Tomoko Maruyama
Shizuoka University
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Tomoko Maruyama.
Microbial Ecology | 2003
Tomoko Maruyama; Kenji Kato; Atsushi Yokoyama; T. Tanaka; Akira Hiraishi; Ho-Dong Park
To reveal the process of degradation of hepatotoxic microcystin produced in Microcystis cells during the Microcystis bloom period, we used fluorescence in situ hybridization (FISH) to analyze the population dynamics of microcystin-degrading bacteria in Microcystis mucilage. We designed and applied an oligonucleotide probe targeted to the 16S rRNA sequence of strain Y2 of a microcystin-degrading bacterium (MCD-bacterium), which was isolated from Lake Suwa, Japan. In both the 1998 and 1999 tests, FISH clearly showed that MCD-bacteria existed in the mucilage and that, when a high concentration of cell-bound microcystin was detected, MCD-bacteria exceeded 10% of the sum of bacteria hybridized with group-specific probes. The concentration of MCD-bacteria was highest in summer 1998, when a toxic species, M. viridis, was dominant. There was a high correlation between the number of MCD-bacteria in the mucilage and the concentration of cell-bound microcystin in the lake. Our results suggest that MCD-bacteria responded to changes in the concentration of microcystin and degraded the microcystin when it was released from Microcystis cells. We also analyzed changes in the bacterial community structure associated with the Microcystis colonies by using domain- and group-specific oligonucleotide probes. Changes in the concentrations of the Cytophaga/Flavobacterium group and 8-Proteobacteria, which can degrade macromolecules derived from Microcystis cells, were synchronized with changes in the concentration of Microcystis. The results not only suggest the significant role of MCD-bacteria in detoxification, but also demonstrate a possible sequence of degradation from Microcystis cells to microcystin maintained in the cell, which is then carried out by bacterial consortia in the mucilage.
Geomicrobiology Journal | 2008
Kazuyo Nagaosa; Kenji Kato; Tomoko Maruyama; Nihal Welikala; Yohei Yamashita; Yui Saito; Danielle Fortin; Kenji Nanba; Iku Miyasaka; Sakae Fukunaga
To elucidate bacterial population dynamics in an aquifer, we attempted to reveal the impact of protozoan grazing on bacterial productivity and community structure by an in situ incubation experiment using a diffusion chamber. The abundance and vertical distribution of bacteria and protozoa in the aquifer were revealed using wells that were drilled in a sedimentary rock system in Itako, Ibaraki, Japan. The water column in the wells possessed aerobic and anaerobic layers. Active bacterial populations under the grazing pressure of protozoa were revealed through in situ incubation with grazer eliminating experiment by the filtration. On August 19, 2003, the total number of bacteria (TDC) decreased from 1.5 × 106 cells ml− 1 at 2.2 m depth to 3.0 × 105 cells ml− 1 at 10 m depth. The relative contribution of the domain Bacteria to TDC ranged between 63% and 84%. Protozoa existed at a density of 4.2 × 104 to 1.9 × 105 cells ml− 1 in both aerobic and microaerobic conditions. A grazing elimination experiment in situ for 6 days brought about clearly different bacterial community profiles between the 2.2 m and 10 m samples. The bacterial composition of the initial community was predominantly β- and γ -proteobacteria at 2.2 m, while at 10 m β-, α - and γ -proteobacteria represented 56%, 26% and 13% of the community, respectively. The distribution of bacterial abundance, community composition and growth rates in the subsurface were influenced by grazing as well as by geochemical factors (dissolved oxygen and concentrations of organic carbon, methane and sulfate). Results of the in situ incubation experiment suggested that protozoan grazing contributes significantly to bacterial population dynamics.
Subseafloor Biosphere Linked to Hydrothermal Systems,TAIGA Concept/Springer | 2015
Hiroyuki Yamamoto; Tomoko Maruyama; László G.-Tóth; Kenji Kato; Yasuo Furushima; Naoto Taira; Yoshiaki Maeda; Kiminori Shitashima
The doubling time of indigenous bacteria in mixing-zone of hydrothermal fluid and seawater was determined using a diffusion chamber unit deployed on the field of Hatoma Knoll (24° 51.50′N, 123° 50.50′ E), which is a submarine volcano located on southern Okinawa Trough. The diffusion chamber is a reliable tool to incubate and to directly measure the microbial growth under in situ condition of deep-sea, although an operation of submersible and a complicated preparation of seed water became the technical constraints. The doubling time at non-vent site distant from active vent site was estimated from 86 to 110 h, while at active vent sites more rapid doubling time, 21–32 h, were estimated. A potential sulfur-oxidizing bacteria belonging to Epsilonproteobacteria dominated the population grew in the chambers, which were incubated using the plume water obtained from the mixing zone between the vent fluid and seawater, and Bathymodiolus colony, while no detection of Gammaproteobacteria. The methane-oxidizing bacteria were detected only from gill and digestive tract of Bathymodiolus platifrons, and could not be detected from the chamber, although the chamber was placed on Bathymodiolus colony. The results of this study suggested that chemolithoautotrophic growth near by the hydrothermal vent is sustained by the rapid doubling time of Epsilonproteobacteria using chemical species dissolved in fluid and provides the chemoautotrophic product to deep-sea benthopelagic community, as well as a microbial products in hydrothermal vent plume.
Environmental Toxicology | 2001
Ho-Dong Park; Youhei Sasaki; Tomoko Maruyama; Eiji Yanagisawa; Akira Hiraishi; Kenji Kato
International Journal of Systematic and Evolutionary Microbiology | 2006
Tomoko Maruyama; Ho-Dong Park; Kazuhiko Ozawa; Yoshinori Tanaka; Tatsuo Sumino; Koei Hamana; Akira Hiraishi; Kenji Kato
Microbes and Environments | 2005
Akira Hiraishi; Noriko Sakamaki; Hideki Miyakoda; Tomoko Maruyama; Kenji Kato; Hiroyuki Futamata
Microbes and Environments | 2007
Takayuki Takenaka; Tomokazu Tashiro; Ayumi Ozaki; Hitomi Takakubo; Yutaka Yamamoto; Tomoko Maruyama; Kazuyo Nagaosa; Hiroyuki Kimura; Kenji Kato
Microbes and Environments | 2004
Tomoko Maruyama; Kenji Kato; Ho-Dong Park
Archive | 2003
Tomoko Maruyama; Kenji Kato; Atsushi Yokoyama; T. Tanaka; Akira Hiraishi
한국미생물학회 학술대회논문집 | 2007
Kenji Kato; László G.-Tóth; Tomoko Maruyama; Miyuki Nishijima; Hiroyuki Kimura; Kazuyo Nagaosa; Kenji Nanba; Katsunori Fujikura; Hiroyuki Yamamoto