Tutik Nurhidayati

Reverse Transcription-PCR analysis of geranylgeranyl diphosphate synthase (JcGGPPS) in Jatropha curcas L. and in silico analysis of Casbene Synthase (JcCS) among Euphorbiaceae

Recently, Euphorbiaceae have attracted much attention for their potential uses in biodiesel production. Their seeds have been well known as the principal resource for oil production. The seed-derived oil content ranged from 28 % to 39 % by dry weight for all Euphorbiaceae species. Meanwhile, their seed also possesses relatively high protein content, ranged from 22 % to 35 %, making them a new alternative for livestock animal feed resources. Nevertheless, the development of this new animal feed resource is limited to the toxicity of the seeds. Its toxicity is mainly caused by the presence of anti-nutrient agents and toxins, such as curcin, trypsin inhibitor and phorbol ester (PE). The later is known to be tetracyclic diterpenes, which represents the primary toxic substances in one of the Euphorbiaceae plants, Jatropha curcas L. To date, little is known about the biosynthesis of these toxic substances. This research aims to analyze the expression of JcGGPPS gene encoding the geranylgeranyl diphosphate synth...

Particular variety of tobacco Nicotiana tabacum) exhibits distinct morphological and physiological responses against periodic waterlogging stress

Waterlogging Stress influences crop productivity, especially high commercial crop like tobacco, which is very sensitive to an excess of water. This type of environmental stress might happen because of unpredictable season occurred in tropical region, including Indonesia. Global climate change as a consequence of rapid growing industries all over the world might responsible for this. This study aims to investigate the response of some Nicotiana tabacum varieties which are treated with periodic waterlogging stress. Some morphological aspects including plant height, stem diameter, leaves width and the emergence of adventitious roots were investigated. In addition, total chlorophyll content was also measured as physiological parameter. Three different varieties are used such as var. Jepon Pelakean, Jinten, Manilo and Morakot. Data were analyzed using One-Way Annova followed by Tukey post-hoc analysis. In all morphological aspects var. Jepon Pelakean demonstrated best responses compared to other varieties. Meanwhile, we observed a sharp decline of total chlorophyll content in var. Manilo under this periodic waterlogging stress. The overall results suggest that each variety of tobacco possess a specific mechanism against periodic waterlogging stress.

Data of root anatomical responses to periodic waterlogging stress of tobacco (Nicotiana tabacum) varieties

The data of root anatomical structure and the formation of aerenchyma tissues of five varieties of tobacco under waterlogging stress were obtained by modified paraffin method. Each tobacco varieties performed distinct anatomical adaptation response, including changes of cortical tissue, stele diameter, xylem diameter and the formation of aerenchyma under periodic waterlogging stress.

Response morphology and anatomy of tobacco (Nicotiana tabacum L.) plant on waterlogging

This study has conducted research on morphological and anatomical responses of some varieties of tobacco plants to waterlogging stress. Parameters measured were morphology, anatomy, and plants sensitivity index. Results were analyzed using two-way ANOVA followed by the Tukey test. The results show that waterlogging stress can reduce the growth of tobacco plants, including a decrease in plant height with the lowest value of 15.6 cm, root length reduction to the lowest value of 4.6 cm and plant dry weight reduction to the lowest value of 0.26 gr. But waterlogging stress can increase the number of adventitious roots with the highest value of 18.33. In addition, waterlogging stress can lead to the formation of aerenchyma tissue. The sensitivity index showed that plant varieties that are resistant to waterlogging stress are the varieties Kemloko 3 (index value of 0.03), varieties of Paiton 2 (index value of 0.18), and the varieties Kemloko 2 (index value of 0.42).

Traditional pattern of cashew cultivation : A lesson from Sumenep-Madura, Indonesia

Belonging to the Anacardiaceae family, the cashew tree (Anacardium Occidentale, Linn.) is one of the important tropical plants that possess high economic value. This plant is commonly grown in Indonesian regions including Sumenep, Madura, where the red sandy loam type of soil is commonly present. This study aims to obtain rough data on the pattern of cashew cultivation and identify the cashew cultivation knowledge of local communities. Data were taken in Bringin village, Sumenep-Madura. Our field survey showed that the cashew’s cultivation pattern in this village applies the so-called traditional organic farming. Cashew trees are planted along the boundaries of the owner’s farm field, functioning as a fence of their farm. Nevertheless, our survey also indicated that this pattern of cultivation is still below standard of cultivation. The planting distance between the cashew trees with one another is relatively close (< 5 meters), causing the cashew branches to overlap with each other. Moreover, we observed...