Tongchun Mao

Role of serum amyloid P in skin graft survival and wound healing in burned patients receiving skin grafts.

BACKGROUND Recent studies in animal models suggest that serum amyloid P (SAP) can affect burn wound healing. However, the role of SAP in a clinical setting remains unknown. METHODS We enrolled 88 patients with third degree burn wounds. All the patients were candidates for auto-skin graft procedure using stamp skin graft. The complete graft healing time and the number of survived grafts were recorded. Serum SAP levels were assessed 1 day before operation. RESULTS There was no significant difference in SAP level between controls and patients. There were no significant differences noted among the patients with different burn surface area. However, when the patients in each group were stratified by SAP levels, the mean complete healing time of grafted wound and the mean numbers of survived skin grafts were significantly different. Spearmans analyses showed that the serum SAP levels negatively correlated with the complete wound healing time and mean numbers of survived skin grafts. Logistic regression analysis showed that the serum SAP levels and mean numbers of survived skin grafts were potent independent factors contributing to wound healing. CONCLUSIONS The results of this study suggest that the serum SAP levels may be an easy detected predictor for the healing of burn wounds.

A simple and practical method for axillary osmidrosis resection.

1748-6815/

Effect of microtubule-associated protein-4 on epidermal cell migration under different oxygen concentrations

-seefrontmattera2009BritishAssociationofPlastic,Reconstruc doi:10.1016/j.bjps.2009.11.012 of the axillary area. While many new techniques have proven effective (e.g., laser, suction-assisted liposuction), their application is limited by the need for specialized instrumentation. Here we present a simple, practical and radical surgical method based on limited incision. First, the area to be undermined is marked and an incision line of 2 cm is cut into the subdermal fat tissue. We evert the flap strongly with index finger, dragging the skin flap with two

Genetic association between p53 codon 72 polymorphism and risk of cutaneous squamous cell carcinoma

After skin trauma, regional epidermal cell migration mediates the re‐epithelialization of the wound surface, which is an important step for wound healing, yet the underlying molecular regulatory mechanism is unclear. In the current study, HaCaT cells were maintained under different oxygen concentrations (1%, 21%, 40% and 65%). Technologies including immunofluorescence staining, wound scratch, transwell invasion, western blot and low‐expression lentiviral vector were utilized to observe the changes in microtubule dynamics and the microtubule‐associated protein (MAP)4 expression. MAP4s effect on cell migration under different oxygen concentrations was also studied. The results showed that under hyperoxic (40% and 65%) and hypoxic (1%) conditions, HaCaT cells were able to regulate cell microtubule dynamics by MAP4, thus promoting cell migration. On the other hand, MAP4 silencing through targeted shRNA attenuated HaCaT cell migration under the above oxygen concentrations. These results imply that MAP4 plays an important role in epidermal cell migration under different oxygen concentrations.