Thomas B. Moorman

Denitrification in Wood Chip Bioreactors at Different Water Flows

Subsurface drainage in agricultural watersheds exports a large quantity of nitrate-nitrogen (NO(3)-N) and concentrations frequently exceed 10 mg L(-1). A laboratory column study was conducted to investigate the ability of a wood chip bioreactor to promote denitrification under mean water flow rates of 2.9, 6.6, 8.7 and 13.6 cm d(-1) which are representative of flows entering subsurface drainage tiles. Columns were packed with wood chips and inoculated with a small amount of oxidized till and incubated at 10 degrees C. Silicone sampling cells at the effluent ports were used for N(2)O sampling. (15)Nitrate was added to dosing water at 50 mg L(-1) and effluent was collected and analyzed for NO(3)-N, NH(4)-N, and dissolved organic carbon. Mean NO(3)-N concentrations in the effluent were 0.0, 18.5, 24.2, and 35.3 mg L(-1) for the flow rates 2.9, 6.6, 8.7, and 13.6 cm d(-1), respectively, which correspond to 100, 64, 52, and 30% efficiency of removal. The NO(3)-N removal rates per gram of wood increased with increasing flow rates. Denitrification was found to be the dominant NO(3)-N removal mechanism as immobilization of (15)NO(3)-N was negligible compared with the quantity of (15)NO(3)-N removed. Nitrous oxide production from the columns ranged from 0.003 to 0.028% of the N denitrified, indicating that complete denitrification generally occurred. Based on these observations, wood chip bioreactors may be successful at removing significant quantities of NO(3)-N, and reducing NO(3)-N concentration from water moving to subsurface drainage at flow rates observed in central Iowa subsoil.

Biodegradation of imidacloprid by an isolated soil microorganism.

Imidacloprid (1-[(6-chloro-3-pyridinyl)-methyl]-N-nitro-2-imidazolidinimine), a chloronicotinyl insecticide used to control biting and sucking insects, is very persistent in the soil with a half-life often greater than 100 days. Although a few soil metabolites have been reported in the literature, there are no reports of imidacloprid-degrading soil microorganisms. Our objectives were to discover, isolate, and characterize microorganisms capable of degrading imidacloprid in soil. Two soil-free stable enrichment cultures in N-limited media were obtained that degraded 19 mg L− 1 (43%) and 11 mg L− 1 (16%) of the applied imidacloprid, and produced about 19 mg L− 1 6-chloronicotinic acid in three weeks. Enrichment media without microorganisms had no loss of imidacloprid. Strain PC-21, obtained from the enrichment cultures, degraded 37% to 58% of 25 mg L− 1 imidacloprid in tryptic soy broth containing 1 g L− 1 succinate and D-glucose at 27°C incubation over a period of three weeks. Trace amounts of NO3 −/NO2 −were produced and six metabolites were characterized by high performance liquid chromatography (HPLC) using 14C-methylene-imidacloprid and liquid chromatograph-electrospray-mass spectrometer (LC-MS). Two of the metabolites were identified as imidacloprid-guanidine and imidacloprid-urea by HPLC standards and LC-MS. During the experiment, 6-chloronicotinic acid was not produced. Less than 1% of the applied 14C was incorporated into the microbial biomass and no 14CO2 was detected. Strain PC-21, identified as a species of Leifsonia by PCR amplification of a 500 bp sequence of 16s rRNA, cometabolized imidacloprid.

Assessment of the Iowa River's South Fork watershed: Part 1. Water quality

Iowas South Fork watershed is dominated by corn (Zea mays L.) and soybean [Glycine max L. (Merr.)] rotations, and animal feeding operations are common. Artificial subsurface (tile) drainage is extensive; hydric soils cover 54% of the watershed. During spring and early summer, NO3-N concentrations in tile and stream discharge often exceed 20 mg L-1. Total N loads during 2002 to 2005 ranged from 16 to 26 kg NO3-N ha-1 y-1 (14 to 23 lb ac-1 yr-1). Nitrate concentrations increased linearly with log baseflow, effectively a surrogate measure of tile discharge. Phosphorus loads were only 0.4 to 0.7 kg P ha-1 y-1 (0.4 to 0.6 lb ac-1 yr-1), but concentrations commonly exceeded 0.1 mg L-1, a eutrophication-risk threshold. Mean E. coli populations in the stream exceeded 500 cells 100 ml-1 during summer. Statistical comparison of actual nitrate records with independent records generated using regression equations provided modeling efficiencies of 0.91 or less, suggesting performance targets for watershed model validation. Tile drainage is more important in transport of nitrate and dissolved phosphorus than E. coli. Variations in nitrate, phosphorus, and E. coli are uniquely timed, highlighting the complexity of integrated water quality assessments.

