Tim A. Smitka
Eli Lilly and Company
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Featured researches published by Tim A. Smitka.
Tetrahedron | 1996
Rosanne Bonjouklian; Tim A. Smitka; Ann H. Hunt; John L. Occolowitz; Thomas J. Perun; Lawrence E. Doolin; Stephanie Stevenson; Lisa Knauss; Ranmali Wijayaratne; Stanley Szewczyk; Gregory M. L. Patterson
Abstract The isolation and structure elucidation of A90720A, 1 , a cyclic depsipeptide from the terrestrial blue-green alga Microchaete loktakensis is described. 1 was isolated on the basis of its thrombin inhibitory activity and its structure was determined using spectral methods and amino acid analysis. The absolute configurations of the constituent amino acids were found to be as follows: the nucleus contains L -threonine, L -arginine, L -3-amino-6-hydroxy-2-piperidone (Ahp, i.e., glutamic acid-γ-car☐aldehyde), L -leucine, L -N-methyl-tyrosine and L -valine. The side chain off the L -threonine amino group contains D -leucine, followed by a 3-O-sulfated R -glycerate moiety.
Biotechnology Progress | 2013
Erik K. Read; Scott A. Bradley; Tim A. Smitka; Cyrus Agarabi; Scott Lute; Kurt Brorson
Fermentanomics, or a global understanding of a culture state on the molecular level empowered by advanced techniques like NMR, was employed to show that a model hybridoma culture supplied with glutamine and glucose depletes aspartate, cysteine, methionine, tryptophan, and tyrosine during antibody production. Supplementation with these amino acids prevents depletion and improves culture performance. Furthermore, no significant changes were observed in the distribution of glycans attached to the IgG3 in cultures supplemented with specific amino acids, arguing that this strategy can be implemented without fear of impact on important product quality attributes. In summary, a targeted strategy of quantifying media components and designing a supplementation strategy can improve bioprocess cell cultures when enpowered by fermentanomics tools. Published 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:745–753, 2013
Phytochemistry | 1989
Richard E. Moore; Xu-qiang G. Yang; Gregory M. L. Patterson; Rosanne Bonjouklian; Tim A. Smitka
Abstract Dechlorofontonamide, anhydrohapaloxindoles B and M, and hapalonamides G, H, and V have been isolated as minor indole alkaloids from a cultured strain of the terrestrial blue-green alga Hapalosiphon fontinalis .
Journal of the American Chemical Society | 1994
Klemens Stratmann; Richard E. Moore; Rosanne Bonjouklian; Jack B. Deeter; Gregory M. L. Patterson; Stacey Shaffer; Charles D. Smith; Tim A. Smitka
Journal of Organic Chemistry | 1987
Richard E. Moore; Chad Cheuk; Xu Qiang G. Yang; Gregory M. L. Patterson; Rosanne Bonjouklian; Tim A. Smitka; Jon S. Mynderse; Ron S. Foster; Noel D. Jones
Journal of Organic Chemistry | 1992
Tim A. Smitka; Rosanne Bonjouklian; Larry E. Doolin; Noel D. Jones; Jack B. Deeter; Wesley Y. Yoshida; Michèle R. Prinsep; Richard E. Moore; Gregory M. L. Patterson
Journal of the American Chemical Society | 2010
Scott A. Bradley; Anli Ouyang; Jennifer Purdie; Tim A. Smitka; Tongtong Wang; Andreas Kaerner
The Journal of Antibiotics | 1988
Tim A. Smitka; Jack B. Deeter; Ann H. Hunt; Frederick P. Mertz; Robert M. Ellis; Laverne Dwaine Boeck; Raymond C. Yao
Chemistry & Biology | 1994
Angela Y. Lee; Tim A. Smitka; Rosanne Bonjouklian; Jon Clardy
The Journal of Antibiotics | 1992
Tim A. Smitka; Rosanne Bonjouklian; Thomas J. Perun; Ann H. Hunt; Ronald S. Foster; Jon S. Mynderse; Raymond C. Yao