Toshimitsu Fukiharu

Ammonia fungi of iriomote Island in the southern Ryukyus, Japan and a new ammonia fungus,Hebeloma luchuense

Species composition and fruiting season of ammonia fungi were investigated in Iriomote Island of the southern Ryukyus, Okinawa Prefecture, Japan.Castanopsis andPinus forests were surveyed and 10 species of ammonia fungi were collected, including one new agaric species,Hebeloma luchuense sp. nov. This new fungus is characterized by having a rooting, squamulose-scaly stipe and cortinate veil and forms ectomycorrhizae withCastanopsis cuspidata var.sieboldii. Although the general mushroom season in theCastanopsis forest in Iriomote island was very short and restricted to summer, ammonia fungi were observed to fruit throughout the year in urea-treated plots.

Identification and symbiotic ability of Psathyrellaceae fungi isolated from a photosynthetic orchid, Cremastra appendiculata (Orchidaceae)

UNLABELLED PREMISE OF THE STUDY Photosynthetic orchids found in highly shaded forests are often mixotrophic, receiving part of their carbon energy via ectomycorrhizal fungi that had originally received carbohydrate from trees. A photosynthetic orchid, Cremastra appendiculata, is also found under highly shaded forest, but our preliminary data suggested that its associated fungi were not ectomycorrhizal. We tested whether their relation is an unusual example of a mixotrophic orchid associating with saprotrophic fungi by direct detection of fungal DNAs in conjunction with isolation of the fungus in pure culture and experimental inoculation of orchid seeds with the fungus. • METHODS For isolated mycobionts of C. appendiculata plants, two regions of nuclear ribosomal DNA, the internal transcribed spacer (ITS) and the large subunit (LSU), were sequenced, and fruiting bodies of the one isolate, SI1-1 were induced. In addition, two fungal isolates, SI1-1 and KI1-1, were grown in symbiotic cultures with C. appendiculata to verify their status as mycobionts. • KEY RESULTS In phylogenetic analyses, all isolates clustered with fungi belonging to Coprinellus in Psathyrellaceae of Agaricales. Phylogenetic analyses of these DNA sequences showed that five fungal isolates from C. appendiculata, including SI1-1 and two mycobionts isolated from the mycoheterotrophic orchid Epipogium roseum, have very similar ITS sequences. Isolate SI1-1 was identified as Coprinellus domesticus based on the morphological characteristics of the fruiting body. Isolates SI1-1 and KI1-1 induced seed germination of C. appendiculata as mycobionts. • CONCLUSIONS This report is the first of a mycorrhizal symbiosis between a fungus in Psathyrellaceae and a photosynthetic orchid, revealing a new pathway to full mycoheterotrophy and contributing to our understanding of the evolution of mycoheterotrophy.

Hebeloma radicosoides sp. nov., an agaric belonging to the chemoecological group ammonia fungi

Hebeloma radicosoides sp. nov. is described, illustrated and characterised. It superficially resembles H. radicosum in having a long-rooting stipe with a well-formed, membranous annulus and in having preference for animal wastes as its resources. Its pileus is yellower than that of H. radicosum, and it fruits after addition of urea to soil, whereas H. radicosum does not. It also fruits on various animal wastes whereas H. radicosum specifically fruits on mole or mouse middens. Unlike H. radicosum, H. radicosoides has no distinct odour. Hebeloma radicosoides also resembles Hebeloma luchuense and H. spoliatum in having a long-rooting stipe and in fruiting on urea-treated soil, but differs from them by having a yellowish scaly pileus and distinct, membranous annulus.

An analysis on the spatial distribution patterns of basidiocarps of Agaricales in a Castanopsis-dominated forest in Kyoto

Spatial distribution patterns of basidiocarps of Agaricales were studied in aCastanopsis-dominated forest in Kyoto. Sixty-seven species were recorded, of which 45 species and 99% of total dry weight were ectomycorrhizal fungi belonging to Amanitaceae, Cortinariaceae, Russulaceae, Boletaceae and Strobilomycetaceae. The data were analyzed statistically by using the m-m regression method. Three distribution patterns were recognized: aggregated, random and uniform. Although total basidiocarps were distributed randomly, basidiocarps of most species showed aggregated distributions, suggesting mycelium of ectomycorrhizal and saprotrophic fungi extend in a limited area in the soil. The degree of aggregation was different among species and this difference was suggested to reflect the difference of niche among the species.

