Tobias Pöhlmann

Comparative Effects of l-Tryptophan and 1-Methyl-Tryptophan on Immunoregulation Induced by Sperm, Human Pre-implantation Embryo and Trophoblast Supernatants

Problem: The hypothesis that indoleamine 2,3‐dioxygenase (IDO) is necessary to regulate lymphocyte functions at the feto‐maternal interface has been postulated, although a possible role of tryptophan (Trp) depletion in the T‐cell tolerance during insemination as well as implantation has not been previously investigated.

Short interfering-RNA versus short toxic-RNA

ZusammenfassungZellspezifität und delivery von siRNA sind noch immer bestehende Herausforderungen für deren therapeutische Anwendung. Die neue Technologie einer zielzellspezifischen siRNA erlaubt es, siRNA gezielt zum Abtöten von z. B. Tumorzellen zu verwenden.AbstractCell specificity and delivery of siRNA are still major challenges for therapeutic applications of siRNA. The new cell specific siRNA technology can be used to induce cell death, for example in tumor cells only.

A refined method for lymphocyte isolation from the placenta

OBJECTIVES:  Analysis of placental lymphocytesfrom different compartments is crucial for many questions in reproductive immunology. A separation of lymphocytes after tissue disaggregations leads to a mixture of maternal and fetal lymphocytes, which are difficult to distinguish by simple methods. HLA‐A2 is expressed by 29% (Romanians) – 45% (Spanish) of a Caucasian population and might be a marker for this approach.