Source-Pathway Separation of Multiple Contaminants during a Rainfall-Runoff Event in an Artificially Drained Agricultural Watershed

A watersheds water quality is influenced by contaminant-transport pathways unique to each landscape. Accurate information on contaminant-pathways could provide a basis for mitigation through well-targeted approaches. This study determined dynamics of nitrate-N, total P, Escherichia coli, and sediment during a runoff event in Tipton Creek, Iowa. The watershed, under crop and livestock production, has extensive tile drainage discharging through an alluvial valley. A September 2006 storm yielded 5.9 mm of discharge during the ensuing 7 d, which was monitored at the outlet (19,850 ha), two tile-drainage outfalls (total 1856 ha), and a runoff flume (11 ha) within the sloped valley. Hydrograph separations indicated 13% of tile discharge was from surface intakes. Tile and outlet nitrate-N loads were similar, verifying subsurface tiles dominate nitrate delivery. On a unit-area basis, tile total P and E. coli loads, respectively, were about half and 30% of the outlets; their rapid, synchronous timing showed surface intakes are an important pathway for both contaminants. Flume results indicated field runoff was a significant source of total P and E. coli loads, but not the dominant one. At the outlet, sediment, P, and E. coli were reasonably synchronous. Radionuclide activities of (7)Be and (210)Pb in suspended sediments showed sheet-and-rill erosion sourced only 22% of sediment contributions; therefore, channel sources dominated and were an important source of P and E. coli. The contaminants followed unique pathways, necessitating separate mitigation strategies. To comprehensively address water quality, erosion-control and nitrogen-management practices currently encouraged could be complemented by buffering surface intakes and stabilizing stream banks.

Assessment of the Iowa River's South Fork watershed: Part 2. Conservation practices

Documenting the types and extent of conservation practices in a watershed is necessary to determine their water quality impacts. A conservation practice inventory for the South Fork of the Iowa River, 85% in corn (Zea mays L.) and soybean [Glycine max L. (Merr.)] rotations, showed only 7% of cropland was managed using no-tillage. About 30% of cropland receives manure annually, prior to corn. Surface residue following soybean was usually inadequate (<30%), indicating a key management challenge. About 90% of fields with >34% highly erodible land, subject to USDA conservation compliance, indeed had erosion-control practices installed. Grassed waterways and riparian buffers were common edge-of-field practices, and highly erodible land fields near streams often had multiple practices and rotations including third crops. Yet, while most conservation practices are aimed at controlling runoff, tile drainage is the dominant hydrologic pathway. Resource management systems that address tile drainage as the primary route of nutrient loss need to be developed and encouraged. Better targeting of this pathway could include practices such as nutrient removal wetlands.

Transport and Persistence of Tylosin-Resistant Enterococci, erm Genes, and Tylosin in Soil and Drainage Water from Fields Receiving Swine Manure

Land application of manure from tylosin-treated swine introduces tylosin, tylosin-resistant enterococci, and erythromycin resistant rRNA methylase () genes, which confer resistance to tylosin. This study documents the persistence and transport of tylosin-resistant enterococci, genes, and tylosin in tile-drained chisel plow and no-till agricultural fields treated with liquid swine manure in alternating years. Between 70 and 100% of the enterococci in manure were resistant to tylosin and B concentrations exceeded 10 copies g manure, while the mean F concentrations exceeded 10 copies g manure (T was not detected). The mean concentration of tylosin was 73 ng g manure. Soil collected from the manure injection band closely following application contained >10 copies g soil of both B and F in 2010 and >10 copies g soil after the 2011 application compared to 3 × 10 to 3 × 10 copies g soil in the no-manure control plots. Gene abundances declined over the subsequent 2-yr period to levels similar to those in the no-manure controls. Concentrations of enterococci in tile water were low, while tylosin-resistant enterococci were rarely detected. In approximately 75% of tile water samples, B was detected, and F was detected in 30% of tile water samples, but levels of these genes were not elevated due to manure application, and no difference was found between tillage practices. These results show that tylosin usage increased the short-term occurrence of tylosin-resistant enterococci, genes, and tylosin in soils but had minimal effect on tile drainage water quality in years of average to below average precipitation.

