Tian-Peng Yin

Diterpenoid alkaloids from Aconitum vilmorinianum

Diterpenoid alkaloids, named vilmorines A-D, in addition to fifteen known alkaloids, were isolated from roots of Aconitum vilmorinianum. Their structures were established on the basis of extensive spectroscopic analyses. Antibacterial and antioxidant studies on isolated compounds were also carried out.

A new C19-diterpenoid alkaloid from the roots of Aconitum duclouxii

A new C19-diterpenoid alkaloid, ducloudine F (1), was obtained from the roots of Aconitum duclouxii, along with eight known alkaloids (2–9) isolated from this species for the first time. Their structures were established on the basis of extensive spectroscopic analyses. The antimicrobial activities of these compounds were investigated.

Three new diterpenoid alkaloids from the roots of Aconitum duclouxii.

Three new C19-diterpenoid alkaloids, ducloudines C (1), D (2), and E (3), were isolated from the roots of Aconitum duclouxii. Their structures were established on the basis of extensive spectroscopic analyses. Ducloudine C (1) is the first aconitine-type C19-diterpenoid alkaloid with a C = O group at C-3 and a C = C bond between C-1 and C-2. All compounds were tested for their biological activities against one pathogenic fungi and two pathogenic bacteria.

A new flavone C-glycoside and a new bibenzyl from Bulbophyllum retusiusculum.

Abstract A new flavone C-glycoside, apigenin 6-C-α-arabinofuranosyl 8-C-α-arabinopyranoside (1) and a new bibenzyl, bulbotetusine (2), were isolated from the tubers of Bulbophyllum retusiusculum. Their structures were established on the basis of extensive spectroscopic analyses. The absolute configuration of 2 was determined by the comparison of experimental and calculated electronic circular dichroism. Compounds 1 and 2 showed no obvious cytotoxic activity against any five human tumour cell lines with IC50 values >40 μM.

Two new glucosides from the pellicle of the walnut (Juglans regia)

A new α-tetralonyl glucoside, 6′-O-acetyl-juglanoside E (1), and a new dihydrophaseic acid glucoside, dihydrophaseic acid 1-O-(6-O-acetyl)-glucopyranoside (2), together with two known ones, juglanoside E (3) and dihydrophaseic acid (4), were isolated from the pellicle of the walnut (Juglans regia). The structures of the new compounds were elucidated by comprehensive spectroscopic analysis, including IR, HRESIMS, 1D and 2D NMR data.

A new C20-diterpenoid alkaloid from the lateral roots of Aconitum carmichaeli

Abstract A new C20-diterpenoid alkaloid carmichaedine (1) and six known alkaloids (2–7) were isolated from the lateral roots of Aconitum carmichaeli. Their structures were established on the basis of extensive spectroscopic analyses. Compound 1 exhibited potent antibacterial activity against Bacillus subtilis with minimum inhibitory concentration of 8 μg/mL.

Improving the antioxidant activity and enriching salvianolic acids by the fermentation of Salvia miltiorrhizae with Geomyces luteus

The antioxidant activities and total phenolic content of fermented Salvia miltiorrhiza with fungus Geomyces luteus were investigated. The results revealed that G. luteus fermentation could significantly improve the antioxidant activity and total phenolic content of S. miltiorrhiza. The main antioxidant constituents were characterized by spectroscopic analysis as salvianolic acids. High-performance liquid chromatography (HPLC) quantification also showed the enhanced content of salvianolic acid B after fermentation. The present study suggests that G. luteus fermentations are effective in the S. miltiorrhiza salvianolic acids’ enrichment process.中文概要目的研究真菌固态发酵对丹参抗氧化活性的影响及探 索丹酚酸富集的途径。创新点该研究发现真菌G. luteus 固态发酵可增加丹参的 抗氧化活性及有效地富集丹酚酸B,为丹参更有 效的开发利用提供了新思路。方法采用20 种真菌对丹参进行固态发酵,以两种抗 氧化模型(二苯代苦味酰基自由基(DPPH•)和 2,2′-联氮基双(3-乙基苯并噻唑啉-6-磺酸)二铵 盐自由基(ABTS+•))测定发酵物的抗氧化活性; 采用Folin-Ciocaltea 比色法测定发酵物的总酚含 量;以多种波谱学方法(紫外光谱(UV)、红外 光谱(IR)和核磁共振波谱(NMR))和高效液 相色谱法(HPLC)表征发酵前后丹参化学成分 的变化。结论抗氧化活性测试和总酚测定结果表明, G. luteus 发酵可有效地增加丹参的抗氧化活性和总酚含 量。UV、IR 及NMR 等波谱表征发现,在丹参经 G. luteus 发酵后,丹酚酸类化合物的含量提高了; 同时HPLC 测定也发现,丹酚酸B含量显著增加。 这些结果表明, G. luteus 发酵可实现丹酚酸B 的 富集。

Alkaloids with antioxidant activities from Aconitum handelianum

Abstract A new C20-diterpenoid alkaloid handelidine (1) and twenty-seven known alkaloids (2–28) were isolated from the roots of Aconitum handelianum. Their structures were established on the basis of extensive spectroscopic analyses. The study indicated that denudatine-type C20-diterpenoid alkaloids with vicinal-triol system and benzyltetrahydroisoquinoline alkaloids exhibited significant antioxidant activities measured by three antioxidant test systems. The aconitine-type C19-diterpenoid alkaloids could serve as potential secondary antioxidants for their strong binding effects to metal ions.

Five New Phenolic Compounds with Antioxidant Activities from the Medicinal Insect Blaps rynchopetera

Five new phenolic compounds rynchopeterines A–E (1–5), in addition to thirteen known phenolics, were isolated from Blaps rynchopetera Fairmaire, a kind of medicinal insect utilized by the Yi Nationality in Yunnan Province of China. Their structures were established on the basis of extensive spectroscopic analyses (1D and 2D NMR, HR-MS, IR) along with calculated electronic circular dichroism method. Rynchopeterines A–E (1–4) exhibited significant antioxidant activities with IC50 values of 7.67–12.3 μg/mL measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Besides, rynchopeterines B (2) and C (3) showed mild cytotoxicity against tumor cell Caco-2 and A549.

A new polyketide glycoside from the rhizospheric Clonostachys rogersoniana associated with Panax notoginseng

Abstract A new polyketide glycoside rogerson A (1), along with two known compounds, rogerson B (2) and (22E)-5α,8α-epidioxyergosta-6,22-dien-3β-ol (3), was obtained from the solid fermentation of Clonostachys rogersoniana, which was isolated from the rhizosphere soil of Panax notoginseng. Rogerson B (2) was isolated for the first time from a natural source. Their structure was determined by extensive analyses of NMR and MS studies. Compounds 1 and 2 were tested for its cytotoxicity against five human cancer cell lines.