Occurrence of Hebeloma vinosophyllum on the forest ground after decomposition of crow carcass

Hebeloma vinosophyllum (Basidiomycota, Agaricales), a member of the ammonia fungi, occurred on the ground in the close vicinity of the decomposed carcass of a jungle crow,Corvus macrorhynchos, in a forest dominated byQuercus spp. in Urawa, Saitama Pref., central Japan. This is the first report of an ammonia fungus occurring at the site of a decomposed wild bird carcass.

Morphology and comparative ecology of the fairy ring fungi, Vascellum curtisii and Bovista dermoxantha, on turf of bentgrass, bluegrass, and Zoysiagrass

We identified the causal fungi of fairy rings as Vascellum curtisii and Bovista dermoxantha on the turf of bentgrass, bluegrass, and Zoysiagrass. Observing the fairy rings developed in the turfgrass study site in Chiba City for 5 years from 1998, V. curtisii, producing sporophores during June and November except midsummer, formed darker-green rings than the adjacent turf and withered the three kinds of turf. B. dermoxantha produced sporophores exclusively in midsummer. The fungus formed dark green rings on the three kinds of turf but withered only bentgrass and bluegrass. The optimum mycelial growth temperature of V. curtisii was 30°C. Whereas that of B. dermoxantha ranged between 35° and 40°C. The infection test of the fungi to the seedlings revealed that V. curtisii damaged Zoysiagrass more severely than bentgrass and that B. dermoxantha was more injurious to bentgrass than Zoysiagrass.

Saprobic and ectomycorrhizal ammonia fungi in the Southern Hemisphere

Abstract The biogeographic distribution of ammonia fungi is reviewed using collection records of ammonia fungi, mainly obtained by artificial applications of urea in the field and in laboratory experiments. Distribution records are fragmentary at a global scale. However, generally speaking, the biogeographic distribution of ectomycorrhizal ammonia fungi appears to be more restricted than that of saprobic ammonia fungi. Those saprobic species of more restricted distribution are likely to have a more rigid substrate specificity. The biogeographic distribution of ectomycorrhizal ammonia fungi is likely to relate to the distribution of host mycorrhizal tree(s) based on the degree of host specificity. Patterns of biogeographic distribution of ammonia fungi can be categorised into six regional distribution types: ubiquitous, Northern Hemisphere, East Asia, East Asia and Oceania, Australia and New Zealand, and Australia endemic. Among the ammonia fungi, closely related counterpart species in each hemisphere are recognised.

Emericella qinqixianii, a new species from desert soil in China.

Emericella qinqixianii, a new species isolated from desert soil from Sanchakou, Aksu, Qiemo, Yuli, Yutian, and the Taklimakan desert 100 km inland from Minfeng, Xinjiang Province, China, is described and illustrated. It is characterized by grayish yellow to olive brown, non-ostiolate ascomata surrounded by hyaline to pale yellowish brown hülle cells, membranaceous peridium, prototunicate asci, and violet-brown, lenticular ascospores with two equatorial crests, smooth convex surfaces, and long filiform appendages. It hasAspergillus anamorph with biseriate aspergilla.

Coprinopsis neophlyctidospora sp. nov., a new ammonia fungus from boreal forests in Canada

Coprinopsis neophlyctidospora sp. nov. (Basidiomycota, Agaricales), collected in urea treated soil of boreal forests from Canada is described and illustrated. Its micromorphological features, phylogenetic analysis, and mating test delineate this taxon as a new species. In addition, its ecological characters also indicate it is a new ammonia fungus.

Dikaryotic arthroconidiation of Pleurotus subgenus Coremiopleurotus

This study was carried out to elucidate the poorly understood processes of arthroconidiation through coremium formation using Pleurotus cystidiosus subsp. abalonus. The coremia exclusively produced dikaryotic arthroconidia with the remnant of a clamp connection. Cells in the subapical zone of the hyphal bundle reduced their length by division before arthroconidiation. Approximately 400 000 arthroconidia were produced by a coremium in 1 day, with constant productivity over a 2-week period. Continuous cell extension and division in the coremium stipe supplied cells for arthroconidiation at the coremium apex, which is surrounded by a liquid droplet (coremioliquid). Maintenance of moisture with coremioliquid was necessary for arthroconidiation. The coremioliquid formation was performed by active liquid transportation in the hyphae, a process that was blocked by the microtubule depolymerization agent thiabendazole.