Tomasz Huzarski
New York Academy of Medicine
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Featured researches published by Tomasz Huzarski.
Journal of Clinical Oncology | 2015
Honglin Song; Ed Dicks; Susan J. Ramus; Jonathan Tyrer; Maria P. Intermaggio; Jane Hayward; Christopher K. Edlund; David V. Conti; Patricia Harrington; Lindsay Fraser; Susan Philpott; Christopher N. G. Anderson; Adam Rosenthal; Aleksandra Gentry-Maharaj; David Bowtell; Kathryn Alsop; Mine S. Cicek; Julie M. Cunningham; Brooke L. Fridley; Jennifer Alsop; Mercedes Jimenez-Linan; Estrid Høgdall; C Hogdall; Allan Jensen; Susanne Kriiger Kjaer; Jan Lubinski; Tomasz Huzarski; Anna Jakubowska; Jacek Gronwald; Samantha Poblete
PURPOSEnThe aim of this study was to estimate the contribution of deleterious mutations in the RAD51B, RAD51C, and RAD51D genes to invasive epithelial ovarian cancer (EOC) in the population and in a screening trial of individuals at high risk of ovarian cancer.nnnPATIENTS AND METHODSnThe coding sequence and splice site boundaries of the three RAD51 genes were sequenced and analyzed in germline DNA from a case-control study of 3,429 patients with invasive EOC and 2,772 controls as well as in 2,000 unaffected women who were BRCA1/BRCA2 negative from the United Kingdom Familial Ovarian Cancer Screening Study (UK_FOCSS) after quality-control analysis.nnnRESULTSnIn the case-control study, we identified predicted deleterious mutations in 28 EOC cases (0.82%) compared with three controls (0.11%; P < .001). Mutations in EOC cases were more frequent in RAD51C (14 occurrences, 0.41%) and RAD51D (12 occurrences, 0.35%) than in RAD51B (two occurrences, 0.06%). RAD51C mutations were associated with an odds ratio of 5.2 (95% CI, 1.1 to 24; P = .035), and RAD51D mutations conferred an odds ratio of 12 (95% CI, 1.5 to 90; P = .019). We identified 13 RAD51 mutations (0.65%) in unaffected UK_FOCSS participants (RAD51C, n = 7; RAD51D, n = 5; and RAD51B, n = 1), which was a significantly greater rate than in controls (P < .001); furthermore, RAD51 mutation carriers were more likely than noncarriers to have a family history of ovarian cancer (P < .001).nnnCONCLUSIONnThese results confirm that RAD51C and RAD51D are moderate ovarian cancer susceptibility genes and suggest that they confer levels of risk of EOC that may warrant their use alongside BRCA1 and BRCA2 in routine clinical genetic testing.
British Journal of Cancer | 2002
A Jakubowska; K Nej; Tomasz Huzarski; Rodney J. Scott; J. Lubinski
Stomach cancer ranks second to lung cancer in the global cancer burden. It is estimated that 25% of families meeting the criteria for hereditary diffuse gastric carcinoma (HDCG) will have germline mutations in the E-cadherin gene. Evidence suggests that stomach cancer might also be a malignant manifestation of other inherited predispositions to disease. Recently, it has been reported that the incidence of stomach cancer is significantly increased in BRCA2 gene mutation carriers. We analysed by direct sequencing the BRCA2 gene in 29 breast cancer patients derived from 29 families with an aggregation of at least one female breast cancer diagnosed before the age of 50 years and one male stomach cancer diagnosed before the age of 55 years. In all but one of these families at least one additional relative was also affected by a malignant tumour. We identified three frameshift mutations and three sequence variants – potentially missense mutations, in six unrelated patients representing 20.7% (six out of 29) of the families investigated. Our results confirm that BRCA2 gene mutations are also associated with familial aggregations of not only breast but also of stomach cancer. In comparison to the number of cancers expected in the study population compared to the general population there is an over-representation of several cancers with significant confidence intervals to suggest that the associations are real and not a selection artefact.
