive | 2013
Peter E. Lipsky; Harry L. Malech; Thomas A. Fleisher; Sebastian Brenner; Richard M. Siegel; Mitchell E. Horwitz; Margaret R. Brown; Cynthia Martinez; Sule Yavuz; Jack Bleesing; Margarida M. Souto-Carneiro
Archive | 2013
Shepherd H. Schurman; Thomas A. Fleisher; Susan F. Leitman; R M Blaese; Elizabeth Garabedian; Margaret Brown; Wendy Bernstein; Sherry Lau Soenen; G J Jagadeesh; William J. Ramsey
Archive | 2013
Ian Magrath; Leonard H. Wexler; Dimiter S. Dimitrov; Ronald E. Gress; Crystal L. Mackall; Thomas A. Fleisher; Margaret R. Brown; Mary P. Andrich; Clara C. Chen
Archive | 2012
V. Koneti Rao; Jennifer M. Puck; Elaine S. Jaffe; Stefania Pittaluga; Jeffrey I. Cohen; Thomas A. Fleisher; Kennichi C. Dowdell; Julie E. Niemela; Joie Davis; Ronald L. Hornung; João Bosco