Tom Clifford

The potential benefits of red beetroot supplementation in health and disease

In recent years there has been a growing interest in the biological activity of red beetroot (Beta vulgaris rubra) and its potential utility as a health promoting and disease preventing functional food. As a source of nitrate, beetroot ingestion provides a natural means of increasing in vivo nitric oxide (NO) availability and has emerged as a potential strategy to prevent and manage pathologies associated with diminished NO bioavailability, notably hypertension and endothelial function. Beetroot is also being considered as a promising therapeutic treatment in a range of clinical pathologies associated with oxidative stress and inflammation. Its constituents, most notably the betalain pigments, display potent antioxidant, anti-inflammatory and chemo-preventive activity in vitro and in vivo. The purpose of this review is to discuss beetroot’s biological activity and to evaluate evidence from studies that specifically investigated the effect of beetroot supplementation on inflammation, oxidative stress, cognition and endothelial function.

Effects of Montmorency tart cherry (Prunus Cerasus L.) consumption on vascular function in men with early hypertension

BACKGROUND Tart cherries contain numerous polyphenolic compounds that could potentially improve endothelial function and reduce cardiovascular disease risk. OBJECTIVE We sought to examine the acute effects of Montmorency tart cherry (MC) juice on vascular function in subjects with early hypertension. DESIGN A placebo-controlled, blinded, crossover, randomized Latin square design study with a washout period of ≥14 d was conducted. Fifteen men with early hypertension [systolic blood pressure (SBP) ≥130 mm Hg, diastolic blood pressure ≥80 mm Hg, or both] received either a 60-mL dose of MC concentrate or placebo. Microvascular reactivity (laser Doppler imaging with iontophoresis), arterial stiffness (pulse wave velocity and analysis), blood pressure, and phenolic acid absorption were assessed at baseline and at 1, 2, 3, 5, and 8 h postconsumption. RESULTS MC consumption significantly lowered SBP (P < 0.05) over a period of 3 h, with peak reductions of mean ± SEM 7 ± 3 mm Hg 2 h after MC consumption relative to the placebo. Improvements in cardiovascular disease risk factors were closely linked to increases in circulating protocatechuic and vanillic acid at 1-2 h. CONCLUSIONS MC intake acutely reduces SBP in men with early hypertension. These benefits may be mechanistically linked to the actions of circulating phenolic acids. This study provides information on a new application of MCs in health maintenance, particularly in positively modulating SBP. This trial was registered at clinicaltrials.gov as NCT02234648.

Effects of Beetroot Juice on Recovery of Muscle Function and Performance between Bouts of Repeated Sprint Exercise

This study examined the effects of beetroot juice (BTJ) on recovery between two repeated-sprint tests. In an independent groups design, 20 male, team-sports players were randomized to receive either BTJ or a placebo (PLA) (2 × 250 mL) for 3 days after an initial repeated sprint test (20 × 30 m; RST1) and after a second repeated sprint test (RST2), performed 72 h later. Maximal isometric voluntary contractions (MIVC), countermovement jumps (CMJ), reactive strength index (RI), pressure-pain threshold (PPT), creatine kinase (CK), C-reactive protein (hs-CRP), protein carbonyls (PC), lipid hydroperoxides (LOOH) and the ascorbyl free radical (A•−) were measured before, after, and at set times between RST1 and RST2. CMJ and RI recovered quicker in BTJ compared to PLA after RST1: at 72 h post, CMJ and RI were 7.6% and 13.8% higher in BTJ vs. PLA, respectively (p < 0.05). PPT was 10.4% higher in BTJ compared to PLA 24 h post RST2 (p = 0.012) but similar at other time points. No group differences were detected for mean and fastest sprint time or fatigue index. MIVC, or the biochemical markers measured (p > 0.05). BTJ reduced the decrement in CMJ and RI following and RST but had no effect on sprint performance or oxidative stress.

Antioxidant-rich beetroot juice does not adversely affect acute neuromuscular adaptation following eccentric exercise

ABSTRACT This study examined the effects of beetroot juice on the repeated bout effect (RBE) to eccentric exercise. Twenty-nine recreationally active males performed two bouts of 100-drop jumps, separated by 14–21 days. Using a double-blind, independent groups design, participants consumed either a higher dose beetroot juice (H-BT; 250 ml, n = 10), a lower dose beetroot juice (L-BT; 125 ml, n = 9) or an isocaloric placebo (PLA; 250 ml, n = 10) for 3 days after bout 1; no drinks were consumed after bout 2. Maximal isometric voluntary contraction (MIVC), countermovement jump (CMJ), pressure-pain threshold (PPT) and creatine kinase (CK) were measured pre, post, 24, 48 and 72 h following both bouts. In bout 2, CMJ and MIVC recovered quicker and CK activity was attenuated (versus bout 1) (P < 0.05) in all groups, demonstrating an RBE. At 24 h post bout 1, MIVC was 84.1 ± 16.1, 83.6 ± 11.6, 79.7 ± 15.1% relative to baseline values in the H-BT, L-BT and PLA groups, respectively; at 24 h post bout 2, MIVC recovered to 90.7 ± 13.7, 92.9 ± 6.9, 87.8 ± 6.9, in the H-BT, L-BT and PLA groups, respectively. These findings suggest that supplementation with antioxidant-rich beetroot juice does not adversely affect acute adaptations to a bout of eccentric exercise.

