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Featured researches published by Tsutomu Masuda.


Biochimica et Biophysica Acta | 1992

Purification and characterization of luciferases from fireflies, Luciola cruciata and Luciola lateralis

Naoki Kajiyama; Tsutomu Masuda; Hiroki Tatsumi; Eiichi Nakano

Luciferases of Luciola cruciata and Luciola lateralis, LcL and LlL, were purified to homogeneity by ammonium sulfate precipitation, gel-filtration column chromatography, and hydroxyapatite HPLC. The molecular masses of the enzymes determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were both 62 kDa, almost identical to that of Photinus pyralis (PpL). LcL was found to be similar to PpL in thermal stability, pH stability, and the wavelength of maximum light intensity. LlL was superior to LcL and PpL in thermal and pH stability, and the reaction catalyzed by LlL emits green light with a peak intensity at 552 nm, which is 10 nm shorter in wavelength than those of PpL and LcL.


Archive | 1988

Luciferase gene and novel recombinant DNA as well as a method of producing luciferase

Tsutomu Masuda; Hiroki Tatsumi; Eiichi Nakano


Journal of Bioluminescence and Chemiluminescence | 1989

Luciferase cDNA from Japanese firefly, Luciola cruciata: Cloning, structure and expression in Escherichia coli

Hiroki Tatsumi; Tsutomu Masuda; Naoki Kajiyama; Eiichi Nakano


Archive | 1991

Purified luciferase from luciola cruciata

Naoki Kajiyama; Tsutomu Masuda; Hiroki Tatsumi; Eiichi Nakano


Journal of Biochemistry | 1986

Synthesis and characterization of human prorenin in Escherichia coli.

Takashi Imai; Takeshi Cho; Hiroyuki Takamatsu; Hitoshi Hori; Michiya Saito; Tsutomu Masuda; Shigehisa Hirose; Kazuo Murakami


Biomedical Research-tokyo | 1982

MOLECULAR CLONING OF DNA COMPLEMENTARY TO MOUSE SUBMANDIBULAR GLAND RENIN mRNA

Tsutomu Masuda; Takashi Imai; Takao Fukushi; Masayuki Sudoh; Shigehisa Hirose; Kazuo Murakami


Archive | 1991

Purified luciferase from luciola lateralis

Naoki Kajiyama; Tsutomu Masuda; Hiroki Tatsumi; Eiichi Nakano


Agricultural and biological chemistry | 1988

Synthesis of Enzymatically Active Firefly Luciferase in Yeast

Hiroki Tatsumi; Tsutomu Masuda; Eiichi Nakano


Journal of Biochemistry | 1987

Affinity purification and characterization of serine hydroxymethyltransferases from rat liver

Tsutomu Masuda; Masako Sakamoto; Izumi Nishizaki; Hideyuki Hayashi; Maya Yamamoto; Hiroshi Wada


Biomedical Research-tokyo | 1982

ISOLATION AND TRANSLATION OF RENIN mRNA FROM THE MOUSE SUBMANDIBULAR GLAND

Takao Fukushi; Tsutomu Masuda; Takashi Imai; Masayuki Sudoh; Sadao Kimura; Shigehisa Hirose; Kazuo Murakami

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Shigehisa Hirose

Tokyo Institute of Technology

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