Toshinobu Miyamoto
Asahikawa Medical University
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Publication
Featured researches published by Toshinobu Miyamoto.
Advances in Urology | 2012
Toshinobu Miyamoto; Akira Tsujimura; Yasushi Miyagawa; Eitetsu Koh; Mikio Namiki; Kazuo Sengoku
Infertility is one of the most serious social problems facing advanced nations. In general, approximate half of all cases of infertility are caused by factors related to the male partner. To date, various treatments have been developed for male infertility and are steadily producing results. However, there is no effective treatment for patients with nonobstructive azoospermia, in which there is an absence of mature sperm in the testes. Although evidence suggests that many patients with male infertility have a genetic predisposition to the condition, the cause has not been elucidated in the vast majority of cases. This paper discusses the environmental factors considered likely to be involved in male infertility and the genes that have been clearly shown to be involved in male infertility in humans, including our recent findings.
Gynecologic and Obstetric Investigation | 1999
Hiroaki Hayashi; Yuji Yaginuma; Shinitsu Kitamura; Yuji Saitou; Toshinobu Miyamoto; Harumi Komori; Keiko Wada; Mutsuo Ishikawa
The purpose of this study was to evaluate bilateral oophorectomy in women over 50 years old found to have an adnexal mass using transvaginal ultrasonography (TVS) as a mass screening. With TVS a total of 23,451 women without symptoms were examined for ovarian cancer at annual screening for uterine cervical cancer. Two hundred fifty-eight women over 50 years old persistently had abnormal TVS results and 95 women gave informed consent for surgical tumor removal. In the 95 women operated, 7 malignant ovarian cancers were found. Especially adnexal masses which were thought to be benign were treated by laparoscopic surgery.
Journal of Obstetrics and Gynaecology Research | 2015
Toshinobu Miyamoto; Gaku Minase; Kimika Okabe; Hiroto Ueda; Kazuo Sengoku
Infertility is a serious social problem in advanced nations, with male factor infertility accounting for approximately half of all cases of infertility. Here, we aim to discuss our laboratory results in the context of recent literature on critical genes residing on the Y chromosome or autosomes that play important roles in human spermatogenesis.
Journal of Andrology | 2012
Hiroe Miyakawa; Toshinobu Miyamoto; Eitetsu Koh; Akira Tsujimura; Yasushi Miyagawa; Yasuaki Saijo; Mikio Namiki; Kazuo Sengoku
Genetic mechanisms have been implicated as a cause of some cases of male infertility. Recently, 10 novel genes involved in human spermatogenesis, including human SEPTIN12, were identified by expression microarray analysis of human testicular tissue. Septin12 is a member of the septin family of conserved cytoskeletal GTPases that form heteropolymeric filamentous structures in interphase cells. It is expressed specifically in the testis. Therefore, we hypothesized that mutation or polymorphisms of SEPTIN12 participate in male infertility, especially Sertoli cell-only syndrome (SCOS). To investigate whether SEPTIN12 gene defects are associated with azoospermia caused by SCOS, mutational analysis was performed in 100 Japanese patients by direct sequencing of coding regions. Statistical analysis was performed in patients with SCOS and in 140 healthy control men. No mutations were found in SEPTIN12 ; however, 8 coding single-nucleotide polymorphisms (SNP1-SNP8) could be detected in the patients with SCOS. The genotype and allele frequencies in SNP3, SNP4, and SNP6 were notably higher in the SCOS group than in the control group (P < .001). These results suggest that SEPTIN12 might play a critical role in human spermatogenesis.
Acta Obstetricia et Gynecologica Scandinavica | 2013
Kazuo Sengoku; Toshinobu Miyamoto; Michiharu Horikawa; Hideto Katayama; Kunihiko Nishiwaki; Yasuhito Kato; Yasuyuki Kawanishi; Yasuaki Saijo
To identify epidemiologic risk factors and investigate whether the characteristics of removed ovarian tissue during surgery influence the recurrence of endometriomas.
