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Dive into the research topics where Trinh Pham is active.

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Featured researches published by Trinh Pham.


Nature Nanotechnology | 2013

Sensitive capture of circulating tumour cells by functionalized graphene oxide nanosheets

Hyeun Joong Yoon; Tae Hyun Kim; Zhuo Zhang; Ebrahim Azizi; Trinh Pham; Costanza Paoletti; Jules Lin; Nithya Ramnath; Max S. Wicha; Daniel F. Hayes; Diane M. Simeone; Sunitha Nagrath

The spread of cancer throughout the body is driven by circulating tumour cells (CTCs)1. These cells detach from the primary tumour and move from the blood stream to a new site of subsequent tumour growth. They also carry information about the primary tumour and have the potential to be valuable biomarkers for disease diagnosis and progression, and for the molecular characterization of certain biological properties of the tumour. However, the limited sensitivity and specificity of current methods to measure and study these cells in patient blood samples prevent the realization of their full clinical potential. The use of microfluidic devices is a promising method for isolating CTCs2, 3; however, the devices are reliant on three-dimensional structures, which limit further characterization and expansion of cells on the chip. Here we demonstrate an effective approach to isolate CTCs from blood samples of pancreatic, breast and lung cancer patients, by using functionalised graphene oxide nanosheets on a patterned gold surface. CTCs were captured with high sensitivity at low concentration of target cells (73% ± 32.4 at 3–5 cells/mL blood).


Annals of Surgery | 2000

Disruption of TGFβ Signaling Pathways in Human Pancreatic Cancer Cells

Diane M. Simeone; Trinh Pham; Craig D. Logsdon

ObjectiveTo investigate whether transforming growth factor beta (TGFβ) signaling is disrupted in human pancreatic cancer cells, and to study the role of TGFβ receptors and Smad genes.Summary Background DataTGFβ is a known inhibitor of pancreatic growth. Disruption of the TGFβ signaling pathway may p


Journal of Microbiological Methods | 2017

Diagnostic microarray for 14 water and foodborne pathogens using a flatbed scanner

Vidya Srinivasan; Robert D. Stedtfeld; Dieter M. Tourlousse; Samuel W. Baushke; Yu Xin; Sarah M. Miller; Trinh Pham; Jean Marie Rouillard; Erdogan Gulari; James M. Tiedje; Syed A. Hashsham

Parallel detection approaches are of interest to many researchers interested in identifying multiple water and foodborne pathogens simultaneously. Availability and cost-effectiveness are two key factors determining the usefulness of such approaches for laboratories with limited resources. In this study, we developed and validated a high-density microarray for simultaneous screening of 14 bacterial pathogens using an approach that employs gold labeling with silver enhancement (GLS) protocol. In total, 8887 probes (50-mer) were designed using an in-house database of virulence and marker genes (VMGs), and synthesized in quadruplicate on glass slides using an in-situ synthesis technology. Target VMG amplicons were obtained using multiplex polymerase chain reaction (PCR), labeled with biotin, and hybridized to the microarray. The signals generated after gold deposition and silver enhancement, were quantified using a flatbed scanner having 2-μm resolution. Data analysis indicated that reliable presence/absence calls could be made, if: i) over four probes were used per gene, ii) the signal-to-noise ratio (SNR) cutoff was greater than or equal to two, and iii) the positive fraction (PF), i.e., number of probes with SNR≥2 for a given VMG was greater than 0.75. Hybridization of the array with blind samples resulted in 100% correct calls, and no false positive. Because amplicons were obtained by multiplex PCR, sensitivity of this method is similar to PCR. This assay is an inexpensive and reliable technique for high throughput screening of multiple pathogens.


Nature Genetics | 1995

Somatic mutations in the BRCA1 gene in sporadic ovarian tumours

Sofia D. Merajver; Trinh Pham; Rosemarie F. Caduff; Martha Chen; Ellen L. Poy; Kathleen A. Cooney; Barbara L. Weber; Francis S. Collins; Carolyn Johnston; Thomas S. Frank


Angewandte Chemie | 2007

Signal‐Amplifying Conjugated Polymer–DNA Hybrid Chips

Kangwon Lee; Jean Marie Rouillard; Trinh Pham; Erdogan Gulari; Jinsang Kim


Clinical Cancer Research | 1995

Germline BRCA1 mutations and loss of the wild-type allele in tumors from families with early onset breast and ovarian cancer.

Sofia D. Merajver; Thomas S. Frank; Junzhe Xu; Trinh Pham; Kathleen A. Calzone; Pamela E. Bennett-Baker; Jeffrey S. Chamberlain; Jeff Boyd; Judy Garber; Francis S. Collins; Barbara L. Weber


American Journal of Physiology-cell Physiology | 2001

Smad4 mediates activation of mitogen-activated protein kinases by TGF-β in pancreatic acinar cells

Diane M. Simeone; Lizhi Zhang; Kathleen Graziano; Barbara Nicke; Trinh Pham; Claus Schaefer; Craig D. Logsdon


American Journal of Physiology-gastrointestinal and Liver Physiology | 2001

Adenovirus-mediated gene transfer of dominant-negative Smad4 blocks TGF-β signaling in pancreatic acinar cells

Lizhi Zhang; Kathleen Graziano; Trinh Pham; Craig D. Logsdon; Diane M. Simeone


Archive | 2007

Signal-Amplifying Conjugated Polymer–DNA Hybrid Chips We appreciate financial support from the National Science Foundation (BES 0428010) and the equipment grant from the University of Michigan College of Engineering. J.K. and K.L. also acknowledge the Ilju Foundation for the Ilju scholarship.

Kangwon Lee; Jean-Marie Rouillard; Trinh Pham; Erdogan Gulari; Jinsang Kim


Nature Nanotechnology | 2013

Erratum: Sensitive capture of circulating tumour cells by functionalized graphene oxide nanosheets (Nature Nanotechnology (2013) 8 (735-741))

Hyeun Joong Yoon; Tae Hyun Kim; Zhuo Zhang; Ebrahim Azizi; Trinh Pham; Costanza Paoletti; Jules Lin; Nithya Ramnath; Max S. Wicha; Daniel F. Hayes; Diane M. Simeone; Sunitha Nagrath

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Craig D. Logsdon

University of Texas MD Anderson Cancer Center

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Francis S. Collins

National Institutes of Health

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