Toshiji Kobayashi
Keio University
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Publication
Featured researches published by Toshiji Kobayashi.
Journal of UOEH | 1991
Masahisa Honda; Masako Tsuchihashi; Minoru Hayashi; Masao Arai; Toshiji Kobayashi
There are several problems in measuring the activities of urinary enzymes, such as alkaline phosphatase (ALP), leucine aminopeptidase (LAP), and gamma-glutamyl transpeptidase (gamma-GTP) which are derived from renal tubulus. The purpose of the present study is to clarify the basic methodological problems in measuring these enzyme activities. Our results indicate that it is not necessary to dialyze urine when determining these three enzymes, except for alkaline phosphatase activity measured by the Kind-King method. Because of contamination of urine by bacteria and cell elements, the enzyme activity is influenced by centrifugal conditions that depend on time and speed. Our results propose that it is necessary, at least, to centrifuge at 3000 rpm for 10 minutes to obtain satisfactory data. Generally, a 24 hour urine sample instead of spot urine sample should be used for measuring the enzyme activity. However if correcting gamma-GTP activity by creatinine, even a spot urine sample may be used for clinical use.
Journal of UOEH | 1988
Tadao Tanabe; Toshiji Kobayashi; Shin-ichi Yoshida; Yasuo Mizuguchi
Susceptibilities of Gram-negative bacterial strains isolated at the University Hospital in 1987 to six beta-Lactam antibiotics (azthreonam cefmetazole, ceftizoxime, latamoxef, aminobenzyl-penicillin and piperacillin) and two aminoglycoside antibiotics (gentamicin and amikacin) were examined by agar dilution and the agar diffusion method. Results obtained from both methods correlated well in most of the strains. Most of the strains belonging to Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae and Proteus mirabilis were sensitive to the beta-lactam antibiotics except aminobenzyl-penicillin and piperacillin. They were also sensitive to aminoglycoside antibiotics. Most strains of Citrobacter freundii, Enterobacter cloacae and Serratia marcescens were sensitive to azthreonam, latamoxef and aminoglycosides, but were less sensitive to other beta-lactams. Acinetobacter calcoaceticus was generally resistant to beta-lactams except ceftizoxime, but was sensitive to aminoglycosides. More than half of the strains of Pseudomonas aeruginosa were sensitive to azthreonam, piperacillin and aminoglycoside antibiotics and only latamoxef was active in Pseudomonas maltophilia.
Hepatology | 1989
Masao Arai; Shigeo Nakano; Fumio Okuno; Yoshiaki Hirano; Kazufumi Sujita; Toshiji Kobayashi; Hiromasa Ishii; Masaharu Tsuchiya
Archive | 1983
Jack Jellins; Toshiji Kobayashi
Journal of UOEH | 1989
Toshiji Kobayashi; Hiromi Shinozaki; Makoto Yomon; Minoru Hayashi; Masao Arai; Fujio Zeze; Osamu Koide
Journal of UOEH | 1985
Toshiji Kobayashi; Minoru Hayashi; Masao Arai
Hepatology | 1986
Masao Arai; Fumio Okuno; Yoshiaki Hirano; Toshiji Kobayashi; Hiromasa Ishii; Masaharu Tsuchiya
Journal of UOEH | 1983
Katsuji Kobori; Hirokazu Sakakibara; Katsuya Maruyama; Toshiji Kobayashi; Toshio Yamaki
Journal of UOEH | 1987
Tetsuro Iwami; Yasuhiro Kajiwara; Minoru Yamagishi; Tadao Tanabe; Kiyotaka Uemura; Yohko Ariyoshi; Toshiji Kobayashi
Journal of UOEH | 1980
Toshiji Kobayashi; Minoru Hayashi
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University of Occupational and Environmental Health Japan
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