Tomio Onuma

Identification of homozygous lipoprotein lipase gene mutation in a woman with recurrent aggravation of hypertriglyceridaemia induced by pregnancy

Shigeki Suga, Naoki Tamasawa, Ichiro Kinpara, Hiroshi Murakami, Nobuhiko Kasai, Tomio Onuma, Yasuyuki Ikeda, Atsuko Takagi, Toshihiro Suda (Hirosaki University School of Medicine, Aomori; and National Cardiovascular Center Research Institute, Osaka, Japan). Identification of homozygous lipoprotein lipase gene mutation in a woman with recurrent aggravation of hyperglyceridaemia induced by pregnancy (Case Report). J Intern Med 1998; 243: 317–21.

The effect of a new oral hypoglycemic drug, CS-045, on glucose tolerance and serum lipids in nonobese japanese patients with non-insulin-dependent diabetes mellitus: A pilot study

Abstract This study examined the effects of a new oral hypoglycemic drug, CS-045, on glucose tolerance and serum lipids in 10 nonobese patients with non-insulin-dependent diabetes mellitus (NIDDM). Plasma glucose levels before and at 2, 3, 4, 6, 8, 10, 15, 20, and 30 minutes after administration of the intravenous glucose tolerance test (IVGTT) were significantly ( P P P P P

Lipid composition of platelets in patients with non-insulin-dependent diabetes mellitus: studies before and after treatment of diabetes.

The study was designed to investigate whether impaired composition of platelet lipids in untreated diabetic patients improved after diabetic treatment. Fourteen untreated patients with non‐insulin‐dependent diabetes mellitus (NIDDM) and 15 healthy control subjects were studied. In the diabetic patients, the ratio of free cholesterol to phospholipid (FC/PL) in platelets of 0.33 ± 0.02 (mean ± SEM) at pre‐treatment, which was statistically (p < 0.05) higher than that of 0.26 ± 0.02 in control subjects, was significantly decreased to the value of 0.29 ± 0.02 (p < 0.01) after insulin therapy. Platelet FC level of 9.77 ± 0.77 μg 10−8 cells pre‐treatment was significantly (p < 0.01) reduced to the value of 7.72 ± 0.38 μg 10−8 cells post‐treatment. Platelet PL level showed no significant changes after the treatment. There was a significantly (p < 0.01) positive correlation between the decrease in FC/PL of platelets and that in haemoglobin A1c (HbA1c) after treatment for diabetes (rs = −0.729). These results indicate that the impaired lipid composition in platelets can be improved after an adequate glycaemic control in patients with NIDDM.

Acid cholesteryl ester hydralase activity of mononuclear leukocytes in patients with non-insulin-dependent diabetes mellitus: studies before and after treatment of diabetes

The change of acid cholesteryl ester hydrolase activity in mononuclear leukocyte following treatment of diabetes mellitus was studied in 21 patients with non-insulin-dependent diabetes mellitus (NIDDM). Enzyme activity before treatment in the patients was significantly lower than that in 14 age-matched healthy subjects (1.20 +/- 0.15; mean +/- S.E. vs. 2.20 +/- 0.17 nmol/mg protein/h, P less than 0.01). Enzyme activity before treatment in the patients was significantly increased (P less than 0.05) after 4-8 weeks of treatment. However, enzyme activity of 1.43 +/- 0.14 nmol/mg protein/h observed after treatment in the patients was significantly lower (P less than 0.01) than that in the healthy subjects. There was a significant negative correlation between enzyme activity before treatment and the increase in enzyme activity following treatment (rs = -0.555, P less than 0.01, n = 21). These results indicate that low level of enzyme activity may be insufficiently improved by the treatment of diabetes, and the risk for the development of atherosclerosis as viewed from the enzyme activity may persist even after the treatment in NIDDM.

Delayed somatosensory conduction in acute painful neuropathy of diabetes.

We examined the peripheral–central sensory conduction by using somatosensory evoked potential (SEP) in a 48 year old diabetic patient with acute painful neuropathy. The sural, ulnar and median sensory nerve conduction and SEP elicited by wrist stimulation showed no abnormalities, nevertheless, the tibial nerve SEP revealed absent spinal N19 and a remarkable delay of the cortical arrival time. These findings suggest involvement of the dorsal roots or the dorsal column in the acute painful neuropathy of diabetes.

High incidence of diabetic nephropathy in non-insulin-dependent diabetic patients with heterozygous familial hypercholesterolemia

Abstract The present study was performed to assess the influence of hyperlipidemia on the appearance and development of diabetic nephropathy in patients with non-insulin-dependent diabetes mellitus (NIDDM). This was done by comparing the incidence of nephropathy in 35 diabetic patients with heterozygous familial hypercholesterolemia (FH) with the incidence of nephropathy in 165 patients without FH. There was no significant difference in sex, age, body mass index, or mean duration of diabetes between the two groups. Both groups received similar treatment for diabetes. No significant difference in mean hemoglobin A 1c levels or prevalence of hypertension was noted between the two groups. Total serum cholesterol and triglyceride levels were significantly higher ( P P

Enhanced accumulation of cholesterol ester in macrophages from diabetic rats incubated with oxidized low-density lipoprotein

Abstract The metabolism of cholesterol ester (CE) was studied in peritoneal macrophages taken from rats with streptozocin-induced diabetes. Levels of CE and synthesized [ 14 C]-cholesterol oleate did not differ significantly between the macrophages from diabetic and control rats incubated with low-density lipoprotein (LDL). However, when macrophages were incubated with Cu 2+ -induced oxidized LDL, CE levels were significantly higher in diabetic rats than in control rats (3.03± 0.09 μg/10 6 cells versus 2.66±0.09 μg/10 6 cells; P 14 C]-cholesterol oleate did not differ significantly between the two groups of rats. The findings suggest that CE accumulates in macrophages in the presence of diabetes mellitus and may play a role in the development of atherosclerosis in diabetes mellitus.