Ting-Kuo Lee

Quantitative 3D imaging of whole, unstained cells by using X-ray diffraction microscopy

Microscopy has greatly advanced our understanding of biology. Although significant progress has recently been made in optical microscopy to break the diffraction-limit barrier, reliance of such techniques on fluorescent labeling technologies prohibits quantitative 3D imaging of the entire contents of cells. Cryoelectron microscopy can image pleomorphic structures at a resolution of 3–5 nm, but is only applicable to thin or sectioned specimens. Here, we report quantitative 3D imaging of a whole, unstained cell at a resolution of 50–60 nm by X-ray diffraction microscopy. We identified the 3D morphology and structure of cellular organelles including cell wall, vacuole, endoplasmic reticulum, mitochondria, granules, nucleus, and nucleolus inside a yeast spore cell. Furthermore, we observed a 3D structure protruding from the reconstructed yeast spore, suggesting the spore germination process. Using cryogenic technologies, a 3D resolution of 5–10 nm should be achievable by X-ray diffraction microscopy. This work hence paves a way for quantitative 3D imaging of a wide range of biological specimens at nanometer-scale resolutions that are too thick for electron microscopy.

Scale-invariant quantum anomalous Hall effect in magnetic topological insulators beyond the two-dimensional limit.

We investigate the quantum anomalous Hall effect (QAHE) and related chiral transport in the millimeter-size (Cr(0.12)Bi(0.26)Sb(0.62))₂Te₃ films. With high sample quality and robust magnetism at low temperatures, the quantized Hall conductance of e²/h is found to persist even when the film thickness is beyond the two-dimensional (2D) hybridization limit. Meanwhile, the Chern insulator-featured chiral edge conduction is manifested by the nonlocal transport measurements. In contrast to the 2D hybridized thin film, an additional weakly field-dependent longitudinal resistance is observed in the ten-quintuple-layer film, suggesting the influence of the film thickness on the dissipative edge channel in the QAHE regime. The extension of the QAHE into the three-dimensional thickness region addresses the universality of this quantum transport phenomenon and motivates the exploration of new QAHE phases with tunable Chern numbers. In addition, the observation of scale-invariant dissipationless chiral propagation on a macroscopic scale makes a major stride towards ideal low-power interconnect applications.

A guided Monte Carlo approach to optimization problems

Conversational agent is a system that provides user with proper information and maintains the context of dialogue based on natural language. When experts design the network for conversational agent of a domain, the network is usually very complicated and is hard to be understood. So the simplification of network by separating variables in the domain is helpful to design the conversational agent more efficiently. Composing Bayesian network as two stages, we aim to design conversational agent easily and analyze user’s query in detail. Also, by using previous information of dialogue, it is possible to maintain the context of conversation. Actually implementing it for a guide of web pages, we can confirm the usefulness of the proposed architecture for conversational agent.

Enhanced thermoelectric power in dual-gated bilayer graphene.

The thermoelectric power of a material, typically governed by its band structure and carrier density, can be varied by chemical doping that is often restricted by solubility of the dopant. Materials showing large thermoelectric power are useful for many industrial applications, such as the heat-to-electricity conversion and the thermoelectric cooling device. Here we show a full electric-field tuning of thermoelectric power in a dual-gated bilayer graphene device resulting from the opening of a band gap by applying a perpendicular electric field on bilayer graphene. We uncover a large enhancement in thermoelectric power at a low temperature, which may open up a new possibility in low temperature thermoelectric application using graphene-based device.

Perinatal mortality and prevalence of major congenital malformations of twins in Taipei City

In order to examine perinatal mortality and prevalence of major congenital malformations in twins, deliveries in four teaching hospitals in Taipei City were studied. Among a total of 73,264 deliveries from October 1985 to June 1989, there were 844 pairs of twins. The zygosity of the twin pairs was determined by sex, placentation and 12 red blood cell antigens. There were 482 MZ and 252 DZ twin pairs identified, but the zygosity of a further 110 twin pairs was indeterminable due to lack of information on plancentation and/or blood types. A total of 4,573 singletons delivered in one study hospital from July 1986 to June 1987 were also studied as controls. The perinatal mortality rate was 7.5% for MZ twins, 1.4% for DZ twins, and 0.7% for singletons. The concordance rate of perinatal death was significantly higher in MZ (60%) than in DZ (0%) twins. The prevalence of major congenital malformations was 2.7% for MZ twins, 1.0% for DZ twins, and 0.6% for singletons. The concordance rate of major congenital malformations was 18% for MZ twins, but no DZ pair was concordant in any major congenital malformation. The concordance rate of facial clefts was 29% for MZ twins. There were 2 sets of conjoined twins giving a prevalence rate of 2.7 per 100,000 deliveries. These findings showing the prevalence of perinatal mortality and major congenital malformation to be highest in MZ twins, intermediate in DZ twins and lowest in singletons, suggest the importance of intrauterine environments in the determination of perinatal mortality and congenital malformations.

Guided simulated annealing method for optimization problems

Incorporating the concept of order parameter of the mean-field theory into the simulated annealing method, we present an optimization algorithm, the guided simulated annealing method. In this method mean-field order parameters are calculated to guide the configuration search for the global minimum. Allowing fluctuations and improvement of mean-field values iteratively, this method successfully identifies global minima for several difficult optimization problems. Application of this method to the HP lattice-protein model has found another lowest-energy state for an N=100 sequence that was not found by other methods before. Results for spin glass models are also presented which show improvement over the previous results.

Thermopower of gapped bilayer graphene

We calculate thermopower of clean and impure bilayer graphene systems. Opening a band gap through the application of an external electric field is shown to greatly enhance the thermopower of bilayer graphene, which is more than four times that of the monolayer graphene and gapless bilayer graphene at room temperature. The effect of scattering by dilute charged impurities is discussed in terms of the self-consistent Born approximation. Temperature dependence of the thermopower is also analyzed.

Surface Spectral Function in the Superconducting State of a Topological Insulator

We discuss the surface spectral function of superconductors realized from a topological insulator, such as the copper-intercalated Bi

Theory for Slightly Doped Antiferromagnetic Mott Insulators

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Disappearance of nodal gap across the insulator-superconductor transition in a copper-oxide superconductor

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