Tu Yunjie

AFLP fingerprinting analysis of genetic polymorphism of 12 indigenous chicken breeds

A total of six amplified fragment length polymorphism (AFLP) primer combinations were used to detect genetic variation of pooled DNA in a sample of 12 chicken breeds indigenous to China, and AFLP DNA fingerprinting of each chicken breed was constructed. Polymorphic bands, specific bands and genetic similarity coefficients of 12 chicken breeds were calculated from AFLP data. A total of 279 polymorphic bands were generated by the six primer combinations, giving, on average, 46.5 polymorphic markers detected per primer combination. Nine specific bands were produced in the pooled DNA of Jiuyuan black and Dongxiang black chickens. However, one specific band was produced in the pooled DNA of Wenchang and Xingyi bantam chickens. An unweighted-pair-group method using average linkages (UPGMA) cluster analysis revealed that the 12 chicken breeds could be divided into three groups. Genetic similarity coefficients and the UPGMA tree of the 12 chicken breeds were consistent with their breeding history as well as their geographical distribution. Based on AFLP DNA fingerprinting, genetic diversity, genetic relationship and identification of chicken breeds can be analysed.

Effect of single and pyramiding genotypes of GH and POU1F1 on egg production of Baier chicken.

The chicken GH 和 POU1F1 gene was selected as candidate genes,PCR-SSCP method was used to identify the polymorphism sites in the exon4 region of GH gene and in the exon3 region of POU1F1 gene. Association between the founded polymorphism sites as well as pyramiding genotypes and egg production traits were tested by least square analysis. The results showed the founded polymorphism sites (C2338G\C2341T) of GH gene exon4 had a significant correlation with the egg number at 72 weeks(P0.05). Additive effects of genotype AA for GH genes were 3.80. The founded polymorphism sites(A5331T) POU1F1 gene exon3 had a significant correlation with egg production at 72 weeks age. Additive effects of genotype CC for POU1F1 genes were 3.57. The pyramiding genotype AACC had higher 72 weeks of age egg production compared to that of other pyramiding genotypes(P0.05). Its interactive effect was not significant (P0.05). Eee production of generation F1 was much the same as that of their motherhood.