Trinh Pham
University of Michigan
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Publication
Featured researches published by Trinh Pham.
Nature Nanotechnology | 2013
Hyeun Joong Yoon; Tae Hyun Kim; Zhuo Zhang; Ebrahim Azizi; Trinh Pham; Costanza Paoletti; Jules Lin; Nithya Ramnath; Max S. Wicha; Daniel F. Hayes; Diane M. Simeone; Sunitha Nagrath
The spread of cancer throughout the body is driven by circulating tumour cells (CTCs)1. These cells detach from the primary tumour and move from the blood stream to a new site of subsequent tumour growth. They also carry information about the primary tumour and have the potential to be valuable biomarkers for disease diagnosis and progression, and for the molecular characterization of certain biological properties of the tumour. However, the limited sensitivity and specificity of current methods to measure and study these cells in patient blood samples prevent the realization of their full clinical potential. The use of microfluidic devices is a promising method for isolating CTCs2, 3; however, the devices are reliant on three-dimensional structures, which limit further characterization and expansion of cells on the chip. Here we demonstrate an effective approach to isolate CTCs from blood samples of pancreatic, breast and lung cancer patients, by using functionalised graphene oxide nanosheets on a patterned gold surface. CTCs were captured with high sensitivity at low concentration of target cells (73% ± 32.4 at 3–5 cells/mL blood).
Annals of Surgery | 2000
Diane M. Simeone; Trinh Pham; Craig D. Logsdon
ObjectiveTo investigate whether transforming growth factor beta (TGFβ) signaling is disrupted in human pancreatic cancer cells, and to study the role of TGFβ receptors and Smad genes.Summary Background DataTGFβ is a known inhibitor of pancreatic growth. Disruption of the TGFβ signaling pathway may p
Journal of Microbiological Methods | 2017
Vidya Srinivasan; Robert D. Stedtfeld; Dieter M. Tourlousse; Samuel W. Baushke; Yu Xin; Sarah M. Miller; Trinh Pham; Jean Marie Rouillard; Erdogan Gulari; James M. Tiedje; Syed A. Hashsham
Parallel detection approaches are of interest to many researchers interested in identifying multiple water and foodborne pathogens simultaneously. Availability and cost-effectiveness are two key factors determining the usefulness of such approaches for laboratories with limited resources. In this study, we developed and validated a high-density microarray for simultaneous screening of 14 bacterial pathogens using an approach that employs gold labeling with silver enhancement (GLS) protocol. In total, 8887 probes (50-mer) were designed using an in-house database of virulence and marker genes (VMGs), and synthesized in quadruplicate on glass slides using an in-situ synthesis technology. Target VMG amplicons were obtained using multiplex polymerase chain reaction (PCR), labeled with biotin, and hybridized to the microarray. The signals generated after gold deposition and silver enhancement, were quantified using a flatbed scanner having 2-μm resolution. Data analysis indicated that reliable presence/absence calls could be made, if: i) over four probes were used per gene, ii) the signal-to-noise ratio (SNR) cutoff was greater than or equal to two, and iii) the positive fraction (PF), i.e., number of probes with SNR≥2 for a given VMG was greater than 0.75. Hybridization of the array with blind samples resulted in 100% correct calls, and no false positive. Because amplicons were obtained by multiplex PCR, sensitivity of this method is similar to PCR. This assay is an inexpensive and reliable technique for high throughput screening of multiple pathogens.
Nature Genetics | 1995
Sofia D. Merajver; Trinh Pham; Rosemarie F. Caduff; Martha Chen; Ellen L. Poy; Kathleen A. Cooney; Barbara L. Weber; Francis S. Collins; Carolyn Johnston; Thomas S. Frank
Angewandte Chemie | 2007
Kangwon Lee; Jean Marie Rouillard; Trinh Pham; Erdogan Gulari; Jinsang Kim
Clinical Cancer Research | 1995
Sofia D. Merajver; Thomas S. Frank; Junzhe Xu; Trinh Pham; Kathleen A. Calzone; Pamela E. Bennett-Baker; Jeffrey S. Chamberlain; Jeff Boyd; Judy Garber; Francis S. Collins; Barbara L. Weber
American Journal of Physiology-cell Physiology | 2001
Diane M. Simeone; Lizhi Zhang; Kathleen Graziano; Barbara Nicke; Trinh Pham; Claus Schaefer; Craig D. Logsdon
American Journal of Physiology-gastrointestinal and Liver Physiology | 2001
Lizhi Zhang; Kathleen Graziano; Trinh Pham; Craig D. Logsdon; Diane M. Simeone
Archive | 2007
Kangwon Lee; Jean-Marie Rouillard; Trinh Pham; Erdogan Gulari; Jinsang Kim
Nature Nanotechnology | 2013
Hyeun Joong Yoon; Tae Hyun Kim; Zhuo Zhang; Ebrahim Azizi; Trinh Pham; Costanza Paoletti; Jules Lin; Nithya Ramnath; Max S. Wicha; Daniel F. Hayes; Diane M. Simeone; Sunitha Nagrath