Totok K. Waluyo

Enzymatic Saccharification of Soda Pulp from Sago Starch Waste Using Sago Lignin-Based Amphipathic Derivatives

Abstract The aim of this research is to develop an enzymatic saccharification process of sago starch waste, with a small charge of cellulase. The waste contained a significant amount of residual starch, which was recovered as glucose by mild acid hydrolysis. The starch-free residue was subjected to soda-anthraquinone pulping to yield soda pulp and soda lignin. The lignin was converted to amphipathic lignin derivatives by the reaction with epoxylated polyethylene glycol analogues. The pulp was hydrolyzed with cellulase (Genencor GC220), with the amphipathic derivatives, to yield glucose. The lignin derivative-assisted, enzymatic saccharification was repeatedly conducted by reusing cellulase recovered by ultrafiltration from saccharification media. Saccharification efficiency with the derivatives was maintained at a high level even after the fourth run of saccharification, while the efficiency was remarkably decreased by repeated use of cellulase without additive. Thus, the amphipathic sago lignin derivatives enabled repeated use of cellulase for saccharification of sago starch waste.

APLIKASI ARANG KOMPOS BIOAKTIF PADA BUDIDAYA NILAM (Pogostemon cablin Benth) TERHADAP KUALITAS PRODUK MINYAK NILAM

Nilam ( Pogostemon cablin Benth) merupakan salah satu tanaman penghasil minyak atsiri yang penting karena memiliki potensi strategis di pasar dunia dimana minyak tersebut berfaedah sebagai bahan pengikat aroma wangi pada parfum, kosmetika dan bahan aromaterapi. Terkait dengan uraian tersebut tulisan ini menyajikan tentang pengaruh pemberian arang kompos bioaktif (arkoba) terhadap kualitas minyak atsiri hasil penyulingan daun nilam antara lain rendemen dan kadar patchouli alkohol. Hasil penelitian menunjukkan bahwa penambahan arkoba pada budidaya nilam memberikan pengaruh sangat baik terhadap rendemen minyak nilam, mencapai 3-4,5%, dengan rata-rata 4 %, sedangkan rendemen minyak nilam yang ditanam tanpa menggunakan arkoba hanya berkisar 2-2,3% (rata-rata 2%). Lebih lanjut kadar patchouli alkohol minyak nilam dengan penggunaan arkoba yaitu 40,01%, sedangkan yang tanpa diberi arkoba hanya 32,26%.