Journal of Human Genetics | 2012
Yukiko Kondo; Hirotomo Saitsu; Toshinobu Miyamoto; Kiyomi Nishiyama; Yoshinori Tsurusaki; Hiroshi Doi; Noriko Miyake; Na-Kyung Ryoo; Jeong Hun Kim; Young Suk Yu; Naomichi Matsumoto
Oculofaciocardiodental syndrome (OFCD) is an X-linked dominant disorder associated with male lethality, presenting with congenital cataract, dysmorphic face, dental abnormalities and septal heart defects. Mutations in BCOR (encoding BCL-6-interacting corepressor) cause OFCD. Here, we report on a Korean family with common features of OFCD including bilateral 2nd–3rd toe syndactyly and septal heart defects in three affected females (mother and two daughters). Through the mutation screening and copy number analysis using genomic microarray, we identified a novel heterozygous mutation, c.888delG, in the BCOR gene and two interstitial microduplications at Xp22.2–22.13 and Xp21.3 in all the three affected females. The BCOR mutation may lead to a premature stop codon (p.N297IfsX80). The duplication at Xp22.2–22.13 involved the NHS gene causative for Nance–Horan syndrome, which is an X-linked disorder showing similar clinical features with OFCD in affected males, and in carrier females with milder presentation. Considering the presence of bilateral 2nd–3rd toe syndactyly and septal heart defects, which is unique to OFCD, the mutation in BCOR is likely to be the major determinant for the phenotypes in this family.
Journal of Assisted Reproduction and Genetics | 2011
Toshinobu Miyamoto; Akira Tsujimura; Yasushi Miyagawa; Eitetsu Koh; Mikio Namiki; Michiharu Horikawa; Yasuaki Saijo; Kazuo Sengoku
PurposeTo investigate the association between the UBR2 gene and the risk of azoospermia caused by meiotic arrest.MethodsMutational analysis of the UBR2 gene was performed using DNA from 30 patients with azoospermia by meiotic arrest to 80 normal controls.ResultsThe genotypic and allelic frequencies of c.1,066A>T variant were significantly higher in patient than control groups (pu2009<u20090.001).ConclusionThe c.1,066A>T variant in the UBR2 gene is associated with increased susceptibility to azoospermia caused by meiotic arrest.
Journal of Andrology | 2016
Toshinobu Miyamoto; Y. Bando; Eitetsue Koh; Akira Tsujimura; Yasushi Miyagawa; Masashi Iijima; Mikio Namiki; M. Shiina; K. Ogata; Naomichi Matsumoto; Kazuo Sengoku
About 15% of couples wishing to have children are infertile; approximately half these cases involve a male factor. Polo‐like kinase 4 (PLK‐4) is a member of the polo protein family and a key regulator of centriole duplication. Male mice with a point mutation in the Plk4 gene show azoospermia associated with germ cell loss. Mutational analysis of 81 patients with azoospermia and Sertoli cell‐only syndrome (SCOS) identified one man with a heterozygous 13‐bp deletion in the Ser/Thr kinase domain of PLK4. Division of centrioles occurred in wild‐type PLK4‐transfected cells, but was hampered in PLK‐4‐mutant transfectants, which also showed abnormal nuclei. Thus, this PLK4 mutation might be a cause of human SCOS and nonobstructive azoospermia.
Reproductive Medicine and Biology | 2017
Toshinobu Miyamoto; Gaku Minase; Takeshi Shin; Hiroto Ueda; Hiroshi Okada; Kazuo Sengoku
Infertility affects about 15% of couples who wish to have children and half of these cases are associated with male factors. Genetic causes of azoospermia include chromosomal abnormalities, Y chromosome microdeletions, and specific mutations/deletions of several Y chromosome genes. Many researchers have analyzed genes in the AZF region on the Y chromosome; however, in 2003 the SYCP3 gene on chromosome 12 (12q23) was identified as causing azoospermia by meiotic arrest through a point mutation.
Asian Journal of Andrology | 2012
Toshinobu Miyamoto; Akira Tsujimura; Yasushi Miyagawa; Eitetsu Koh; Mikio Namiki; Michiharu Horikawa; Yasuaki Saijo; Kazuo Sengoku
Genetic mechanisms are implicated as a cause of some male infertility, yet are poorly understood. Meiosis is unique to germ cells and essential for reproduction. The synaptonemal complex is a critical component for chromosome pairing, segregation and recombination. Hormad1 is essential for mammalian gametogenesis as knockout male mice are infertile. Hormad1-deficient testes exhibit meiotic arrest in the early pachytene stage and synaptonemal complexes cannot be visualized. To analyze the hypothesis that the human HORMAD1 gene defects are associated with human azoospermia caused by meiotic arrest, mutational analysis was performed in all coding regions by direct sequence analysis of 30 Japanese men diagnosed with azoospermia resulting from meiotic arrest. By the sequence analysis, three polymorphism sites, Single Nucleotide Polymorphism 1 (c. 163A>G), SNP2 (c. 501T>G) and SNP3 (c. 918C>T), were found in exons 3, 8 and 10. The 30 patients with azoospermia and 80 normal pregnancy-proven, fertile men were analyzed for HORMAD1 polymorphisms. Both SNP1 and SNP2 were associated with human azoospermia caused by complete early meiotic arrest (P<0.05). We suggest that the HORMAD1 has an essential meiotic function in human spermatogenesis.