Sorption and Photodegradation Processes Govern Distribution and Fate of Sulfamethazine in Freshwater−Sediment Microcosms

The antibiotic sulfamethazine can be transported from manured fields to surface water bodies. We investigated the degradation and fate of sulfamethazine in pond water using (14)C-phenyl-sulfamethazine in small pond water microcosms containing intact sediment and pond water. We found a 2.7-day half-life in pond water and 4.2-day half-life when sulfamethazine was added to the water (5 mg L(-1) initial concentration) with swine manure diluted to simulate runoff. Sulfamethazine dissipated exponentially from the water column, with the majority of loss occurring via movement into the sediment phase. Extractable sulfamethazine in sediment accounted for 1.9-6.1% of the applied antibiotic within 14 days and then declined thereafter. Sulfamethazine was transformed mainly into nonextractable sediment-bound residue (40-60% of applied radioactivity) and smaller amounts of photoproducts. Biodegradation, as indicated by metabolite formation and (14)CO2 evolution, was less significant than photodegradation. Two photoproducts accounted for 15-30% of radioactivity in the water column at the end of the 63-day study; the photoproducts were the major degradates in the aqueous and sediment phases. Other unidentified metabolites individually accounted for <7% of radioactivity in the water or sediment. Less than 3% of applied radioactivity was mineralized to (14)CO2. Manure input significantly increased sorption and binding of sulfamethazine residues to the sediment. These results show concurrent processes of photodegradation and sorption to sediment control aqueous concentrations and establish that sediment is a sink for sulfamethazine and sulfamethazine-related residues. Accumulation of the photoproducts and sulfamethazine in sediment may have important implications for benthic organisms.

Herbicide and rotation effects on soil and rhizosphere microorganisms and crop yields

Abstract The effects of long-term use of the herbicides, trifluralin and alachlor, on crop yields and microbial populations were determined for continuous and rotated corn and soybeans under field conditions. Seven previous annual herbicide applications produced no lasting differences in populations of soil fungi, cellulolytic bacteria, pectinolytic bacteria, fluorescent Pseudomonas spp., or Gram-positive bacteria. Both herbicides caused temporary fluctuations in the populations of soil and rhizosphere bacteria. The effects of crop rotation on these microbial groups were confined to sampling times following crop residue incorporation. The average yield of rotated soybeans was 12% greater than that of continuous soybeans.

Temporal variability of organic C and nitrate in a shallow aquifer

The loading of organic substrates into shallow aquifers may follow seasonal cycles, which will impact the transport and fate of agrichemicals. The objective of this research was to measure temporal changes in the groundwater dissolved organic C (DOC) and nitrate concentrations. Groundwater monitoring wells were installed and sediment samples from the aquifer were collected in 1991. Sediment samples were used to evaluate denitrification potentials, while water samples were collected at periodic intervals in 1992 and 1993 from the surface of the aquifer. Water samples were analyzed for nitrate-N and DOC-C. Denitrification was observed in sediment amended with nitrate and incubated under anaerobic conditions at 10°C. Addition of algae lazed biomass increased denitrification, establishing that denitrification was substrate limited. In the aquifer, DOC concentrations followed seasonal patterns. DOC concentrations were highest following spring recharge and then decreased. Peak timing indicates that freezing and thawing were responsible for seasonal DOC patterns. These findings show that seasonally driven physical processes, such as freezing and thawing, influence organic substrate transport from surface to subsurface environments, and that this process should be taken into account when assessing agrichemical detoxification rates in shallow aquifers.

Woodchip Denitrification Bioreactors: Impact of Temperature and Hydraulic Retention Time on Nitrate Removal

Woodchip denitrification bioreactors, a relatively new technology for edge-of-field treatment of subsurface agricultural drainage water, have shown potential for nitrate removal. However, few studies have evaluated the performance of these reactors under varied controlled conditions including initial woodchip age and a range of hydraulic retention times (HRTs) and temperatures similar to the field. This study investigated (i) the release of total organic C (TOC) during reactor start up for fresh and weathered woodchips, (ii) nitrate (NO-N) removal at HRTs ranging from 2 to 24 h, (iii) nitrate removal at influent NO-N concentrations of 10, 30, and 50 mg L, and (iv) NO-N removal at 10, 15, and 20°C. Greater TOC was released during bioreactor operation with fresh woodchips, whereas organic C release was low when the columns were packed with naturally weathered woodchips. Nitrate-N concentration reductions increased from 8 to 55% as HRT increased. Nitrate removal on a mass basis (g NO-N m d) did not follow the same trend, with relatively consistent mass removal measured as HRT increased from 1.7 to 21.2 h. Comparison of mean NO-N load reduction for various influent NO-N concentrations showed lower reduction at an influent concentration of 10 mg L and higher NO-N reductions at influent concentrations of 30 and 50 mg L. Nitrate-N removal showed a stepped increase with temperature. Temperature coefficient () factors calculated from NO-N removal rates ranged from 2.2 to 2.9.