Minimal muscle damage after a marathon and no influence of beetroot juice on inflammation and recovery

This study examined whether beetroot juice (BTJ) would attenuate inflammation and muscle damage following a marathon. Using a double blind, independent group design, 34 runners (each having completed ca. ∼16 previous marathons) consumed either BTJ or an isocaloric placebo (PLA) for 3 days following a marathon. Maximal isometric voluntary contractions (MIVC), countermovement jumps (CMJ), muscle soreness, serum cytokines, leucocytosis, creatine kinase (CK), high sensitivity C-reactive protein (hs-CRP), and aspartate aminotransferase (AST) were measured pre, post, and 2 days after the marathon. CMJ and MIVC were reduced after the marathon (P < 0.05), but no group differences were observed (P > 0.05). Muscle soreness was increased in the day after the marathon (BTJ; 45 ± 48 vs. PLA; 46 ± 39 mm) and had returned to baseline by day 2, irrespective of supplementation (P = 0.694). Cytokines (interleukin-6; IL-6, interleukin-8, tumour necrosis factor-α) were increased immediately post-marathon but apart from IL-6 had returned to baseline values by day 1 post. No interaction effects were evident for IL-6 (P = 0.213). Leucocytes increased 1.7-fold after the race and remained elevated 2 days post, irrespective of supplement (P < 0.0001). CK peaked at 1 day post marathon (BTJ: 965 ± 967, and PLA: 1141 ± 979 IU·L-1) and like AST and hs-CRP, was still elevated 2 days after the marathon (P < 0.05); however, no group differences were present for these variables. Beetroot juice did not attenuate inflammation or reduce muscle damage following a marathon, possibly because most of these indices were not markedly different from baseline values in the days after the marathon.

The Effects of 120 Minutes of Simulated Match Play on Indices of Acid-Base Balance in Professional Academy Soccer Players

Abstract Harper, LD, Clifford, T, Briggs, MA, McNamee, G, West, DJ, Stevenson, E, and Russell, M. The effects of 120 minutes of simulated match play on indices of acid-base balance in professional academy soccer players. J Strength Cond Res 30(6): 1517–1524, 2016—This study investigated the changes in indices of acid-base balance during 120 minutes of simulated soccer match play that included a 30 minute extra-time (ET) period. Eight English Premier League academy soccer players participated in a simulated soccer match that required varying intensities of intermittent exercise including 15-m sprints and soccer dribbling throughout. Blood samples were obtained before (i.e., baseline and pre-exercise) and throughout exercise (i.e., 15, 30, 45, 60, 75, 90, 105, and 120 minutes), and at half time. Sprint speeds over 15 m reduced in ET compared to the first half (−0.39 ± 0.37 m·s−1, −7 ± 6%, p = 0.021) but not the second half (−0.18 ± 0.25 m·s−1, −3 ± 4%, p = 0.086). At 105 minutes, blood lactate concentrations reduced compared with that in the opening 30 minutes (−0.9 to −1.2 mmol·L−1, p ⩽ 0.05). Blood pH (−0.03 to −0.04 units), base excess (−0.95 to −1.48 mmol·L−1), and bicarbonate concentrations (−0.9 ± 0.8 mmol·L−1) were depressed at 120 minutes compared with those at 105 minutes, baseline and half time (all p ⩽ 0.05). There were no significant correlations between changes in acid-base balance and sprint speed (all p > 0.05). Although the perturbations in acid-base balance during ET were statistically significant, the decreases in blood pH, lactate, base excess, and bicarbonate concentrations may not represent metabolic acidosis or impairments in buffering capacity that are likely to explain reduced physical performance. Further research is warranted to investigate mechanisms of fatigue during ET and to develop interventions that attenuate decrements in performance.

The independent effects of match location, match result and the quality of opposition on subjective wellbeing in under 23 soccer players: a case study

ABSTRACT This study examined if subjective wellbeing in soccer players was affected by match location, match result and opposition quality before a match (PRE), 1 day after (POST-1), and 3 days after a match (POST-3). Eleven professional male soccer players from the under 23 squad playing in the Premier League 2 division completed a wellbeing questionnaire before and after 17 matches. Match training load (session-rating perceived exertion) was not different, regardless of the location, result, or quality of opposition faced (P > 0.05). Subjective wellbeing was not different at PRE (P > 0.05); however, at POST-1 and POST-3, stress and mood were ≥20% lower after playing away from home or losing (P < 0.05). Stress, mood and sleep were ≥12% worse after playing against a higher-level opposition at POST-1. Coaches need to be aware that match location, match result and the quality of the opposition can influence post-match wellbeing, irrespective of match load.

