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Featured researches published by Todd A. Taylor.


Journal of Chromatography B: Biomedical Sciences and Applications | 1997

Immunoassay for thyroxine (T4) in serum using capillary electrophoresis and micromachined devices

Dieter Schmalzing; Lance Koutny; Todd A. Taylor; Wassim Nashabeh; Martin Fuchs

As part of our ongoing effort to develop electrophoretic assay technology for clinical diagnostics, we describe a competitive immunoassay for the determination of serum thyroxine (T4) based on electrophoresis and laser induced fluorescence (LIF). Measurements of total T4 are useful for the clinical evaluation of thyroid function. A fluorescein thyroxine conjugate was utilized in conjunction with a polyclonal antibody preparation as assay reagents. Capillary electrophoresis (CE) conditions tolerant of the direct injection of serum without extraction or other sample preparation steps were developed and used for quantitation of total T4 in serum. We have been exploring the use of micromachined devices with arrays of channels for high assay throughput. Our assay protocol was carried in a microchip format. The results illustrate that gains in speed can be additionally achieved, with the electrophoretic separation of free from bound labelled T4 being performed in about 15 s for serum samples.


Applied Spectroscopy | 1994

SPECTROSCOPIC PROPERTIES OF WO3 THIN FILMS : POLARIZED FT-IR/ATR, X-RAY DIFFRACTION, AND ELECTRONIC ABSORPTION

Todd A. Taylor; Howard H. Patterson

The Fourier transform infrared/micro-attenuated total reflectance (FT-IR/mATR), X-ray diffraction (XRD), and electronic absorption properties of thin tungsten oxide films are characterized. Thin films of tungsten oxide (100–500 Å) deposited on SiO2 exhibit a different orientation or structure than thicker films. A p-polarized longitudinal optical (LO) mode at 970 cm−1 occurs in all ATR spectra of WO3 thin films and is one of the strongest IR bands in the spectra. The spectroscopic properties of tungsten oxide films are characterized as a function of substrate and heat treatment.


Science of The Total Environment | 1992

Comparison of soil fulvic acids using synchronous scan fluorescence spectroscopy, FTIR, titration and metal complexation kinetics

Howard H. Patterson; Christopher S. Cronan; Sukla Lakshman; B.J. Plankey; Todd A. Taylor

Abstract Fulvic acids isolated from four contrasting forest soil environments (northern coniferous, northern hardwoods, southern coniferous, and southern hardwoods) have been examined using synchronous scan fluorescence spectroscopy (SSFS), FTIR, potentiometric titration, and metal complexation kinetics as a function of metal ion concentration and pH. SSFS methods yielded two distinct emission peaks for fulvic acids, thus providing a more sensitive probe than ordinary fluorescence for the analysis of soluble humic materials. Based on SSFS analysis, calculated Al 3+ binding constants for the different fulvic acids varied by 60% for the high-energy fluorescence site and by 200% for the low-energy fluorescence site. Kinetic fluorescence experiments indicated less than a 10% difference in the observed rate parameters for Al 3+ binding with the four fulvic acids. Average proton dissociation constants for the four fulvic acids varied by a factor of two, but were consistently larger for the hardwood samples and for samples from the southern sites. Likewise, Al 3+ binding constants were larger for the hardwood and southern sample sets. Infrared ATR (attenuated total reflectance) and SSFS results suggested that metal ions like Al 3+ bind to fulvic acid at multiple sites corresponding to salicylic/phthalic acid type moieties as well as lower energy delocalized sites.


Analytica Chimica Acta | 1993

Determination of aqueous aluminum with the fluorescent chelating ligand, 2-hydroxy-1-carbazole carboxylate

Todd A. Taylor; Howard H. Patterson

Abstract The compound 2-hydroxy-1-carbazole carboxylate (HCC−) exhibits a shift in its fluorescence spectrum upon binding to aluminum in aqueous solution. Fluorescence studies at a variety of solution pHs and aluminum concentrations have been used to detemrine the most probable species in solution and binding constants of the complexes formed. The fluorescent chelating agent described in this paper can be used for the rapid determination of unperturbed free aluminium in aqueous solution.


Analytica Chimica Acta | 1993

Determination of aqueous aluminum with the fluorescent chelating ligand, 2-hydroxy-1-carbazole carboxylate: Part II. Application of ratio fluorescence spectroscopy

Todd A. Taylor; Howard H. Patterson; Christopher S. Cronan; Carl L. Schofield

Abstract A ratio fluorescence (RF) technique is developed for the measurement of low aqueous concentrations of free aluminium ion ([Al(H 2 O) 3+ 6 ]) with little perturbation to the analyte solution. A small amount of the fluorescent aluminum-binding chelating agent 2-hydroxy-1-carbazole carboxylate (HCC − ) is added to an analyte solution and the solution fluorescence is measured at two different wavelength settings. The ratio of these two fluorescence intensities is used in conjunction with the solution pH and ionic strength to determine the aluminum concentration. The lower limit of measuring [Al(H 2 O) 3+ 6 ] by this technique is 0.7 μM at pH 4 and 0.06 μM at pH 5. Effects of potential interferences present in natural water solutions are characterized, and the RF method is compared with a standard chemical extraction method of aluminum speciation in aged aluminum hydroxide solutions.


Analytical Chemistry | 1996

Microchip electrophoretic immunoassay for serum cortisol.

Lance Koutny; Dieter Schmalzing; Todd A. Taylor; Martin Fuchs


Archive | 1997

Capillary electrophoresis enzyme immunoassay

Fred E. Regnier; Xian-Wei Yao; Todd A. Taylor; Martin Fuchs; Dieter Schmalzing; Lance Koutny; Wassim Nashabeh


Archive | 1999

Apparatus and methods for sample analysis

Todd A. Taylor; William W. Carson; Lance Koutny


Archive | 1999

Apparatus and method for sample delivery

Todd A. Taylor; William W. Carson; Xian-Wei Yao


Analytical Chemistry | 1987

Excitation resolved synchronous fluorescence analysis of aromatic compounds and fuel oil

Todd A. Taylor; Howard H. Patterson

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Lance Koutny

Massachusetts Institute of Technology

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Dieter Schmalzing

Massachusetts Institute of Technology

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