Tong Shangguan
Princeton University
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Featured researches published by Tong Shangguan.
Biochimica et Biophysica Acta | 1998
Tong Shangguan; Charles Pak; Shaukat Ali; Andrew S. Janoff; Paul Meers
N-acyl phosphatidylethanolamines (NAPEs) are natural lipid components of many organisms. N-acylation of unsaturated phosphatidylethanolamines with a saturated fatty acid converts them from non-lamellar organizing lipids into lamellar organizing, acidic lipids which can interact with cations and potentially return to non-lamellar structures. These special properties make NAPEs candidates for fusogens. We tested the fusogenicity of one of the NAPEs, N-dodecanoyl-di-oleoylphosphatidylethanolamine (N-C12-DOPE) mixed with dioleoylphosphatidylcholine (DOPC) in liposomes. Binding and fusion to erythrocyte ghosts in the presence of 3 mM Ca2+ required at least 60 mol% of N-C12-DOPE. Fusion was not observed when phosphatidylglycerol or phosphatidylserine was substituted for N-C12-DOPE, indicating specificity for properties of this lipid. Binding of N-C12-DOPE/DOPC (70:30) liposomes required 1 mM Ca2+ while 1.25 mM Ca2+ and Mg2+ were sufficient for lipid mixing and delivery of encapsulated dextrans to erythrocyte ghosts. These liposomes also bound and possibly mixed lipid with nucleated U-937 cells in a Ca2+ -and endocytosis-dependent manner. Low pH-dependent fusion with ghosts was observed in the absence of any divalent cation, indicating that fusion with U-937 cells could result after endocytosis into the acidic endosomes. The possible mechanisms for N-C12-DOPE mediated binding and fusion and the potential application of these liposomes as delivery vehicles for therapeutic agents are discussed.
Archive | 1997
Paul Meers; Tong Shangguan; Shaukat Ali; Andrew S. Janoff; Charles Pak
Archive | 2000
Paul Meers; Tong Shangguan; Donna Cabral-Lilly; Andrew S. Janoff; Patrick L. Ahl
Biochimica et Biophysica Acta | 2005
Alla Polozova; Xingong Li; Tong Shangguan; Paul Meers; Daniel R. Schuette; Nozomi Ando; Sol M. Gruner; Walter Perkins
Archive | 2003
Xingong Li; Tong Shangguan; Alla Polozova; Paul Meers; Walter Perkins
Archive | 2000
Patrick L. Ahl; Donna Cabral-Lilly; Andrew S. Janoff; Paul Meers; Tong Shangguan
Archive | 2000
Patrick L. Ahl; Donna Cabral-Lilly; Ravi K. Erukulla; Andrew S. Janoff; Paul Meers; Tong Shangguan
Archive | 2000
Paul Meers; Tong Shangguan; Donna Cabral-Lilly; Andrew S. Janoff; Patrick L. Ahl
Archive | 2000
Paul Meers; Tong Shangguan; Donna Cabral-Lilly; Andrew S. Janoff; Patrick L. Ahl
Archive | 1997
Paul Meers; Tong Shangguan; Shaukat Ali; Andrew S. Janoff; Charles Pak