Tsuneo Mizushima

A comparative histochemical evaluation of various dehydrogenases in the oral squamous epithelium

SummaryA histochemical observation was made of various dehydrogenase activities in oral squamous epithelia. The localization of dehydrogenases showed a relatively similiarity except for the intensity of the dehydrogenase activity. Succinic dehydrogenase activity was generally confined to the basal cell layer and adjacent cell layers; superficial layers did not show any enzymatic activity. Lactic, and malic dehydrogenase activities were localized in the basal cells to st. granulosum, and the activity of lactic dehydrogenase was the highest. α-Glycerophosphate, glutamic, glucose-6-phosphate and TPN-isocitric dehydrogenase activities were observed in all the epithelial cells with the exception for the hornified layer, and they were found generally low. β-Hydroxybutyric dehydrogenase was low and contained in both of st. germinativum and st. granulosum, the keratohyalin in st. granulosum being occasionally found reactive to this enzymatic activity.In connective tissue cells and collagen bundels, activities of lactic, and malic dehydrogenase were intense, while other dehydrogenases were low or trace amount.In the oral squamous epithelium under normal conditions, the dehydrogenase localization concerning the glucose metabolism and TCA cycle member and other close pathways was not similar. Nor were their activities found likewise. Those findings lead to a conclusion that the epithelial cells of the same layer many show a selective metabolic activity.

Histochemical studies of dehydrogenases in the developing teeth

SummaryHistochemical observations were made of oxidative and reducing enzymes in relation to several metabolic pathways in the developing tooth. The localization and activity of dehydrogenases and oxidases could be divided roughly into two types.Type one refers to enzymatic activities of lactic, succinic, malic and glucose-6-phosphate dehydrogenase and aconitase and cytochrome oxidase, which were low in undifferentiated dental epithelium and increased in proportion to cell differentiation. Comparatively high enzymatic activities were reflected in high cell function.Type two did not increase in the enzymatic activity during differentiation. Glyceraldehyde-3-phosphate, TPN isocitric, 6-phosphogluconate, glutamic, α-glycerophosphate and β-hydroxybutyric dehydrogenase and monoamine oxidase belong to this type.

Histochemical comparison of oxidative enzymes in adrenal glands of mammals

SummarySuccinic dehydrogenase, five DPN-linked dehydrogenases-lactic dehydrogenase, malic dehydrogenase, glutamic dehydrogenase, α-glycerophosphate dehydrogenase, β-hydroxybutyric dehydrogenase, two TPN linked dehydrogenases — glucose-6-phosphate dehydrogenase, isocitric dehydrogenase and 3β-ol steroid dehydrogenase were studied in mouse, rat, guinea pig, rabbit, dog, cat, cow, monkey and human adrenal glands. Histochemical studies were made of a characteristic distribution of different level of enzyme activity. In mammals adrenal glands, glucose-6-phosphate dehydrogenase showed the highest activity and its localization was divided into the following two groups: 1) High enzymatic activity was demonstrated in the zona fasciculata and reticularis of the rat, guinea pig, rabbit, cat and 2) high enzymatic activity was demonstrated in the zona glomerulosa and reticularis of the dog, cow and monkey. A precise relationship between the localization and endocrinological function remains abscure.

HISTOCHEMICAL OBSERVATIONS OF SUCCINIC, MALIC, AND LACTIC DEHYDROGENASE IN ORAL TUMORS.

Abstract A histochemical demonstration of succinic, malic, and lactic dehydrogenase in fifty-two cases of oral tumor has been carried out. Histochemical localization of the three dehydrogenases was almost the same except for their activity, lactic dehydrogenase being the highest and succinic dehydrogenase rather low. Succinic dehydrogenase activity was lower in the malignant neoplastic epithelium than in the homologous epithelium. Pearl formation areas of central foci in epidermoid cancer and the acanthoma type of ameloblastoma were devoid of activities by any dehydrogenase. Succinic dehydrogenase activity of the connective tissue stromas was negligible, but proliferating collagen bundles showed considerable activity. In general, the tumor stroma showed a marked DPN-linked dehydrogenase activity.