Thomas G. Field

Continuous purification of porcine lipase by rotating annular size-exclusion cnromatography

Crude porcine lipase (triacylglycerol lipase, EC 3.1.1.3) was purified in a single-stage Chromatographic process. The purification was accomplished in a batch, as well as in a continuous system. Two types of sizeexclusion packing materials (Sephadex and Sephacryl) were used. The average x-fold increase in purity, and the average recovered activity in the batch Sephadex and Sephacryl experiments were 13.6 and 89.7%, and 34.2 and 98.8%, respectively. The average x-fold increase in purity and the average activity recovered in the continuous Sephadex and Sephacryl experiments were 27.1 and 82.5% and 16.2 and 89%, respectively. Flow visualization experiments were carried out by tagging the protein to be separated with a fluorescent dye. The results from these experiments are also reported in this article.

Fundamentals of Intellectual Property: Cases & Materials

This is approximately the 24th revision of a conventional 472 pp. casebook. Used twice annually since 1999, this edition will be used in 3-semester-hour courses in 2012-13. Noncommercial reproduction of this file is royalty free, but one of the comprehensive statutory supplement is recommended. Also, an inexpensive paperback may be purchased from Amazon, with bulk discounts available through the author.The book flags common themes and critical distinctions among IP components. The goal is to present the most important and durable aspects of the law for the benefit of potential legal specialists as well as generalists. Pursuit of such objectives is informed by over forty years of study and teaching students at every level of technical and legal sophistication. That current policy debates often center on issues raised in the 1800s and earlier has also been helpful. Indeed, much that is truly fundamental remains remarkably constant. Domain names and rights of publicity also receive modest attention. Moreover, from the outset, remedies figure pervasively. Preemption and commercial speech issues are addressed in separate chapters, as well as elsewhere when appropriate.