Toshikazu Nomizu
Niigata University
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Publication
Featured researches published by Toshikazu Nomizu.
Plant Cell Tissue and Organ Culture | 2004
Toshikazu Nomizu; Yoshiji Niimi; Dong-Sheng Han
An anther culture technique for the production of haploid plants was developed in Hepatica nobilis. Embryos with bipolar meristem regions were induced from microspores within the cultured anthers. Embryo formation was promoted by first culturing anthers on NN medium (Nitsch and Nitsch, 1969) supplemented with 1% activated charcoal (AC) at 5 or 35 °C for a few days and by then incubating them in the dark at 25 °C. Pre-culturing anthers at 35 °C for 4 days (thermal-shock treatment) led to the best embryo formation (45 embryos/Petri dish with 30 anthers). Plant regeneration was achieved by culturing the anther-derived embryos on NN medium without AC at 15 °C. Flow cytometric analysis of anther-derived embryos and chromosome counts in regenerated plants showed that they were haploid plants.
Scientia Horticulturae | 2006
Masaru Nakano; Toshikazu Nomizu; Keiko Mizunashi; Mami Suzuki; Shiro Mori; Sachiko Kuwayama; Megumi Hayashi; Hiroto Umehara; Eriko Oka; Hiroyuki Kobayashi; Megumi Asano; Shintaro Sugawara; Hiroki Takagi; Hiroyuki Saito; Masashi Nakata; Toshinari Godo; Yoshihiro Hara; Junji Amano
Scientia Horticulturae | 2010
Atsushi Matsumura; Toshikazu Nomizu; Noriyuki Furutani; Ken Hayashi; Yasuhiro Minamiyama; Yoshihiro Hase
Scientia Horticulturae | 2004
Toshikazu Nomizu; Yoshiji Niimi; Eriko Watanabe
Plant Biotechnology | 2010
Masaru Nakano; Junji Amano; Yusuke Watanabe; Toshikazu Nomizu; Mami Suzuki; Keiko Mizunashi; Shiro Mori; Sachiko Kuwayama; Dong-Sheng Han; Hiroyuki Saito; Hiromichi Ryuto; Nobuhisa Fukunishi; Tomoko Abe
Archive | 2011
Yoshikazu Tanaka; Noriko Nakamura; Hitoshi Kobayashi; Hiroaki Okuhara; Masayoshi Kondo; Yosuke Koike; Yosuke Hoshi; Toshikazu Nomizu
Horticultural Research (japan) | 2013
Yusuke Watanabe; Kazunori Miyajima; Toshikazu Nomizu; Masaru Nakano; Kazuo Ichimura
Archive | 2011
Yoshikazu Tanaka; Noriko Nakamura; Hitoshi Kobayashi; Hiroaki Okuhara; Masayoshi Kondo; Yosuke Koike; Yosuke Hoshi; Toshikazu Nomizu
Plant Growth Regulation | 2010
Masaru Nakano; Yusuke Watanabe; Toshikazu Nomizu; Mami Suzuki; Keiko Mizunashi; Shiro Mori; Junji Amano; Dong-Sheng Han; Hiroyuki Saito; Hiromichi Ryuto; Nobuhisa Fukunishi; Tomoko Abe
Journal of The Japanese Society for Horticultural Science | 2003
Toshikazu Nomizu; Yoshiji Niimi; Shunsaku Kasahara
