Tianyuan Zhang
Sun Yat-sen University
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Tianyuan Zhang.
Gene | 2012
Suishan Yang; Zhenwang Liu; X. P. Yi; Ailian Zhang; Tianyuan Zhang; Jinxian Luo; Zehua Zhang; Jincheng Shen; Huixiang Yin; Liping Chen
The present study reports the cloning and sequencing of lac2 from Bacillus subtilis. The gene is composed of 1542 bp and encodes a 514-amino acid protein. The gene has 86% homology with a published laccase with GeneID 936023. The lac2 gene was deposited in GenBank as a new nucleotide sequence. This new sequence was cloned into the multiple cloning site of pPIC9K to generate pPIC9K-lac2, which was then transformed into Pichia pastoris GS115 via electroporation. The recombinant GS115 (pPIC9K-lac2) was grown initially in BMGY medium and transferred to BMMY to induce gene expression for 48 h. The recombinant Lac2 protein shows laccase activity with α-naphthol and guaiacol as substrates. The optimal pH is between 3.2 and 4.7, and the optimal temperature is 25°C for enzyme reaction.
Gene | 2012
Huixiang Yin; Zhenwang Liu; Ailian Zhang; Tianyuan Zhang; Jinxian Luo; Jincheng Shen; Liping Chen; Bing Zhou; Xian Fu; Ce-Yi Fu; Zehua Zhang
Canstatin-N DNA fragment amplified from human genome was inserted into the MCS of pGAP9K*, an intracellular expression vector of Pichia pastoris, to generate pGAP9K*-can-N which was then transformed into P. pastoris GS115 by electroporation. A transformant was chosen as an engineering strain from the plate containing G418 (700 μg/ml). D-sorbitol was selected as the only carbon source. The fermentation was carried out in a 50 L bioreactor at a 20 L working volume. After 48 h fermentation with continuous feeding of 25% (w/v) D-sorbitol and 0.8% PTM4, the cell grew to A(600)=178 and intracellularly expressed Canstatin-N reached 780 mg/L. Snail enzyme was combined with water to crack P. pastoris and to release intracellular proteins. The purified recombinant Canstatin-N inhibited CAM angiogenesis and induced significant apoptosis of the human umbilical vein endothelial cell (EVC340).
Molecular Biology Reports | 2009
Ailian Zhang; Jinxian Luo; Tianyuan Zhang; Ying-Wen Pan; Yanhua Tan; Ce-Yi Fu; Fazhi Tu
Molecular Biology Reports | 2012
Zhenwang Liu; Huixiang Yin; X. P. Yi; Ailian Zhang; Jinxian Luo; Tianyuan Zhang; Ce-Yi Fu; Zehua Zhang; Jincheng Shen; Liping Chen
Molecular Biology Reports | 2009
Ailian Zhang; Tianyuan Zhang; Jinxian Luo; Ce-Yi Fu; Zhi Qu; Guohui Yi; Dong-Xiao Su; Fazhi Tu; Ying-Wen Pan
Molecular Biology Reports | 2011
D. X. Su; Ailian Zhang; G. H. Yi; Zhenwang Liu; Jinxian Luo; L. Y. Rao; Tianyuan Zhang; Z. J. Zhou
Archive | 2010
Ailian Zhang; Fazhi Tu; Tianyuan Zhang; Guohui Yi; Jinxian Luo; Zhi Qu
Archive | 2010
Zhenwang Liu; Jinxian Luo; Yanhua Tan; X. P. Yi; Ailian Zhang; Tianyuan Zhang; Zehua Zhang
Archive | 2010
Ailian Zhang; Guohui Yi; Jinxian Luo; Tianyuan Zhang; Huixiang Yin
Archive | 2012
Ailian Zhang; Zehua Zhang; Jincheng Shen; Tianyuan Zhang; Jinxian Luo