European Journal of Human Genetics | 2003
Anna Jakubowska; Rodney J. Scott; Janusz Menkiszak; Jacek Gronwald; Tomasz Byrski; Tomasz Huzarski; Bohdan Górski; Cezary Cybulski; Tadeusz Dębniak; Elzbieta Kowalska; Teresa Starzyńska; Małgorzata Ławniczak; Steven A. Narod; Jan Lubinski
Germ-line mutations in the BRCA2 gene are associated with a wide range of cancer types, including the breast, ovary, pancreas, prostate and melanoma. In this study, we evaluated the importance of a family history of stomach cancer in predicting the presence of a BRCA2 mutation in Polish patients with ovarian cancer. A BRCA2 mutation was found in eight of 34 women with ovarian cancer and a family history of stomach cancer versus three of 75 women with ovarian cancer and a family history of ovarian cancer, but not of stomach cancer (odds ratio=7.4; 95% CI 1.8–30; P=0.004). The results of this study suggest that, in the Polish population, the constellation of ovarian and stomach cancer predicts the presence of a germ-line BRCA2 mutation and confirms that stomach cancer is part of the spectrum of BRCA2 mutations. It is expected that the penetrance of BRCA2 mutations for stomach cancer will vary from country to country, reflecting local environmental and lifestyle factors.
Clinical Genetics | 2005
G Kurzawski; Janina Suchy; Marcin Lener; Ewa Kłujszo-Grabowska; Józef Kładny; Krzysztof Safranow; K Jakubowska; A Jakubowska; Tomasz Huzarski; Tomasz Byrski; Tadeusz Dębniak; Cezary Cybulski; Jacek Gronwald; Oleg Oszurek; Dorota Oszutowska; Elsbieta Kowalska; S Góźdź; S Niepsuj; Ryszard Słomski; Andrzej Plawski; A Łącka-Wojciechowska; Andrzej Rozmiarek; Ł Fiszer-Maliszewska; M Bębenek; Sorokin D; Mm Sąsiadek; A. Stembalska; Z Grzebieniak; Ewa Kilar; M Stawicka
Germline mutations in the DNA mismatch repair genes MSH2 and MLH1 account for a significant proportion of hereditary non‐polyposis colorectal cancer (HNPCC) families. One approach by which development of an efficient DNA‐testing procedure can be implemented is to describe the nature and frequency of common mutations in particular ethnic groups. Two hundred and twenty‐six patients from families matching the Amsterdam II diagnostic criteria or suspected HNPCC criteria were screened for MSH2 and MLH1 germline mutations. Fifty different pathogenic mutations were found, 25 in MSH2 and 25 in MLH1. Twenty‐four of these had not previously been described in other populations. Among our 78 families with MSH2 or MLH1 mutations, 54 (69.2%) were affected by recurrent mutations including 38 found at least twice in our own series. Two of the most frequent alterations were a substitution of A to T at the splice donor site of intron 5 of MSH2 and a missense change (A681T) of MLH1 found in 10 and eight families, respectively. Among large deletions detected by the multiplex ligation‐dependent probe amplification assay, exon 9 deletions in the MSH2 gene were found in two families. Our results indicate that a screening protocol specific for the Polish population that is limited to the detection of all reported mutations will result in the identification of the majority of changes present in MLH1 and MSH2 genes in Polish HNPCC kindreds.
