Tu Pengfei

Triterpenoid saponins from Ilex mamillata C.Y. Wu ex C.J. Tseng

Two new triterpenoid saponins, ilemaminosides A and B (1 and 2) along with six known saponins (3–8) were isolated from 70% ethanolic extract of the leaves of Ilex mamillata C.Y. Wu ex C.J. Tseng. The new saponins were characterised as 3-O-α-L-arabinopyranosyl-ilexgenin B (1) and 3-O-β-D-glucopyranosyl-(1 → 3)-α-L-arabinopyranosyl-ilexgenin B (2). The structures of compounds 1 and 2 were elucidated on the basis of the chemical and spectroscopic methods, and the structures of known compounds were identified by comparison of their spectroscopic data with those reported in the literature. The compounds showed inhibitory activities in anti-inflammatory assay in vitro with IC50 values in the range 25.37–38.33 µg mL−1.

Discrimination of Alismatis Rhizoma based on chromatographic fingerprint, multiple components quantification, and pattern recognition analysis

ABSTRACTA simple, sensible, and reliable HPLC–DAD method was first developed for fingerprint analysis of Alismatis Rhizoma, and then applied to analyze 85 samples from three main cultivated areas. In all, 40 common fingerprint peaks were designated, and six of which were definitely identified. Then, the combinatory analysis using similarity evaluation, principal component analysis, and orthogonal partial least square discriminant analysis revealed clear chemical consistency between samples from Fujian and Jiangxi provinces and substantial differences between those from Fujian/Jiangxi and Sichuan provinces. Furthermore, six components were dug out as potential chemical markers for distinguishing Alismatis Rhizoma from different areas, among which five were qualified for quantitative analysis. In conclusion, the combination of chemical fingerprint, multiple components quantification, and pattern recognition analysis was rather powerful and useful in discriminating Alismatis Rhizoma from different regions, w...ABSTRACT A simple, sensible, and reliable HPLC–DAD method was first developed for fingerprint analysis of Alismatis Rhizoma, and then applied to analyze 85 samples from three main cultivated areas. In all, 40 common fingerprint peaks were designated, and six of which were definitely identified. Then, the combinatory analysis using similarity evaluation, principal component analysis, and orthogonal partial least square discriminant analysis revealed clear chemical consistency between samples from Fujian and Jiangxi provinces and substantial differences between those from Fujian/Jiangxi and Sichuan provinces. Furthermore, six components were dug out as potential chemical markers for distinguishing Alismatis Rhizoma from different areas, among which five were qualified for quantitative analysis. In conclusion, the combination of chemical fingerprint, multiple components quantification, and pattern recognition analysis was rather powerful and useful in discriminating Alismatis Rhizoma from different regions, which was a benefit for quality control. GRAPHICAL ABSTRACT

在线加压溶剂微提取-湍流色谱-高效液相色谱法同时测定管花肉苁蓉中3种苯乙醇苷

建立了在线加压溶剂微提取-湍流色谱-高效液相色谱(online PLME-TFC-HPLC)法,并将其应用于管花肉苁蓉中松果菊苷、毛蕊花糖苷和异毛蕊花糖苷3种苯乙醇苷类成分含量的同时测定。微量样品粉末(0.5 mg)置于空预柱芯中并用正相硅胶填充,得到提取池后装入预柱套(Security Guard TM )。将预柱套置于70℃柱温箱中,将一根长聚醚醚酮(PEEK)管线(1000 mm×0.13 mm)连于预柱套末端,采用0.1%(v/v)甲酸水为提取溶剂,以2.5 mL/min的速度流经PEEK管线,产生高压,实现管花肉苁蓉的在线加压溶剂微提取,通过TurboFlow cyclone色谱柱在线净化和富集。引入两个电子六通阀,将整个分析过程分为提取阶段和洗脱阶段,并在洗脱阶段将TurboFlow cyclone色谱柱中的分析物反冲至Capcell PAK C 18 AQ分析柱上,以0.1%(v/v)甲酸水-乙腈为流动相进行梯度洗脱,以340 nm为检测波长同时定量分析松果菊苷、毛蕊花糖苷和异毛蕊花糖苷3种苯乙醇苷类成分。结果表明,3种苯乙醇苷类在1~200 mg/L范围内线性良好,相关系数 r 均大于0.999,定量限分别为0.50 mg/L(松果菊苷)、0.25 mg/L(毛蕊花糖苷)和0.38 mg/L(异毛蕊花糖苷),加标回收率为83.13%~114.00%,相对标准偏差为1.89%~13.34%。该方法简便、快速、可靠,不仅节约了药材和溶剂的使用量,而且极大地简化了前处理方法,省时省力,同时显著降低了化学成分在提取过程中降解的几率,适用于管花肉苁蓉中苯乙醇苷类化合物的含量测定。