Udy Olshevsky

The role of arginine in the replication of herpes simplex virus.

Summary Arginine is essential for the replication of herpes simplex virus. Omission of arginine from the culture medium did not affect the synthesis of viral DNA but prevented the formation of virions. Addition of arginine immediately stimulated protein synthesis which was followed by virus formation.

Herpes simplex virus structural proteins.

Abstract The protein composition of Herpes simplex viral capsids, unenveloped and enveloped virions was determined. Nine proteins (designated I to IX), with molecular weights ranging from 110,000 to 24,000 daltons, were identified in the enveloped virions. The analysis of empty viral capsids demonstrated the presence of two proteins (II and VIII). In addition to the capsid proteins, the naked virions contain two additional proteins (VI and VII). Protein VII was identified as the arginine-rich protein of the virus core. On maturation, lipid envelopes which contain three virus specific proteins (III, IV, and V) were attached to the naked virions. These three proteins were found to be labeled with radioactive glucosamine. The localization of protein III in the outer viral envelope was deduced from the complete removal of the protein from the virions after treatment with Nonidet P-40.

Studies on the synthesis of herpes simplex virions.

Abstract The synthesis and assembly of DNA and coat proteins into herpes simplex virions were studied in infected BSC1 cells. Viral DNA synthesized in the nuclei of the infected cells is incorporated into mature virions within 2 hours. The viral proteins are synthesized in the cytoplasm of the infected cells and are transported into the nuclei, where they appear first in the soluble fraction, later in the viral capsids (subvirions), and within 2 hours in the mature herpes virions.

Studies on Herpes Simplex virus DNA: Denaturation properties

Denaturation of Herpes Simplex virus DNA, by heat and alkali treatment, results in the fragmentation of the viral DNA. Formamide denaturation of the viral DNA yields intact single-stranded DNA molecules.

Effect of zinc ions on the synthesis of herpes simplex virus DNA in infected BSC-1 cells

Abstract Treatment of herpes simplex virus-infected cells with ZnSO 4 (0.2 m M ) inhibited the synthesis of viral DNA. A similar concentration of ZnSO 4 had no effect on DNA synthesis in uninfected cells. In vitro analyses revealed a marked depression of the DNA-polymerase activity in nuclei obtained from zinc-treated infected cells.

Interaction of herpes simplex virus type 1 with Rous sarcoma virus-transformed rat cells [XC and R(B77) cell lines].

Two cell lines [R(B77) and XC] of Rous sarcoma virus-transformed rat cells were infected with herpes simplex virus type 1 (HSV-1) grown in BSC-1 cells. Analysis of the DNA species synthesized in XC, R