British Journal of Cancer | 2009
Jacek Gronwald; Cezary Cybulski; W Piesiak; J Suchy; Tomasz Huzarski; Tomasz Byrski; Bohdan Górski; Tadeusz Dębniak; M Szwiec; D Wokolowczyk; M Matuszewski; Ping Sun; J. Lubinski; Steven A. Narod
It is important to have accurate knowledge of the range of cancers associated with various CHEK2 mutations, and of the lifetime risks of cancer associated with each. We wished to establish the relationship between family history, mutation type and cancer risk in families with a CHEK2 mutation. We obtained a blood sample and pedigree information from 2012 unselected women with breast cancer, from 2007 men with prostate cancer and from 1934 patients with colon cancer, from hospitals throughout Poland. Genetic testing was carried out for four founder CHEK2 mutations on all 5953 specimens and 533 carriers were identified. We estimated the risk to age 75 for any cancer in the 2544 first-degree relatives to be 22.3%. After adjusting for mutation type, the risk of breast cancer was much higher among relatives of probands with breast cancer than among relatives of patients with prostate or colon cancer (HR=3.6; 95% CI=2.1–6.2; P=0.0001). Similarly, the risk of prostate cancer was higher among relatives of probands with prostate cancer than among relatives of patients with breast or colon cancer (HR=4.4; 95% CI=2.2–8.7; P=0.0001) and the risk of colon cancer was higher among relatives of probands with colon cancer than among relatives of patients with prostate or breast cancer (HR=4.2; 95% CI=2.4–7.8; P=0.0001). These analyses suggest that the risk of cancer in a carrier of a CHEK2 mutation is dependent on the family history of cancer.
Archive | 2015
Katarzyna Jaworska-Bieniek; Marcin Lener; Magdalena Muszyńska; Pablo Serrano-Fernández; Grzegorz Sukiennicki; Katarzyna Durda; Tomasz Gromowski; Satish Gupta; Józef Kładny; Anna Wiechowska-Kozłowska; Tomasz Grodzki; Ewa Jaworowska; J. Lubinski; Barbara Górecka-Szyld; Grażyna Wilk; Tomasz Huzarski; Tomasz Byrski; Cezary Cybulski; Jacek Gronwald; Tadeusz Dębniak; Olgierd Ashuryk; Aleksandra Tołoczko-Grabarek; Antoni W. Morawski; Rodney J. Scott; Anna Jakubowska; Jan Lubinski
Selenium is a trace element that is an essential component of many enzymes that play an important role in several major metabolic pathways and ameliorate environmental insult, including the antioxidant defense system, the immune system and functioning of the thyroid gland. There is a range of serum selenium levels associated, with the lowest rate of all causing mortality – the levels should be maintained at an optimal level and be neither too low nor too high. Selenium intake varies, largely based on the selenium content of food. The mean level of selenium in the population varies considerably between countries. Thus, for some countries selenium supplementation should be considered, whereas for others it could be contraindicated. Recent meta-analyses indicate unequivocally that selenium supplementation of people with low initial serum selenium levels decreases the incidence of cancer incidence by ∼35% and of cancer mortality by almost 50%. Additionally, it appears that in Se-deficient countries selenium levels may be a useful marker to select patients for cancer surveillance using for example computerized tomography for detection of the earliest stages of bronchial malignancy and colonoscopy for detection of the early stage colorectal cancer.
Journal of Clinical Oncology | 2016
Jacek Gronwald; Tomasz Byrski; Tomasz Huzarski; Rebecca Dent; V. Bielicka; D. Zuziak; Rafal Wisniowski; Jan Lubinski; Steven A. Narod
Human Mutation | 2001
Anna Jakubowska; Bohdan Górski; Tomasz Byrski; Tomasz Huzarski; Jacek Gronwald; Janusz Menkiszak; Cezary Cybulski; Tadeusz Dębniak; Piotr Hadaczek; Rodney J. Scott; Jan Lubinski
Archive | 2005
Cezary Cybulski; Jan Lubinski; Bohdan Górski; Bartlomiej Gliniewicz; Andrzej Sikorski; Tomasz Huzarski; Tadeusz Dębniak; Jacek Gronwald; Tomasz Byrski
Journal of Clinical Oncology | 2009
Tomasz Byrski; M. Foszczynska-Kloda; Tomasz Huzarski; Rebecca Dent; Jacek Gronwald; Cezary Cybulski; Tadeusz Dębniak; Bohdan Górski; Jan Lubinski; Steven A. Narod