Effects Of Inorganic Nitrate And Nitrite Consumption On Cognitive Function And Cerebral Blood Flow: A Systematic Review And Meta-Analysis Of Randomised Clinical Trials

ABSTRACT We conducted a systematic review and meta-analysis of randomized clinical trials examining the effect of inorganic nitrate or nitrite supplementation on cognitive function (CF) and cerebral blood flow (CBF). Two databases (PubMed, Embase) were searched for articles from inception until May 2017. Inclusion criteria were: randomized clinical trials; participants >18 years old; trials comparing a nitrate/nitrite intervention with a control. Thirteen and nine trials were included in the meta-analysis to assess CF and CBF, respectively. Random-effects models were used and the effect size described as standardized mean differences (SMDs). A total of 297 participants (median of 23 per trial) were included for CF; 163 participants (median of 16 per trial) were included for CBF. Nitrate/nitrite supplementation did not influence CF (SMD +0.06, 95% CI: -0.06, 0.18, P = 0.32) or CBF under resting (SMD +0.14, 95% CI: -0.13, 0.41, P = 0.31), or stimulated conditions (SMD + 0.23, 95% CI: -0.11, 0.56, P = 0.19). The meta-regression showed an inverse association between duration of the intervention and CBF (P = 0.02) but no influence of age, BMI or dose (P < 0.05). Nitrate and nitrite supplementation did not modify CBF or CF. Further trials employing larger samples sizes and interventions with longer duration are warranted.

Beetroot juice is more beneficial than sodium nitrate for attenuating muscle pain after strenuous eccentric-bias exercise

The aim of this study was to compare the effects of beetroot juice (BTJ) and a nitrate only drink (sodium nitrate; SN) on indices of exercise-induced muscle damage (EIMD). Thirty recreationally active males consumed either BTJ (n = 10), a nitrate-matched SN drink (n = 10), or an isocaloric placebo (PLA; n = 10) immediately and at 24 and 48 h after performing 100 drop jumps. To assess muscle damage, maximal isometric voluntary contractions (MIVCs), countermovement jumps (CMJs), pressure-pain threshold (PPT), creatine kinase (CK), and high-sensitivity C-reactive protein (hs-CRP) were measured before, immediately after and at 24, 48, and 72 h following the drop jumps. BTJ and SN increased serum nitric oxide, which peaked at 2 h post-ingestion (136 ± 78 and 189 ± 79 μmol·L-1, respectively). PPT decreased in all groups postexercise (P = 0.001), but was attenuated with BTJ compared with SN and PLA (P = 0.043). PPT was 104% ± 26% of baseline values at 72 h after BTJ, 94% ± 16% after SN, and 91% ± 19% after PLA. MIVC and CMJ were reduced following exercise (-15% to 25%) and did not recover to baseline by 72 h in all groups; however, no group differences were observed (P > 0.05). Serum CK increased after exercise but no group differences were present (P > 0.05). hsCRP levels were unaltered by the exercise protocol (P > 0.05). These data suggest that BTJ supplementation is more effective than SN for attenuating muscle pain associated with EIMD, and that any analgesic effects are likely due to phytonutrients in BTJ other than nitrate, or interactions between them.

Countermovement Jump Recovery in Professional Soccer Players Using an Inertial Sensor

PURPOSE The purpose of this study was to assess the utility of an inertial sensor for assessing recovery in professional soccer players. METHODS In a randomized, crossover design, 11 professional soccer players wore shorts fitted with phase change material (PCM) cooling packs or uncooled packs (control) for 3 h after a 90 minute match. Countermovement jump (CMJ) performance was assessed simultaneously with an inertial sensor and an optoelectric system, pre match, and 12, 36 and 60 h post match. Inertial sensor metrics were flight height, jump height, low force, countermovement distance, force at low point, rate of eccentric force development, peak propulsive force, maximum power, and peak landing force. The only optoelectric metric was flight height. CMJ decrements, and effect of PCM cooling were assessed with repeated measures ANOVA. Jump heights were also compared between devices. RESULTS For the inertial sensor data there were decrements in CMJ height on the days after matches (88±10% of baseline at 36 h P=0.012, effect size 1.2, for control condition) and accelerated recovery with PCM cooling (105±15% of baseline at 36 h, P=0.018 vs. control, effect size 1.1). Flight heights were strongly correlated between devices (r=0.905, P<0.001) but inertial sensor values were 1.8±1.8 cm lower (P=0.008). Low force during countermovement was increased (P=0.031) and landing force was decreased (P=0.043) after matches, but neither were affected by the PCM cooling intervention. Other CMJ metrics were unchanged after matches. CONCLUSIONS This small portable inertial sensor provides a practical means of assessing recovery in soccer players.