Turan Tank

Genotype Differencies in Direct Plant Regeneration from Stem Explants of Populus tremula in Turkey

Plant regeneration from 9 clones ofPopulus tremula representing diverse genotypes originated from Turkey, has been studied in different media, such as Murashige and Skoog’s Medium (MS), Aspen Culture Medium (ACM) and Woody Plant Medium (WPM) with appropriate supplements of growth regulators. Regeneration efficiency on WPM containing 1 mg/l zeatin using stem explantsin vitro, was higher than on MS and ACM. In this system, the time required for shoot regeneration was 2 weeks, which was shorter by two weeks than that of previous reports. A strong genotype dependence was observed among the tested nine clones. The regeneration system, we describe here, did not involve the callus phase and thus was less prone to somaclonal variation. When adventitious microshoots were cultured on WPM supplemented with 0.5 mg/l or 2 mg/l indole-3-butyric acid (IBA), root formation was obtained after 1 week of culture. Adaptation of regenerated plantletsin vivo was 100%. This system for micropropagation ofPopulus tremula is expected to be suitable for studies on transformation of genes involved in lignin biosynthesis via modification of lignin content.

agrobacterium-Mediated Transformation of Peroxidase Gene to Tissue Culture of populus Tremula

Tissue culture of Populus tremula Dursunbey 2 clone from stem expiants was established. peroxidase gene isolated from Stylosanthes humilis (Shpx6), in antisense orientation was transferred to poplar via Agrobacterium tumefaciens. The effects of Shpx6 gene leads to modification of total peroxidase activity and total lignin amount. A reduction of total peroxidase activity for 50% and total lignin amont for 20% was observed in transgenic plants compared with control plants. The results obtained here, could be useful for utilization of poplar in paper and pulp industry.

Regeneration and Transformation of populus Tremula with Antisense pOMT8 from stylosanthes Humilis

Populus tremula was transformed using Agrobacterium tumefaciens with antisens O-metyltransferase gene from Stylosanthes humilis (pOMT8), which is showing 83% homology with COMT gene from poplar. In four out of ten transgenic plants OMT activity has been downregulated for 30–39%, however peroxidase activity was 100% higher compared with controls. While the lignin amount in transgenic plants was not changed, lignin composition were modified. Results indicate that it is possible to downregulate endogenous OMT activity and modified lignin composition through the introduction of heterologous consract from Stylosanthes humilis in Populus tremula.

Almanya selüloz ve kağıt kimyagerleri ve mühendisleri derneğince tertiplenen 1969 yılı uluslararası kongreden izlenimler

Almanya seluloz ve kagit kimyagerleri ve muhendisleri dernegince tertiplenen 1969 yili uluslararasi kongreden izlenimler