Thomas R. Unnasch
Harvard University
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Featured researches published by Thomas R. Unnasch.
Molecular and Biochemical Parasitology | 1989
Stefanie E.O. Meredith; Thomas R. Unnasch; Marc Karam; Willy F. Piessens; Dyann F. Wirth
A cloned sequence, pOvs134, was isolated from a genomic library prepared from Onchocerca volvulus of savanna origin in the plasmid pUC9. pOvs134 hybridizes to all the geographic isolates of O. volvulus tested from both the New and the Old World, but not to the species Onchocerca gibsoni, Onchocerca gutturosa, Onchocerca ochengi, Onchocerca cervicalis, the filarial parasites Brugia malayi, or Dirofilaria immitis, nor to human or simuliid DNA. As little as 250 pg of DNA can be detected on a dot blot hybridization, suggesting that pOvs134 is sensitive enough to detect a single third stage larva. DNA sequence analysis of the inserted DNA of pOvs134 revealed that it consisted of twelve examples of a 149-bp repeat. The sequence of this repeat is strikingly similar to that of two O. volvulus genomic clones previously described, one of which has been reported to be specific for forest form O. volvulus, and one of which hybridizes to genomic DNA of several species of Onchocerca. These results suggest that the 149-bp repeat sequence is highly repeated in the genome of O. volvulus, and that variants of this repeat with different specificities exist.
Molecular and Cellular Biology | 1989
J T Joseph; S M Aldritt; Thomas R. Unnasch; O. Puijalon; Dyann F. Wirth
We have identified a conserved, repeated, and highly transcribed DNA element from the avian malarial parasite Plasmodium gallinaceum. The element produced multiple transcripts in both zygotes and asexual blood stages of this parasite. It was found to be highly conserved in all of five malarial species tested and hybridized at reduced stringency to other members of the phylum Apicomplexa, including the genera Babesia, Eimeria, Toxoplasma, and Theileria. The copy number of the element was about 15, and it had a circularly permuted restriction map with a repeat unit length of about 6.2 kilobases. It could be separated from the main genomic DNA by using sucrose gradients and agarose gels, and it migrated separately from the recognized Plasmodium chromosomes on pulse-field gels. In the accompanying paper (S. M. Aldritt, J. T. Joseph, and D. F. Wirth, Mol. Cell. Biol. 9:3614-3620, 1989), evidence is presented that element contains the mitochondrial genes for the protein cytochrome b and a fragment of the large rRNA. We postulate that this element is an episome in the mitochondria of the obligate parasites belonging to the phylum Apicomplexa.
Nucleic Acids Research | 1983
Thomas R. Unnasch; Dyann F. Wirth
Molecular and Biochemical Parasitology | 1986
Jyotsna Shah; Louis Lamontagne; Thomas R. Unnasch; Dyann F. Wirth; Willy F. Piessens
Nucleic Acids Research | 1983
Thomas R. Unnasch; Dyann F. Wirth
Nucleic Acids Research | 1989
Andrew P. Waters; Thomas R. Unnasch; Dyann F. Wirth; Thomas F. McCutchan
Delphion Inc., www.delphion.com | 2001
Sara Lustigman; Eric Pearlman; Thomas R. Unnasch
Molecular and Biochemical Parasitology | 1985
Thomas R. Unnasch; Martha A. McLafferty; Dyann F. Wirth
The Journal of Infectious Diseases | 1986
Bruce M. Greene; Thomas R. Unnasch
Archive | 2013
Benjamin G. Jacob; Robert J. Novak; Laurent Toé; Moussas S. Sanfo; Semiha Caliskan; Rose Tingueria; Alain Pare; Laurent Yameogo; Daniel A. Griffith; Thomas R. Unnasch