Toshiro Furukawa

Selection of high brassylic acid producing strains of Torulopsis candida by single-cell cloning and by mutation

Abstract Single-cell colonies of Torulopsis candida NC-3-58 varied in their productivity of brassylic acid (1,11-undecanedicarboxylic acid). Several strains such as M-4-2 and M-5-1 with higher yields of brassylic acid have been isolated by repeated single-cell cloning. Mutagenesis of M-5-1 with UV irradiation gave strains such as Bu-1, and Bu-1-2 that produced much brassylic acid. Brassylic acid and n -tridecane degradation among the parent and mutant strains was compared.

The Formation of Higher Alcohols in the Fermentation of Amino Acids by Yeast: The Determination of Alcohols with Gas Chromatography

We have found that some straight-chained α-amino acids are converted by yeast to the alcohols with correspondingly longer carbon chains in the alcoholic fermentation contrary to F. Ehrlich’s scheme, i.e., isobutyl alcohol from alanine and active amyl alcohol from α-amino-n-butyric acid or threonine.In this report, we confirmed this fact in the alcoholic fermentation of many aliphatic amino acids by 2 yeast strains using gas chromatography. Moreover, n-propyl alcohol was proved to come from α-amino-n-butyric acid or threonine. Small quantities of n-propyl, isobutyl, active amyl and isoamyl alcohols were found in all the fermented solutions. There was some difference in the composition of higher alcohols of the alcoholic solutions fermented by different yeasts.

Studies on the Utilization of Hydrocarbons by Microorganisms: Part XVI. Detection of Metabolic Intermediates of Xylene and Pseudocumene Part XVII. Metabolism of p-Xylene and Related Compounds Part XVIII. Fermentative Production of Fumaric Acid by Candida hydrocarbofumarica n. sp.

In the course of investigation of the metabolism of p-xylene, we detected p-cresol, 4-methylcatechol and p-hydroxybenzoic acid from culture broth.Pseudomonas aeruginosa S668B2 was found to utilize benzoic acid, p-toluic acid, m-toluic acid and 3,4-dimethyl benzoic acid as a carbon source.The strain S668B2 was shown to produce p-cresol from p-toluic acid, 3-methyl salicylic acid from m-toluic acid and 3,4-dimethyl phenol from 3,4-dimethyl benzoic acid.The strain S668B2 also produced p-hydroxy benzoic acid from p-cresol.Pathways of the metabolism of m-xylene and pseudocumene were speculated.