Udy Olshevsky
Hebrew University of Jerusalem
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Featured researches published by Udy Olshevsky.
Journal of General Virology | 1967
Yechiel Becker; Udy Olshevsky; Julia Levitt
Summary Arginine is essential for the replication of herpes simplex virus. Omission of arginine from the culture medium did not affect the synthesis of viral DNA but prevented the formation of virions. Addition of arginine immediately stimulated protein synthesis which was followed by virus formation.
Virology | 1970
Udy Olshevsky; Yechiel Becker
Abstract The protein composition of Herpes simplex viral capsids, unenveloped and enveloped virions was determined. Nine proteins (designated I to IX), with molecular weights ranging from 110,000 to 24,000 daltons, were identified in the enveloped virions. The analysis of empty viral capsids demonstrated the presence of two proteins (II and VIII). In addition to the capsid proteins, the naked virions contain two additional proteins (VI and VII). Protein VII was identified as the arginine-rich protein of the virus core. On maturation, lipid envelopes which contain three virus specific proteins (III, IV, and V) were attached to the naked virions. These three proteins were found to be labeled with radioactive glucosamine. The localization of protein III in the outer viral envelope was deduced from the complete removal of the protein from the virions after treatment with Nonidet P-40.
Virology | 1967
Udy Olshevsky; Julia Levitt; Yechiel Becker
Abstract The synthesis and assembly of DNA and coat proteins into herpes simplex virions were studied in infected BSC1 cells. Viral DNA synthesized in the nuclei of the infected cells is incorporated into mature virions within 2 hours. The viral proteins are synthesized in the cytoplasm of the infected cells and are transported into the nuclei, where they appear first in the soluble fraction, later in the viral capsids (subvirions), and within 2 hours in the mature herpes virions.
Virology | 1973
Menachem Gordin; Udy Olshevsky; Herbert S. Rosenkranz; Yechiel Becker
Denaturation of Herpes Simplex virus DNA, by heat and alkali treatment, results in the fragmentation of the viral DNA. Formamide denaturation of the viral DNA yields intact single-stranded DNA molecules.
Virology | 1975
Joseph Shlomai; Yael Asher; Yitzhak J. Gordon; Udy Olshevsky; Yechiel Becker
Abstract Treatment of herpes simplex virus-infected cells with ZnSO 4 (0.2 m M ) inhibited the synthesis of viral DNA. A similar concentration of ZnSO 4 had no effect on DNA synthesis in uninfected cells. In vitro analyses revealed a marked depression of the DNA-polymerase activity in nuclei obtained from zinc-treated infected cells.
Intervirology | 1974
Yechiel Becker; Joseph Shlomai; Yael Asher; Eynat Weinberg; Yafa Cohen; Udy Olshevsky; Moshe Kotler
Two cell lines [R(B77) and XC] of Rous sarcoma virus-transformed rat cells were infected with herpes simplex virus type 1 (HSV-1) grown in BSC-1 cells. Analysis of the DNA species synthesized in XC, R
Nature | 1973
Moshe Kotler; Eynat Weinberg; Osnat Haspel; Udy Olshevsky; Yechiel Becker
Nature | 1970
Udy Olshevsky; Yechiel Becker
Virology | 1972
Udy Olshevsky; Yechiel Becker
Intervirology | 1974
Yechiel Becker; Joseph Shlomai; Yael Asher; Eynat Weinberg; Yafa Cohen; Udy Olshevsky; Moshe Kotler; Joseph L. Melnick; Isamu Tagaya; Herdis von Magnus; Sally B. Padhi; Eric F. Eikenberry; Theodore Chase; Tam S. David-West; Christopher C. Payne; James Kalmakoff